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פותח על ידי קלירמאש פתרונות בע"מ -
Identification of a novel plant virus promoter using a potyvirus infectious clone
Year:
2000
Source of publication :
Virus Genes
Authors :
גאבה, ויקטור
;
.
גל-און, עמית
;
.
וולף, דליה
;
.
זלצר, אהרון
;
.
יונגזאנג, ואנג
;
.
שיה, שאודי
;
.
Volume :
20
Co-Authors:
Facilitators :
From page:
11
To page:
17
(
Total pages:
7
)
Abstract:
A putative promoter from the strawberry vein banding caulimovirus (SVBV) genome was identified by its ability to drive infection with full-length cDNA of the zucchini yellow mosaic RNA potyvirus (ZYMV). A high rate of infection was obtained with the cDNA under control of the SVBV promoter using particle bombardment technology. The SVBV promoter shows 60% homology to the cauliflower mosaic virus 35S promoter in the domain spanning the conserved motifs of CCACT (at -83) and the TATA box (at -31), to the transcription start. The 3'-end one-third of the putative promoter (328 bp) was sufficient to invoke full infectivity with the ZYMV clone, and drove transient reporter gene expression in Solanaceae and Cucurbitaceae transformed with a binary plant transformation vector. Stable expression of a reporter gene (GUS) under control of the truncated SVBV promoter was shown in transformed tobacco shoots in roots, leaves and stems.
Note:
Related Files :
Base Sequence
biolistic transformation
biotechnology
Cucurbitaceae
Solanaceae
virus infectivity
Zucchini yellow mosaic virus
עוד תגיות
תוכן קשור
More details
DOI :
10.1023/A:1008199805099
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
25010
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:11
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Identification of a novel plant virus promoter using a potyvirus infectious clone
20
Identification of a novel plant virus promoter using a potyvirus infectious clone
A putative promoter from the strawberry vein banding caulimovirus (SVBV) genome was identified by its ability to drive infection with full-length cDNA of the zucchini yellow mosaic RNA potyvirus (ZYMV). A high rate of infection was obtained with the cDNA under control of the SVBV promoter using particle bombardment technology. The SVBV promoter shows 60% homology to the cauliflower mosaic virus 35S promoter in the domain spanning the conserved motifs of CCACT (at -83) and the TATA box (at -31), to the transcription start. The 3'-end one-third of the putative promoter (328 bp) was sufficient to invoke full infectivity with the ZYMV clone, and drove transient reporter gene expression in Solanaceae and Cucurbitaceae transformed with a binary plant transformation vector. Stable expression of a reporter gene (GUS) under control of the truncated SVBV promoter was shown in transformed tobacco shoots in roots, leaves and stems.
Scientific Publication
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