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פותח על ידי קלירמאש פתרונות בע"מ -
Quantitative metabolic profiling by 1-dimensional 1H-NMR analyses: Application to plant genetics and functional genomics
Year:
2004
Source of publication :
Functional Plant Biology
Authors :
גרנות, דוד
;
.
Volume :
31
Co-Authors:
Moing, A., U. Rech. Sur Les Especes F., INRA, Université de Bordeaux 2, BP 81, F-33 883 Villenave d'Ornon, Cedex, France, UMR Physiol. et Biotech. Vég., Inst. Natl. de la Rech. Agronomique, Centre de Bordeaux, BP 81, F-33 883 Villenave d'Ornon, Cedex, France
Maucourt, M., UMR Physiol. et Biotech. Vég., INRA, Université de Bordeaux 2, BP 81, F-33 883 Villenave d'Ornon, Cedex, France
Renaud, C., U. Rech. Sur Les Especes F., INRA, Université de Bordeaux 2, BP 81, F-33 883 Villenave d'Ornon, Cedex, France
Gaudillère, M., UMR Physiol. et Biotech. Vég., INRA, Université de Bordeaux 2, BP 81, F-33 883 Villenave d'Ornon, Cedex, France
Brouquisse, R., UMR Physiol. et Biotech. Vég., INRA, Université de Bordeaux 2, BP 81, F-33 883 Villenave d'Ornon, Cedex, France
Lebouteiller, B., Inst. de Biotechnologie des Plantes, UMR CNRS 8618, Université Paris XI Sud, F-91 405 Orsay Cedex, France
Gousset-Dupont, A., Inst. de Biotechnologie des Plantes, UMR CNRS 8618, Université Paris XI Sud, F-91 405 Orsay Cedex, France
Vidal, J., Inst. de Biotechnologie des Plantes, UMR CNRS 8618, Université Paris XI Sud, F-91 405 Orsay Cedex, France
Granot, D., Institute of Field and Garden Crops, Agricultural Research Organisation, Volcani Center, Bet Dagan 50 250, Israel
Denoyes-Rothan, B., U. Rech. Sur Les Especes F., INRA, Université de Bordeaux 2, BP 81, F-33 883 Villenave d'Ornon, Cedex, France
Lerceteau-Köhler, E., CIREF, Lanxade, F-24 130 Prigonrieux, France
Rolin, D., UMR Physiol. et Biotech. Vég., INRA, Université de Bordeaux 2, BP 81, F-33 883 Villenave d'Ornon, Cedex, France
Facilitators :
From page:
889
To page:
902
(
Total pages:
14
)
Abstract:
Metabolic profiling by 1-dimensional (1-D) 1H-nuclear magnetic resonance (NMR) was tested for absolute quantification of soluble sugars, organic acids, amino acids and some secondary metabolites in fruit, roots and leaves. The metabolite responsible for each peak of the 1H-NMR spectra was identified from spectra of pure compounds. Peak identity was confirmed by the addition of a small amount of commercially-available pure substance. 1H-NMR spectra acquisition was automated. 1H-NMR absolute quantification was performed with a synthesised electronic reference signal and validated by comparison with enzymatic or HPLC analyses; the correlation coefficients between 1H-NMR data and enzymatic or HPLC data were highly significant. Depending on the species and tissues, 14-17 metabolites could be quantified with 15-25 min acquisition time. The detection limit was approximately 1-9 μg in the NMR tube, depending on the compound. Quantitative data were used for (1) a genetic study of strawberry fruit quality, (2) a functional study of tomato transformants overexpressing hexokinase and (3) a study of Arabidopsis phosphoenolpyruvate carboxylase transformants with several lines showing decreased activity of the enzyme. Biochemical phenotyping of the fruits of a strawberry offspring allowed the detection of quantitative trait loci (QTL) controlling fruit quality. Comparison of the roots of wild types and hexokinase tomato transformants using principal component analysis of metabolic profiles revealed that environmental factors, i.e. culture conditions, can significantly modify the metabolic status of plants and thus hide or emphasise the expression of a given genetic background. The decrease in phosphoenolpyruvate carboxylase activity (up to 75%) in Arabidopsis transformants impacted on the metabolic profiles without compromising plant growth, thus supporting the idea that the enzyme has a low influence on the carbon flux through the anaplerotic pathway.
