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פותח על ידי קלירמאש פתרונות בע"מ -
Changes in the activity of the alternative oxidase in Orobanche seeds during conditioning and their possible physiological function
Year:
2003
Source of publication :
Phytochemistry
Authors :
יואל, דניאל
;
.
Volume :
64
Co-Authors:
Bar Nun, N., Department of Botany, Hebrew University of Jerusalem, Jerusalem 91904, Israel
Plakhine, D., Department of Weed Research, Agricultural Organization, Newe-Ya'ar Research Center, Ramat Yishai 30095, Israel
Joel, D.M., Department of Weed Research, Agricultural Organization, Newe-Ya'ar Research Center, Ramat Yishai 30095, Israel
Mayer, A.M., Department of Botany, Hebrew University of Jerusalem, Jerusalem 91904, Israel
Facilitators :
From page:
235
To page:
241
(
Total pages:
7
)
Abstract:
The appearance of the activity of the cyanide insensitive, alternative oxidase (AOX), pathway of oxygen uptake was followed in seeds of Orobanche aegyptiaca during conditioning. The pathway becomes operative during conditioning, up to day three as determined by inhibition of oxygen uptake of the seeds by propyl gallate. At the same time an increasing percentage of oxygen uptake is insensitive to cyanide and an increased oxygen uptake, responsive to propyl gallate, is induced by brief salicylic acid treatment of seeds. By day six of conditioning, these responses decrease and the AOX pathway could not be detected in germinating seeds, after treatment with a germination stimulant. These results were confirmed by following the reaction of extracts of fractions enriched with mitochondria from the conditioned seeds, using a specific antibody against AOX. Treatment of the seeds with inhibitors of AOX during conditioning significantly inhibited their subsequent germination. Addition of hydrogen peroxide after 4 and 7 days of conditioning resulted in reduced germination. In addition treatment of seed with propyl or octyl gallate during conditioning reduced the infection of tomato plants by Orobanche seeds and the development of tubercles of the parasite on the host roots. These results together indicate that the operation of AOX during conditioning has a significant function on the subsequent germination behaviour and pathogenicity of the root parasite. Some potential practical applications of these findings are discussed. © 2003 Elsevier Ltd. All rights reserved.
Note:
Related Files :
AOX protein
germination
Inhibition of AOX
Orobanche
plant
Plant Diseases
reactive oxygen species
seeds
עוד תגיות
תוכן קשור
More details
DOI :
10.1016/S0031-9422(03)00165-1
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
24971
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:11
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Scientific Publication
Changes in the activity of the alternative oxidase in Orobanche seeds during conditioning and their possible physiological function
64
Bar Nun, N., Department of Botany, Hebrew University of Jerusalem, Jerusalem 91904, Israel
Plakhine, D., Department of Weed Research, Agricultural Organization, Newe-Ya'ar Research Center, Ramat Yishai 30095, Israel
Joel, D.M., Department of Weed Research, Agricultural Organization, Newe-Ya'ar Research Center, Ramat Yishai 30095, Israel
Mayer, A.M., Department of Botany, Hebrew University of Jerusalem, Jerusalem 91904, Israel
Changes in the activity of the alternative oxidase in Orobanche seeds during conditioning and their possible physiological function
The appearance of the activity of the cyanide insensitive, alternative oxidase (AOX), pathway of oxygen uptake was followed in seeds of Orobanche aegyptiaca during conditioning. The pathway becomes operative during conditioning, up to day three as determined by inhibition of oxygen uptake of the seeds by propyl gallate. At the same time an increasing percentage of oxygen uptake is insensitive to cyanide and an increased oxygen uptake, responsive to propyl gallate, is induced by brief salicylic acid treatment of seeds. By day six of conditioning, these responses decrease and the AOX pathway could not be detected in germinating seeds, after treatment with a germination stimulant. These results were confirmed by following the reaction of extracts of fractions enriched with mitochondria from the conditioned seeds, using a specific antibody against AOX. Treatment of the seeds with inhibitors of AOX during conditioning significantly inhibited their subsequent germination. Addition of hydrogen peroxide after 4 and 7 days of conditioning resulted in reduced germination. In addition treatment of seed with propyl or octyl gallate during conditioning reduced the infection of tomato plants by Orobanche seeds and the development of tubercles of the parasite on the host roots. These results together indicate that the operation of AOX during conditioning has a significant function on the subsequent germination behaviour and pathogenicity of the root parasite. Some potential practical applications of these findings are discussed. © 2003 Elsevier Ltd. All rights reserved.
Scientific Publication
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