Transgenic Research
Zrachya, A., Department of Genetics, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel, Department of Biological Chemistry, Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, Jerusalem 91904, Israel
Kumar, P.P., Department of Plant Biotechnology, School of Biotechnology, Madurai Kamaraj University, Madurai 625021, India
Ramakrishnan, U., Department of Plant Biotechnology, School of Biotechnology, Madurai Kamaraj University, Madurai 625021, India
Levy, Y., Department of Genetics, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Loyter, A., Department of Biological Chemistry, Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, Jerusalem 91904, Israel
Arazi, T., Department of Ornamental Horticulture, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Lapidot, M., Department of Genetics, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Gafni, Y., Department of Genetics, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
The coat protein (CP) of Tomato yellow leaf curl virus (TYLCV), encoded by the v1 gene, is the only known component of the viral capsid. In addition, the CP plays a role in the virus transport into the host cell nucleus where viral genes are replicated and transcribed. In this study, we analyzed the effect of small interfering double-stranded RNAs (siRNAs), derived from an intron-hairpin RNA (ihpRNA) construct and targeting the v1 gene product, on CP accumulation. Transient assays involving agroinfiltration of the CP-silencing construct followed by infiltration of a fused GFP-CP (green fluorescent protein-coat protein) gene showed down-regulation of GFP expression in Nicotiana benthamiana. Some of the transgenic tomato plants (cv. Micro-Tom), expressing the siRNA targeted against the TYLCV CP gene, did not show disease symptoms 7 weeks post-inoculation with the virus, while non-transgenic control plants were infected within 2 weeks post inoculation. The present study demonstrates, for the first time, that siRNA targeted against the CP of TYLCV can confer resistance to the virus in transgenic tomato plants, thereby enabling flowering and fruit production. © 2006 Springer Science+Business Media B.V.
פותח על ידי קלירמאש פתרונות בע"מ -
הספר "אוצר וולקני"
אודות
תנאי שימוש
Production of siRNA targeted against TYLCV coat protein transcripts leads to silencing of its expression and resistance to the virus
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Zrachya, A., Department of Genetics, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel, Department of Biological Chemistry, Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, Jerusalem 91904, Israel
Kumar, P.P., Department of Plant Biotechnology, School of Biotechnology, Madurai Kamaraj University, Madurai 625021, India
Ramakrishnan, U., Department of Plant Biotechnology, School of Biotechnology, Madurai Kamaraj University, Madurai 625021, India
Levy, Y., Department of Genetics, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Loyter, A., Department of Biological Chemistry, Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, Jerusalem 91904, Israel
Arazi, T., Department of Ornamental Horticulture, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Lapidot, M., Department of Genetics, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Gafni, Y., Department of Genetics, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Production of siRNA targeted against TYLCV coat protein transcripts leads to silencing of its expression and resistance to the virus
The coat protein (CP) of Tomato yellow leaf curl virus (TYLCV), encoded by the v1 gene, is the only known component of the viral capsid. In addition, the CP plays a role in the virus transport into the host cell nucleus where viral genes are replicated and transcribed. In this study, we analyzed the effect of small interfering double-stranded RNAs (siRNAs), derived from an intron-hairpin RNA (ihpRNA) construct and targeting the v1 gene product, on CP accumulation. Transient assays involving agroinfiltration of the CP-silencing construct followed by infiltration of a fused GFP-CP (green fluorescent protein-coat protein) gene showed down-regulation of GFP expression in Nicotiana benthamiana. Some of the transgenic tomato plants (cv. Micro-Tom), expressing the siRNA targeted against the TYLCV CP gene, did not show disease symptoms 7 weeks post-inoculation with the virus, while non-transgenic control plants were infected within 2 weeks post inoculation. The present study demonstrates, for the first time, that siRNA targeted against the CP of TYLCV can confer resistance to the virus in transgenic tomato plants, thereby enabling flowering and fruit production. © 2006 Springer Science+Business Media B.V.
Scientific Publication