Note:
Related Files :
Amino Acids
arabidopsis
enzymes
genetic engineering
Genetics
metabolism
metabolites
Metabolomics
Plants
עוד תגיות
תוכן קשור
More details
DOI :
10.1071/FP04066
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
24853
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:10
Scientific Publication
Quantitative metabolic profiling by 1-dimensional 1H-NMR analyses: Application to plant genetics and functional genomics
31
Moing, A., U. Rech. Sur Les Especes F., INRA, Université de Bordeaux 2, BP 81, F-33 883 Villenave d'Ornon, Cedex, France, UMR Physiol. et Biotech. Vég., Inst. Natl. de la Rech. Agronomique, Centre de Bordeaux, BP 81, F-33 883 Villenave d'Ornon, Cedex, France
Maucourt, M., UMR Physiol. et Biotech. Vég., INRA, Université de Bordeaux 2, BP 81, F-33 883 Villenave d'Ornon, Cedex, France
Renaud, C., U. Rech. Sur Les Especes F., INRA, Université de Bordeaux 2, BP 81, F-33 883 Villenave d'Ornon, Cedex, France
Gaudillère, M., UMR Physiol. et Biotech. Vég., INRA, Université de Bordeaux 2, BP 81, F-33 883 Villenave d'Ornon, Cedex, France
Brouquisse, R., UMR Physiol. et Biotech. Vég., INRA, Université de Bordeaux 2, BP 81, F-33 883 Villenave d'Ornon, Cedex, France
Lebouteiller, B., Inst. de Biotechnologie des Plantes, UMR CNRS 8618, Université Paris XI Sud, F-91 405 Orsay Cedex, France
Gousset-Dupont, A., Inst. de Biotechnologie des Plantes, UMR CNRS 8618, Université Paris XI Sud, F-91 405 Orsay Cedex, France
Vidal, J., Inst. de Biotechnologie des Plantes, UMR CNRS 8618, Université Paris XI Sud, F-91 405 Orsay Cedex, France
Granot, D., Institute of Field and Garden Crops, Agricultural Research Organisation, Volcani Center, Bet Dagan 50 250, Israel
Denoyes-Rothan, B., U. Rech. Sur Les Especes F., INRA, Université de Bordeaux 2, BP 81, F-33 883 Villenave d'Ornon, Cedex, France
Lerceteau-Köhler, E., CIREF, Lanxade, F-24 130 Prigonrieux, France
Rolin, D., UMR Physiol. et Biotech. Vég., INRA, Université de Bordeaux 2, BP 81, F-33 883 Villenave d'Ornon, Cedex, France
Quantitative metabolic profiling by 1-dimensional 1H-NMR analyses: Application to plant genetics and functional genomics
Metabolic profiling by 1-dimensional (1-D) 1H-nuclear magnetic resonance (NMR) was tested for absolute quantification of soluble sugars, organic acids, amino acids and some secondary metabolites in fruit, roots and leaves. The metabolite responsible for each peak of the 1H-NMR spectra was identified from spectra of pure compounds. Peak identity was confirmed by the addition of a small amount of commercially-available pure substance. 1H-NMR spectra acquisition was automated. 1H-NMR absolute quantification was performed with a synthesised electronic reference signal and validated by comparison with enzymatic or HPLC analyses; the correlation coefficients between 1H-NMR data and enzymatic or HPLC data were highly significant. Depending on the species and tissues, 14-17 metabolites could be quantified with 15-25 min acquisition time. The detection limit was approximately 1-9 μg in the NMR tube, depending on the compound. Quantitative data were used for (1) a genetic study of strawberry fruit quality, (2) a functional study of tomato transformants overexpressing hexokinase and (3) a study of Arabidopsis phosphoenolpyruvate carboxylase transformants with several lines showing decreased activity of the enzyme. Biochemical phenotyping of the fruits of a strawberry offspring allowed the detection of quantitative trait loci (QTL) controlling fruit quality. Comparison of the roots of wild types and hexokinase tomato transformants using principal component analysis of metabolic profiles revealed that environmental factors, i.e. culture conditions, can significantly modify the metabolic status of plants and thus hide or emphasise the expression of a given genetic background. The decrease in phosphoenolpyruvate carboxylase activity (up to 75%) in Arabidopsis transformants impacted on the metabolic profiles without compromising plant growth, thus supporting the idea that the enzyme has a low influence on the carbon flux through the anaplerotic pathway.
Scientific Publication
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