נגישות
menu      
חיפוש מתקדם
תחביר
חפש...
הספר "אוצר וולקני"
אודות
תנאי שימוש
ניהול
קהילה:
אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Protoplast-fusion-mediated transfer of organelles from Microcitrus into Citrus and regeneration of novel alloplasmic trees
Year:
1989
Source of publication :
Theoretical and Applied Genetics
Authors :
ורדי, עליזה
;
.
פרידמן, אהובה
;
.
Volume :
78
Co-Authors:
Vardi, A., Institute of Horticulture, Agricultural Research Organization, The Volcani Center, Bet Dagan, 50250, Israel
Arzee-Gonen, P., Department of Plant Genetics, The Weizman Institute of Science, Rehovot, 76100, Israel
Frydman-Shani, A., Institute of Horticulture, Agricultural Research Organization, The Volcani Center, Bet Dagan, 50250, Israel
Bleichman, S., Department of Plant Genetics, The Weizman Institute of Science, Rehovot, 76100, Israel
Galun, E., Department of Plant Genetics, The Weizman Institute of Science, Rehovot, 76100, Israel
Facilitators :
From page:
741
To page:
747
(
Total pages:
7
)
Abstract:
Iodoacetate-treated Citrus protoplasts from embryogenic nucellar calli of Sour orange (C. aurantium) or from Rough lemon (C. jambhiri) were fused with γ-irradiated protoplasts from a related genus, Microcitrus. The fused protoplasts were cultured to obtain colonies and micro-calli. Micro-calli derived from these two fusion combinations were isolated, propagated and differentiated into embryos, which subsequently regenerated trees having the morphology of Sour orange or Rough lemon. These intergeneric fusions resulted in mitochondria with novel DNA, indicating recombination between the chondriomes of Citrus and Microcitrus. Chloroplast DNA analyses of fusion-derived embryos indicated that they contained the chloroplasts of either fusion-partner or a mix of these chloroplasts. Later plastome analyses of leaves from fully differentiated plants showed that cybrids having Rough lemon morphology had either Rough lemon or Microcitrus chloroplast DNA, indicating complete sorting out of chloroplasts. Likewise, sorting out of Microcitrus chloroplasts was detected in a cybrid plant having Sour orange morphology. © 1989 Springer-Verlag.
Note:
Related Files :
Chloroplast DNA
Citrus
Cybrids
Microcitrus
mitochondrial DNA
עוד תגיות
תוכן קשור
More details
DOI :
10.1007/BF00262572
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
25044
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:12
You may also be interested in
Scientific Publication
Protoplast-fusion-mediated transfer of organelles from Microcitrus into Citrus and regeneration of novel alloplasmic trees
78
Vardi, A., Institute of Horticulture, Agricultural Research Organization, The Volcani Center, Bet Dagan, 50250, Israel
Arzee-Gonen, P., Department of Plant Genetics, The Weizman Institute of Science, Rehovot, 76100, Israel
Frydman-Shani, A., Institute of Horticulture, Agricultural Research Organization, The Volcani Center, Bet Dagan, 50250, Israel
Bleichman, S., Department of Plant Genetics, The Weizman Institute of Science, Rehovot, 76100, Israel
Galun, E., Department of Plant Genetics, The Weizman Institute of Science, Rehovot, 76100, Israel
Protoplast-fusion-mediated transfer of organelles from Microcitrus into Citrus and regeneration of novel alloplasmic trees
Iodoacetate-treated Citrus protoplasts from embryogenic nucellar calli of Sour orange (C. aurantium) or from Rough lemon (C. jambhiri) were fused with γ-irradiated protoplasts from a related genus, Microcitrus. The fused protoplasts were cultured to obtain colonies and micro-calli. Micro-calli derived from these two fusion combinations were isolated, propagated and differentiated into embryos, which subsequently regenerated trees having the morphology of Sour orange or Rough lemon. These intergeneric fusions resulted in mitochondria with novel DNA, indicating recombination between the chondriomes of Citrus and Microcitrus. Chloroplast DNA analyses of fusion-derived embryos indicated that they contained the chloroplasts of either fusion-partner or a mix of these chloroplasts. Later plastome analyses of leaves from fully differentiated plants showed that cybrids having Rough lemon morphology had either Rough lemon or Microcitrus chloroplast DNA, indicating complete sorting out of chloroplasts. Likewise, sorting out of Microcitrus chloroplasts was detected in a cybrid plant having Sour orange morphology. © 1989 Springer-Verlag.
Scientific Publication
You may also be interested in