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פותח על ידי קלירמאש פתרונות בע"מ -
Profiles of free and conjugated ecdysteroids and ecdysteroid acids during pupal‐adult development of Manduca sexta
Year:
1989
Authors :
סבובודה, ג'יימס
;
.
Volume :
12
Co-Authors:
Lozano, R., Insect and Nematode Hormone Laboratory, Agricultural Research Service, Usda, Beltsville, Maryland, United States
Thompson, M.J., Insect and Nematode Hormone Laboratory, Agricultural Research Service, Usda, Beltsville, Maryland, United States
Svoboda, J.A., Insect and Nematode Hormone Laboratory, Agricultural Research Service, Usda, Beltsville, Maryland, United States
Lusby, W.R., Insect and Nematode Hormone Laboratory, Agricultural Research Service, Usda, Beltsville, Maryland, United States
Facilitators :
From page:
63
To page:
74
(
Total pages:
12
)
Abstract:
The levels of individual free and conjugated ecdysteroids and ecdysteroid acids, labeled from [14C]cholesterol, in five different age groups of male Manduca sexta during pupal‐adult development were determined by HPLC. Eight free ecdysteroids, eight ecdysteroid phosphates, and two ecdysteroid acids were identified. Newly ecdysed pupae contained predominantly 3‐epiecdysteroids in each of the free, conjugated, and acidic ecdysteroid fractions. The titer of each ecdysteroid fraction rose sharply by day 4, and this was particularly noteworthy with respect to free ecdysone and 3‐epi‐20‐hydroxyecdysonoic acid. This stage demonstrated high degrees of ecdysone biosynthesis, oxidative catabolism, and phosphorylation. As development proceeded to day 16, total ecdysteroid titer remained constant; a decreasing free ecdysteroid titer was accompanieid by increasing titers of both conjugates and acids resulting from the metabolic processes of hydroxylation, oxidation, epimerization, and phosphorylation. The predominant metabolites throughout development were 3‐epi‐20‐hydroxyecdysonoic acid and the phosphate conjugates of 3‐epi‐20‐hydroxyecdysone and 3‐epi‐20,26‐dihydroxyecdysone. The ultimate inactivation of the ecdysteroids of M. sexta during pupal‐adult development is possibly mediated by two pairs of metabolically‐linked processes, one leading to a 3‐epiecdysteroid acid, and the other to 3‐epiecdysteroid phosphates. Copyright © 1989 Wiley‐Liss, Inc.
Note:
Related Files :
epiecdysteroid acids
epiecdysteroid phosphates
epimerization
HPLC
Hydroxylation
oxidation
עוד תגיות
תוכן קשור
More details
DOI :
10.1002/arch.940120106
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
24906
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:11
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Scientific Publication
Profiles of free and conjugated ecdysteroids and ecdysteroid acids during pupal‐adult development of Manduca sexta
12
Lozano, R., Insect and Nematode Hormone Laboratory, Agricultural Research Service, Usda, Beltsville, Maryland, United States
Thompson, M.J., Insect and Nematode Hormone Laboratory, Agricultural Research Service, Usda, Beltsville, Maryland, United States
Svoboda, J.A., Insect and Nematode Hormone Laboratory, Agricultural Research Service, Usda, Beltsville, Maryland, United States
Lusby, W.R., Insect and Nematode Hormone Laboratory, Agricultural Research Service, Usda, Beltsville, Maryland, United States
Profiles of free and conjugated ecdysteroids and ecdysteroid acids during pupal‐adult development of Manduca sexta
The levels of individual free and conjugated ecdysteroids and ecdysteroid acids, labeled from [14C]cholesterol, in five different age groups of male Manduca sexta during pupal‐adult development were determined by HPLC. Eight free ecdysteroids, eight ecdysteroid phosphates, and two ecdysteroid acids were identified. Newly ecdysed pupae contained predominantly 3‐epiecdysteroids in each of the free, conjugated, and acidic ecdysteroid fractions. The titer of each ecdysteroid fraction rose sharply by day 4, and this was particularly noteworthy with respect to free ecdysone and 3‐epi‐20‐hydroxyecdysonoic acid. This stage demonstrated high degrees of ecdysone biosynthesis, oxidative catabolism, and phosphorylation. As development proceeded to day 16, total ecdysteroid titer remained constant; a decreasing free ecdysteroid titer was accompanieid by increasing titers of both conjugates and acids resulting from the metabolic processes of hydroxylation, oxidation, epimerization, and phosphorylation. The predominant metabolites throughout development were 3‐epi‐20‐hydroxyecdysonoic acid and the phosphate conjugates of 3‐epi‐20‐hydroxyecdysone and 3‐epi‐20,26‐dihydroxyecdysone. The ultimate inactivation of the ecdysteroids of M. sexta during pupal‐adult development is possibly mediated by two pairs of metabolically‐linked processes, one leading to a 3‐epiecdysteroid acid, and the other to 3‐epiecdysteroid phosphates. Copyright © 1989 Wiley‐Liss, Inc.
Scientific Publication
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