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אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Production of diagnostic antibodies to plant viruses, utilizing denatured coat proteins and booster immunization with partially purified virus
Year:
1999
Source of publication :
Journal of Plant Pathology
Authors :
בקלמן, ילנה
;
.
בר-יוסף, משה
;
.
גרה, עבדאללה
;
.
כהן, יעקב
;
.
קריצמן, ענת
;
.
Volume :
81
Co-Authors:
Gera, A., Department of Virology, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Cohen, J., Department of Virology, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Kritzman, A., Department of Virology, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Beckelman, E., Department of Virology, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Bar-Joseph, M., Department of Virology, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Facilitators :
From page:
189
To page:
192
(
Total pages:
4
)
Abstract:
Groups of rabbits were immunized intramuscularly with either partially purified or denatured Iris Yellow Spot Virus (IYSV) nucleocapsid protein (NC) excised from SDS-polyacrylamide gels. Four injections were given at weekly intervals. Two weeks later, booster injections were given either with partially purified preparations of native virions or with denatured NC. Twelve days after the booster, rabbits were bled and the binding capacity of antisera was examined by decoration tests, Western blotting and ELISA. Diagnostic antibodies which could specifically bind to virus particles were obtained. Higher ELISA values were obtained when the booster injection contained partially purified IYSV, rather than denatured NC. Similar results were obtained with Potato Virus Y.
Note:
Related Files :
Coat protein
Decoration
Diagnostic antibodies
ELISA
Immunoblot
Iris Yellow Spot
Potato virus Y
עוד תגיות
תוכן קשור
More details
DOI :
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
24806
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:10
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Scientific Publication
Production of diagnostic antibodies to plant viruses, utilizing denatured coat proteins and booster immunization with partially purified virus
81
Gera, A., Department of Virology, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Cohen, J., Department of Virology, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Kritzman, A., Department of Virology, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Beckelman, E., Department of Virology, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Bar-Joseph, M., Department of Virology, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Production of diagnostic antibodies to plant viruses, utilizing denatured coat proteins and booster immunization with partially purified virus
Groups of rabbits were immunized intramuscularly with either partially purified or denatured Iris Yellow Spot Virus (IYSV) nucleocapsid protein (NC) excised from SDS-polyacrylamide gels. Four injections were given at weekly intervals. Two weeks later, booster injections were given either with partially purified preparations of native virions or with denatured NC. Twelve days after the booster, rabbits were bled and the binding capacity of antisera was examined by decoration tests, Western blotting and ELISA. Diagnostic antibodies which could specifically bind to virus particles were obtained. Higher ELISA values were obtained when the booster injection contained partially purified IYSV, rather than denatured NC. Similar results were obtained with Potato Virus Y.
Scientific Publication
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