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פותח על ידי קלירמאש פתרונות בע"מ -
Cadherin-mediated transmembrane interactions
Year:
1998
Source of publication :
Cell Adhesion and Communication
Authors :
שדות, עינת
;
.
Volume :
6
Co-Authors:
Levenberg, S., Department of Molecular Cell Biology, The Weizmann Institute of Science, Rehovot, Israel
Sadot, E., Department of Molecular Cell Biology, The Weizmann Institute of Science, Rehovot, Israel
Goichberg, P., Department of Molecular Cell Biology, The Weizmann Institute of Science, Rehovot, Israel
Geiger, B., Department of Molecular Cell Biology, The Weizmann Institute of Science, Rehovot, Israel
Facilitators :
From page:
161
To page:
170
(
Total pages:
10
)
Abstract:
We show in this study that cadherin ligands, either soluble or immobilized on different surfaces, can bind to cells carrying a compatible cadherin and induce long-range signals which affect cell adhesion and dynamics. Addition of recombinant N-cadherin extracellular domain (NEC) to CHO cells expressing N-cadherin (FL4) greatly enhanced the calcium-dependent aggregation of the cells and blocked their migration into an 'in vitro wound'. Monoclonal antibody which blocks cadherin interactions inhibited the aggregation of suspended FL4 cells and facilitated the 'wound closure'. As previously shown synthetic beads coupled to NEC interacted specifically with the surface of FL4 cells and significantly enhanced the formation of adherens junctions. This effect was obtained also with the parental CHO cells, which contain low levels of N-cadherin, and in additional N-cadherin expressing cells such as cultured myoblasts. We further show here that stimulation of adhesion is not affected by the geometry of the NEC-bound surface and that cells plated on flat NEC-coated substratum also develop enhanced adherens junctions. Interaction of cells expressing low levels of endogenous N-cadherin, such as CHO cells with surface-immobilized N-cadherin ligands had a prominent effect also on the total level of N-cadherin and β-catenin in the cells, probably due to stabilization of the cadherin-catenin complex by the interaction with the external surface.
Note:
Related Files :
alpha Catenin
animal cell
Animals
Cadherins
Cell Adhesion
cell membrane
cho cell
Conference paper
myoblast
עוד תגיות
תוכן קשור
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DOI :
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר מתוך כינוס
;
.
Language:
אנגלית
Editors' remarks:
ID:
24901
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:11
Scientific Publication
Cadherin-mediated transmembrane interactions
6
Levenberg, S., Department of Molecular Cell Biology, The Weizmann Institute of Science, Rehovot, Israel
Sadot, E., Department of Molecular Cell Biology, The Weizmann Institute of Science, Rehovot, Israel
Goichberg, P., Department of Molecular Cell Biology, The Weizmann Institute of Science, Rehovot, Israel
Geiger, B., Department of Molecular Cell Biology, The Weizmann Institute of Science, Rehovot, Israel
Cadherin-mediated transmembrane interactions
We show in this study that cadherin ligands, either soluble or immobilized on different surfaces, can bind to cells carrying a compatible cadherin and induce long-range signals which affect cell adhesion and dynamics. Addition of recombinant N-cadherin extracellular domain (NEC) to CHO cells expressing N-cadherin (FL4) greatly enhanced the calcium-dependent aggregation of the cells and blocked their migration into an 'in vitro wound'. Monoclonal antibody which blocks cadherin interactions inhibited the aggregation of suspended FL4 cells and facilitated the 'wound closure'. As previously shown synthetic beads coupled to NEC interacted specifically with the surface of FL4 cells and significantly enhanced the formation of adherens junctions. This effect was obtained also with the parental CHO cells, which contain low levels of N-cadherin, and in additional N-cadherin expressing cells such as cultured myoblasts. We further show here that stimulation of adhesion is not affected by the geometry of the NEC-bound surface and that cells plated on flat NEC-coated substratum also develop enhanced adherens junctions. Interaction of cells expressing low levels of endogenous N-cadherin, such as CHO cells with surface-immobilized N-cadherin ligands had a prominent effect also on the total level of N-cadherin and β-catenin in the cells, probably due to stabilization of the cadherin-catenin complex by the interaction with the external surface.
Scientific Publication
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