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Post-mortem semen cryopreservation and characterization in two different endangered gazelle species (Gazella gazella and Gazella dorcas) and one subspecies (Gazella gazelle acaiae)
Year:
2006
Source of publication :
Theriogenology
Authors :
גסיטוע, חיים
;
.
Volume :
66
Co-Authors:
Saragusty, J., Department of Animal Sciences, Faculty of Agricultural, Food and Environmental Quality Sciences, P.O. Box 12, Rehovot, 76000, Israel, Institute of Animal Science, Agriculture Research Organization, The Volcani Center, P.O. Box 6, Bet-Dagan, 50250, Israel
Gacitua, H., Institute of Animal Science, Agriculture Research Organization, The Volcani Center, P.O. Box 6, Bet-Dagan, 50250, Israel
King, R., Israel Nature and Parks Authority, 3 Am Ve'Olamo Street, Jerusalem, 95463, Israel
Arav, A., Institute of Animal Science, Agriculture Research Organization, The Volcani Center, P.O. Box 6, Bet-Dagan, 50250, Israel
Facilitators :
From page:
775
To page:
784
(
Total pages:
10
)
Abstract:
Both Gazella gazella and Gazella dorcas are endangered species with continually dwindling population size, yet basic knowledge on their spermatozoa is missing. Semen collected post-mortem (PM) from the cauda epididymis of five adult gazelles (three Gazella gazella gazella, one Gazella gazella acaiae and one G. dorcas) was cryopreserved using directional freezing of large volumes (8 mL) with egg-yolk-free extender. Sperm size measurements and SYBR-14/propodium iodide (PI) viability stain validation for use in gazelles were conducted. Post-thaw characterization included motility, viability, acrosome damage evaluation, computerized motility characterization and morphology and sperm motility index (SMI) was calculated. Extracted sperm motility was 71.67 ± 11.67% (mean ± S.E.M.). Post-thaw motility ranged between 15% and 63%, viability was 57.49 ± 3.24%, intact acrosome was detected in 63.74 ± 2.6% (median 64.8%, upper/lower quartiles 71.79%, 61.82%), and normal morphology ranged between 41% and 63%. Motility characterization showed two sub-groups-highly active and progressively motile spermatozoa with SMI of 62.75 ± 0.38 and low activity and poorly progressive with SMI of 46.16 ± 1.53. Our results indicate that PM preservation of gazelle spermatozoa with satisfactory post-thaw viability is possible and cryobanking is achievable. © 2006 Elsevier Inc. All rights reserved.
Note:
Related Files :
cryopreservation
Epididymal sperm
Gazella
Gazella dorcas
Gazella gazella
Gazelle
Genome resource bank (GRB)
Spermatozoa
עוד תגיות
תוכן קשור
More details
DOI :
10.1016/j.theriogenology.2006.01.055
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
24822
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:10
You may also be interested in
Scientific Publication
Post-mortem semen cryopreservation and characterization in two different endangered gazelle species (Gazella gazella and Gazella dorcas) and one subspecies (Gazella gazelle acaiae)
66
Saragusty, J., Department of Animal Sciences, Faculty of Agricultural, Food and Environmental Quality Sciences, P.O. Box 12, Rehovot, 76000, Israel, Institute of Animal Science, Agriculture Research Organization, The Volcani Center, P.O. Box 6, Bet-Dagan, 50250, Israel
Gacitua, H., Institute of Animal Science, Agriculture Research Organization, The Volcani Center, P.O. Box 6, Bet-Dagan, 50250, Israel
King, R., Israel Nature and Parks Authority, 3 Am Ve'Olamo Street, Jerusalem, 95463, Israel
Arav, A., Institute of Animal Science, Agriculture Research Organization, The Volcani Center, P.O. Box 6, Bet-Dagan, 50250, Israel
Post-mortem semen cryopreservation and characterization in two different endangered gazelle species (Gazella gazella and Gazella dorcas) and one subspecies (Gazella gazelle acaiae)
Both Gazella gazella and Gazella dorcas are endangered species with continually dwindling population size, yet basic knowledge on their spermatozoa is missing. Semen collected post-mortem (PM) from the cauda epididymis of five adult gazelles (three Gazella gazella gazella, one Gazella gazella acaiae and one G. dorcas) was cryopreserved using directional freezing of large volumes (8 mL) with egg-yolk-free extender. Sperm size measurements and SYBR-14/propodium iodide (PI) viability stain validation for use in gazelles were conducted. Post-thaw characterization included motility, viability, acrosome damage evaluation, computerized motility characterization and morphology and sperm motility index (SMI) was calculated. Extracted sperm motility was 71.67 ± 11.67% (mean ± S.E.M.). Post-thaw motility ranged between 15% and 63%, viability was 57.49 ± 3.24%, intact acrosome was detected in 63.74 ± 2.6% (median 64.8%, upper/lower quartiles 71.79%, 61.82%), and normal morphology ranged between 41% and 63%. Motility characterization showed two sub-groups-highly active and progressively motile spermatozoa with SMI of 62.75 ± 0.38 and low activity and poorly progressive with SMI of 46.16 ± 1.53. Our results indicate that PM preservation of gazelle spermatozoa with satisfactory post-thaw viability is possible and cryobanking is achievable. © 2006 Elsevier Inc. All rights reserved.
Scientific Publication
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