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Genetic transformation with a derivative of rolC from Agrobacterium rhizogenes and treatment with α-aminoisobutyric acid produce similar phenotypes and reduce ethylene production and the accumulation of water-insoluble polyamine-hydroxycinnamic acid conjugates in tobacco flowers
Year:
1993
Source of publication :
Plant Science
Authors :
בן חיים, גוזל
;
.
Volume :
93
Co-Authors:
Martin-Tanguy, J., Laboratoire de Physioparasitologie, Station Amélioration des Plantes, INRA, 21034 Dijon Cedex, France
Corbineau, F., Physiologie Végétale Appliquée, Université Pierre et Marie Curie, Tour 53, 4 Place Jussieu, 75252 Cedex 05, France
Burtin, D., Laboratoire de Physioparasitologie, Station Amélioration des Plantes, INRA, 21034 Dijon Cedex, France
Ben-Hayyim, G., Laboratoire de Biologie de la Rhizosphère, Institut National de la Recherche Agronomique, 78026 Versailles Cedex, France
Tepfer, D., Laboratoire de Biologie de la Rhizosphère, Institut National de la Recherche Agronomique, 78026 Versailles Cedex, France
Facilitators :
From page:
63
To page:
76
(
Total pages:
14
)
Abstract:
Tobacco plants were either transformed by rolA (ORF 10) or rolC (ORF 12) from the Ri TL-DNA of Agrobacterium rhizogenes, or they were treated with an inhibitor of ethylene production, α-AIB (α-aminoisobutyric acid). We recorded changes in phenotype, ethylene production and the accumulation of di- and polyamines, tyramine and their derivatives in excised flowers, as a function of floral development. Both α-AIB and the rolC gene (under the control of the 35S promoter from the CaMV virus) caused male sterility and a reduction in flower size, accompanied by a depression in the accumulation of water-insoluble polyamine and tyramine conjugates and a reduction in ethylene production. In plants transformed by 35S-rolC, we also observed narrowed leaves, reduced internode distance and changes in root system architecture. Transformation by 35S-rolA caused leaf wrinkling, internode shortening and downward bending of stamen filaments. We discuss these observations in the light of proposed functions for rolA and rolC and known relationships between polyamine and ethylene metabolism. © 1993.
Note:
Related Files :
Agrobacterium rhizogenes
ethylene
flowering
genetic transformation
Polyamines
Ri TL-DNA
עוד תגיות
תוכן קשור
More details
DOI :
10.1016/0168-9452(93)90035-X
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
24908
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:11
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Scientific Publication
Genetic transformation with a derivative of rolC from Agrobacterium rhizogenes and treatment with α-aminoisobutyric acid produce similar phenotypes and reduce ethylene production and the accumulation of water-insoluble polyamine-hydroxycinnamic acid conjugates in tobacco flowers
93
Martin-Tanguy, J., Laboratoire de Physioparasitologie, Station Amélioration des Plantes, INRA, 21034 Dijon Cedex, France
Corbineau, F., Physiologie Végétale Appliquée, Université Pierre et Marie Curie, Tour 53, 4 Place Jussieu, 75252 Cedex 05, France
Burtin, D., Laboratoire de Physioparasitologie, Station Amélioration des Plantes, INRA, 21034 Dijon Cedex, France
Ben-Hayyim, G., Laboratoire de Biologie de la Rhizosphère, Institut National de la Recherche Agronomique, 78026 Versailles Cedex, France
Tepfer, D., Laboratoire de Biologie de la Rhizosphère, Institut National de la Recherche Agronomique, 78026 Versailles Cedex, France
Genetic transformation with a derivative of rolC from Agrobacterium rhizogenes and treatment with α-aminoisobutyric acid produce similar phenotypes and reduce ethylene production and the accumulation of water-insoluble polyamine-hydroxycinnamic acid conjugates in tobacco flowers
Tobacco plants were either transformed by rolA (ORF 10) or rolC (ORF 12) from the Ri TL-DNA of Agrobacterium rhizogenes, or they were treated with an inhibitor of ethylene production, α-AIB (α-aminoisobutyric acid). We recorded changes in phenotype, ethylene production and the accumulation of di- and polyamines, tyramine and their derivatives in excised flowers, as a function of floral development. Both α-AIB and the rolC gene (under the control of the 35S promoter from the CaMV virus) caused male sterility and a reduction in flower size, accompanied by a depression in the accumulation of water-insoluble polyamine and tyramine conjugates and a reduction in ethylene production. In plants transformed by 35S-rolC, we also observed narrowed leaves, reduced internode distance and changes in root system architecture. Transformation by 35S-rolA caused leaf wrinkling, internode shortening and downward bending of stamen filaments. We discuss these observations in the light of proposed functions for rolA and rolC and known relationships between polyamine and ethylene metabolism. © 1993.
Scientific Publication
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