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קהילה:
אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Molecular interactions between the biocontrol agent Metschnikowia fructicola and citrus fruit tissue and Penicillium digitatum
Year:
2014
Source of publication :
Acta Horticulturae
Authors :
דרובי, סמיר
;
.
לוין, ילנה
;
.
סלע, נעה
;
.
פייגנברג, אולג
;
.
רפאל, גינת
;
.
Volume :
1053
Co-Authors:
Levin, E., Dept. Postharvest Science, ARO, Volcani Center, Bet Dagan, Israel
Sela, N., Dept. Postharvest Science, ARO, Volcani Center, Bet Dagan, Israel
Raphael, G., Dept. Postharvest Science, ARO, Volcani Center, Bet Dagan, Israel
Feygenberg, O., Dept. Postharvest Science, ARO, Volcani Center, Bet Dagan, Israel
Droby, S., Dept. Postharvest Science, ARO, Volcani Center, Bet Dagan, Israel
Wisniewski, M., Appalachian Fruit Research Station, USDA-ARS 2217, Wiltshire Road, Kearneysville, WV, United States
Facilitators :
From page:
37
To page:
50
(
Total pages:
14
)
Abstract:
To gain better understanding of the molecular interactions between the yeast biocontrol agent Metschnikowia fructicola and citrus fruit and Penicillium digitatum, microarray analysis was performed on grapefruit surface wounds using an Affymetrix Citrus GeneChip in concert with transcriptomic analysis, using RNASeq technology. The data indicated that yeast application induced, among others, the expression of the genes encoding respiratory burst oxidase (Rbo), phenylalanine ammonia-lyase (PAL), chalcone synthase (CHS) and 4-coumarate-CoA ligase (4CL). In contrast, three genes, peroxidase (POD), superoxide dismutase (SOD) and catalase (CAT), were down-regulated in grapefruit peel tissue treated with yeast cells. Moreover, suppression was correlated with significantly higher levels of hydrogen peroxide, superoxide anion and hydroxyl radical production in yeast-treated surface wounds. Interestingly, large amounts of hydrogen peroxide were detected inside yeast cells recovered from wounded fruit tissue, indicating the ability of the yeast to activate reactive oxygen species when it is in contact with plant tissue. In the antagonist-pathogen interaction, genes related to transmembrane, multidrug transport and to amino acid metabolism were induced. In the antagonist-fruit interaction, expression of genes involved in oxidative stress, iron homeostasis, zinc homeostasis, and lipid metabolism were induced. This study provides the first global picture of gene expression changes in grapefruit in response to the yeast antagonist M. fructicola.
Note:
Related Files :
Citrus
Citrus x paradisi
Mechanism of action
Metschnikowia fructicola
microarray
Penicillium digitatum
Postharvest biocontrol
RNA-seq
ROS
עוד תגיות
תוכן קשור
More details
DOI :
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר מתוך כינוס
;
.
Language:
אנגלית
Editors' remarks:
ID:
25029
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:12
You may also be interested in
Scientific Publication
Molecular interactions between the biocontrol agent Metschnikowia fructicola and citrus fruit tissue and Penicillium digitatum
1053
Levin, E., Dept. Postharvest Science, ARO, Volcani Center, Bet Dagan, Israel
Sela, N., Dept. Postharvest Science, ARO, Volcani Center, Bet Dagan, Israel
Raphael, G., Dept. Postharvest Science, ARO, Volcani Center, Bet Dagan, Israel
Feygenberg, O., Dept. Postharvest Science, ARO, Volcani Center, Bet Dagan, Israel
Droby, S., Dept. Postharvest Science, ARO, Volcani Center, Bet Dagan, Israel
Wisniewski, M., Appalachian Fruit Research Station, USDA-ARS 2217, Wiltshire Road, Kearneysville, WV, United States
Molecular interactions between the biocontrol agent Metschnikowia fructicola and citrus fruit tissue and Penicillium digitatum
To gain better understanding of the molecular interactions between the yeast biocontrol agent Metschnikowia fructicola and citrus fruit and Penicillium digitatum, microarray analysis was performed on grapefruit surface wounds using an Affymetrix Citrus GeneChip in concert with transcriptomic analysis, using RNASeq technology. The data indicated that yeast application induced, among others, the expression of the genes encoding respiratory burst oxidase (Rbo), phenylalanine ammonia-lyase (PAL), chalcone synthase (CHS) and 4-coumarate-CoA ligase (4CL). In contrast, three genes, peroxidase (POD), superoxide dismutase (SOD) and catalase (CAT), were down-regulated in grapefruit peel tissue treated with yeast cells. Moreover, suppression was correlated with significantly higher levels of hydrogen peroxide, superoxide anion and hydroxyl radical production in yeast-treated surface wounds. Interestingly, large amounts of hydrogen peroxide were detected inside yeast cells recovered from wounded fruit tissue, indicating the ability of the yeast to activate reactive oxygen species when it is in contact with plant tissue. In the antagonist-pathogen interaction, genes related to transmembrane, multidrug transport and to amino acid metabolism were induced. In the antagonist-fruit interaction, expression of genes involved in oxidative stress, iron homeostasis, zinc homeostasis, and lipid metabolism were induced. This study provides the first global picture of gene expression changes in grapefruit in response to the yeast antagonist M. fructicola.
Scientific Publication
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