אסיף מאגר המחקר החקלאי

The first confirmed selenoprotein in the plant kingdom: MALDI-QqTOF mass mapping and MS/MS measurements show that a UGA 'stop' codon determines a selenocysteine residue in a Chlamydomonas reinhardtii glutaihione peroxidase homologue

Year:

2002

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Co-Authors:

She, Y.-M., Dept. of Physics, University of Manitoba, Winnipeg, