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פותח על ידי קלירמאש פתרונות בע"מ -
A comparative analysis of genetic variation in rootstocks and scions of old olive trees - a window into the history of olive cultivation practices and past genetic variation
Year:
2014
Source of publication :
BMC Plant Biology
Authors :
ברזני, עוז
;
.
דג, ארנון
;
.
חנין, ניר
;
.
טוגנדהפט, יזהר
;
.
Volume :
14
Co-Authors:
Barazani, O., Institute of Plant Sciences, Israel Plant Gene Bank, Agricultural Research Organization, Bet Dagan 50250, Israel
Westberg, E., Institut für Spezielle Botanik und Botanischer Garten, Johannes Gutenberg-Universität Mainz, D-55099 Mainz, Germany
Hanin, N., Institute of Plant Sciences, Israel Plant Gene Bank, Agricultural Research Organization, Bet Dagan 50250, Israel
Dag, A., Institute of Plant Sciences, Department of Fruit Tree Sciences, Agricultural Research Organization, Gilat Research Center, Gilat, Israel
Kerem, Z., Institute of Biochemistry, Food Science and Nutrition, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot 76100, Israel
Tugendhaft, Y., Institute of Plant Sciences, Department of Fruit Tree Sciences, Agricultural Research Organization, Gilat Research Center, Gilat, Israel, Institute of Biochemistry, Food Science and Nutrition, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot 76100, Israel
Hmidat, M., Integrated Rural Development (AIRD), Ramallah, Jerusalem Street, Al Nabali Building, P.O.Box 6, Ramallah, Israel
Hijawi, T., Integrated Rural Development (AIRD), Ramallah, Jerusalem Street, Al Nabali Building, P.O.Box 6, Ramallah, Israel
Kadereit, J.W., Institut für Spezielle Botanik und Botanischer Garten, Johannes Gutenberg-Universität Mainz, D-55099 Mainz, Germany
Facilitators :
From page:
To page:
(
Total pages:
1
)
Abstract:
Background: Past clonal propagation of olive trees is intimately linked to grafting. However, evidence on grafting in ancient trees is scarce, and not much is known about the source of plant material used for rootstocks. Here, the Simple Sequence Repeat (SSR) marker technique was used to study genetic diversity of rootstocks and scions in ancient olive trees from the Levant and its implications for past cultivation of olives. Leaf samples were collected from tree canopies (scions) and shoots growing from the trunk base (suckers). A total of 310 trees were sampled in 32 groves and analyzed with 14 SSR markers.Results: In 82.7% of the trees in which both scion and suckers could be genotyped, these were genetically different, and thus suckers were interpreted to represent the rootstock of grafted trees. Genetic diversity values were much higher among suckers than among scions, and 194 and 87 multi-locus genotypes (MLGs) were found in the two sample groups, respectively. Only five private alleles were found among scions, but 125 among suckers. A frequency analysis revealed a bimodal distribution of genetic distance among MLGs, indicating the presence of somatic mutations within clones. When assuming that MLGs differing by one mutation are identical, scion and sucker MLGs were grouped in 20 and 147 multi-locus lineages (MLLs). The majority of scions (90.0%) belonged to a single common MLL, whereas 50.5% of the suckers were single-sample MLLs. However, one MLL was specific to suckers and found in 63 (22.6%) of the samples.Conclusions: Our results provide strong evidence that the majority of olive trees in the study are grafted, that the large majority of scions belong to a single ancient cultivar containing somatic mutations, and that the widespread occurrence of one sucker genotype may imply rootstock selection. For the majority of grafted trees it seems likely that saplings were used as rootstocks; their genetic diversity probably is best explained as the result of a long history of sexual reproduction involving cultivated, feral and wild genotypes. © 2014 Barazani et al.
Note:
Related Files :
Genetic Loci
Genetics
Growth, Development and Aging
Israel
Olea
Olive cultivars
plant
tree
trees
עוד תגיות
תוכן קשור
More details
DOI :
10.1186/1471-2229-14-146
Article number:
146
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
24941
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:11
You may also be interested in
Scientific Publication
A comparative analysis of genetic variation in rootstocks and scions of old olive trees - a window into the history of olive cultivation practices and past genetic variation
14
Barazani, O., Institute of Plant Sciences, Israel Plant Gene Bank, Agricultural Research Organization, Bet Dagan 50250, Israel
Westberg, E., Institut für Spezielle Botanik und Botanischer Garten, Johannes Gutenberg-Universität Mainz, D-55099 Mainz, Germany
Hanin, N., Institute of Plant Sciences, Israel Plant Gene Bank, Agricultural Research Organization, Bet Dagan 50250, Israel
Dag, A., Institute of Plant Sciences, Department of Fruit Tree Sciences, Agricultural Research Organization, Gilat Research Center, Gilat, Israel
Kerem, Z., Institute of Biochemistry, Food Science and Nutrition, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot 76100, Israel
Tugendhaft, Y., Institute of Plant Sciences, Department of Fruit Tree Sciences, Agricultural Research Organization, Gilat Research Center, Gilat, Israel, Institute of Biochemistry, Food Science and Nutrition, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot 76100, Israel
Hmidat, M., Integrated Rural Development (AIRD), Ramallah, Jerusalem Street, Al Nabali Building, P.O.Box 6, Ramallah, Israel
Hijawi, T., Integrated Rural Development (AIRD), Ramallah, Jerusalem Street, Al Nabali Building, P.O.Box 6, Ramallah, Israel
Kadereit, J.W., Institut für Spezielle Botanik und Botanischer Garten, Johannes Gutenberg-Universität Mainz, D-55099 Mainz, Germany
A comparative analysis of genetic variation in rootstocks and scions of old olive trees - a window into the history of olive cultivation practices and past genetic variation
Background: Past clonal propagation of olive trees is intimately linked to grafting. However, evidence on grafting in ancient trees is scarce, and not much is known about the source of plant material used for rootstocks. Here, the Simple Sequence Repeat (SSR) marker technique was used to study genetic diversity of rootstocks and scions in ancient olive trees from the Levant and its implications for past cultivation of olives. Leaf samples were collected from tree canopies (scions) and shoots growing from the trunk base (suckers). A total of 310 trees were sampled in 32 groves and analyzed with 14 SSR markers.Results: In 82.7% of the trees in which both scion and suckers could be genotyped, these were genetically different, and thus suckers were interpreted to represent the rootstock of grafted trees. Genetic diversity values were much higher among suckers than among scions, and 194 and 87 multi-locus genotypes (MLGs) were found in the two sample groups, respectively. Only five private alleles were found among scions, but 125 among suckers. A frequency analysis revealed a bimodal distribution of genetic distance among MLGs, indicating the presence of somatic mutations within clones. When assuming that MLGs differing by one mutation are identical, scion and sucker MLGs were grouped in 20 and 147 multi-locus lineages (MLLs). The majority of scions (90.0%) belonged to a single common MLL, whereas 50.5% of the suckers were single-sample MLLs. However, one MLL was specific to suckers and found in 63 (22.6%) of the samples.Conclusions: Our results provide strong evidence that the majority of olive trees in the study are grafted, that the large majority of scions belong to a single ancient cultivar containing somatic mutations, and that the widespread occurrence of one sucker genotype may imply rootstock selection. For the majority of grafted trees it seems likely that saplings were used as rootstocks; their genetic diversity probably is best explained as the result of a long history of sexual reproduction involving cultivated, feral and wild genotypes. © 2014 Barazani et al.
Scientific Publication
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