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Larvicidal activities against agricultural pests of transgenic Escherichia coli expressing combinations of four genes from Bacillus thuringiensis
Year:
2007
Source of publication :
Archives of Microbiology
Authors :
הורוביץ, רמי
;
.
חסדן, ודים
;
.
ריפא, מריו
;
.
Volume :
188
Co-Authors:


Sapojnik, M., Department of Life Sciences, Ben-Gurion University of the Negev, P.O. Box 653, Be'er-Sheva 84105, Israel
Zaritsky, A., Department of Life Sciences, Ben-Gurion University of the Negev, P.O. Box 653, Be'er-Sheva 84105, Israel
Boussiba, S., Microalgal Biotechnology Laboratory, Blaustein Institute for Desert Research, Ben-Gurion University at Sede-Boker, Sede-Boker 84990, Israel
Manasherob, R., Department of Life Sciences, Ben-Gurion University of the Negev, P.O. Box 653, Be'er-Sheva 84105, Israel, Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305-5120, United States
Ben-Dov, E., Achva Academic College, MP Shikmim 79800, Israel, National Institute for Biotechnology in the Negev, Ben-Gurion University of the Negev, P.O. Box 653, Be'er-Sheva 84105, Israel

Facilitators :
From page:
643
To page:
653
(
Total pages:
11
)
Abstract:
The genes cry1Ac and cry1Ca from Bacillus thuringiensis subsps. kurstaki HD-73 and aizawai 4J4, respectively, encoding δ-endotoxins against lepidopteran larvae were isolated, cloned and expressed in Escherichia coli, with and without cyt1Aa (encoding cytolytic protein) and p20 (accessory protein) from subsp. israelensis. Nine combinations of the genes under control of an early T7, PA1 inducible promoter, produced the encoding proteins. Toxicities were examined against larvae of three major agricultural pests: Pectinophora gossypiella, Helicoverpa armigera and Spodoptera littoralis. The clones expressing cyt1Aa, with or without p20, were not toxic. The clone expressing cry1Ac (pBt-1A) was the most toxic to P. gossypiella (LC50 of 0.27 × 108 cells g-1). Clone pBt-1CA expressing cry1Ca and cry1Ac displayed the highest toxicity (LC50 of 0.12 × 108 cells ml-1) against S. littoralis. Clone pBt-1CARCy expressing all four genes (cry1Ca, cry1Ac, p20, cyt1Aa) in tandem exhibited the highest toxicity to H. armigera (LC50 of 0.16 × 108 cells ml-1). Cyt1Aa failed to raise the toxicity of these Cry toxins against P. gossypiella and S. littoralis but significantly enhanced toxicity against H. armigera. Two additional clones expressing either cry1Ac or cry1Ca under tandem promoters, PA1 and PpsbA (constitutive), displayed significantly higher toxicities (7.5- to 140-fold) than their counterparts with PA1 alone, reducing the LC50 values to below 107 cells ml-1. © 2007 Springer-Verlag.
Note:
Related Files :
Agriculture
Animals
gene expression
Hemolysin Proteins
Lepidoptera
microbial pest control
עוד תגיות
תוכן קשור
More details
DOI :
10.1007/s00203-007-0285-y
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
25047
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:12
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Scientific Publication
Larvicidal activities against agricultural pests of transgenic Escherichia coli expressing combinations of four genes from Bacillus thuringiensis
188


Sapojnik, M., Department of Life Sciences, Ben-Gurion University of the Negev, P.O. Box 653, Be'er-Sheva 84105, Israel
Zaritsky, A., Department of Life Sciences, Ben-Gurion University of the Negev, P.O. Box 653, Be'er-Sheva 84105, Israel
Boussiba, S., Microalgal Biotechnology Laboratory, Blaustein Institute for Desert Research, Ben-Gurion University at Sede-Boker, Sede-Boker 84990, Israel
Manasherob, R., Department of Life Sciences, Ben-Gurion University of the Negev, P.O. Box 653, Be'er-Sheva 84105, Israel, Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305-5120, United States
Ben-Dov, E., Achva Academic College, MP Shikmim 79800, Israel, National Institute for Biotechnology in the Negev, Ben-Gurion University of the Negev, P.O. Box 653, Be'er-Sheva 84105, Israel

Larvicidal activities against agricultural pests of transgenic Escherichia coli expressing combinations of four genes from Bacillus thuringiensis
The genes cry1Ac and cry1Ca from Bacillus thuringiensis subsps. kurstaki HD-73 and aizawai 4J4, respectively, encoding δ-endotoxins against lepidopteran larvae were isolated, cloned and expressed in Escherichia coli, with and without cyt1Aa (encoding cytolytic protein) and p20 (accessory protein) from subsp. israelensis. Nine combinations of the genes under control of an early T7, PA1 inducible promoter, produced the encoding proteins. Toxicities were examined against larvae of three major agricultural pests: Pectinophora gossypiella, Helicoverpa armigera and Spodoptera littoralis. The clones expressing cyt1Aa, with or without p20, were not toxic. The clone expressing cry1Ac (pBt-1A) was the most toxic to P. gossypiella (LC50 of 0.27 × 108 cells g-1). Clone pBt-1CA expressing cry1Ca and cry1Ac displayed the highest toxicity (LC50 of 0.12 × 108 cells ml-1) against S. littoralis. Clone pBt-1CARCy expressing all four genes (cry1Ca, cry1Ac, p20, cyt1Aa) in tandem exhibited the highest toxicity to H. armigera (LC50 of 0.16 × 108 cells ml-1). Cyt1Aa failed to raise the toxicity of these Cry toxins against P. gossypiella and S. littoralis but significantly enhanced toxicity against H. armigera. Two additional clones expressing either cry1Ac or cry1Ca under tandem promoters, PA1 and PpsbA (constitutive), displayed significantly higher toxicities (7.5- to 140-fold) than their counterparts with PA1 alone, reducing the LC50 values to below 107 cells ml-1. © 2007 Springer-Verlag.
Scientific Publication
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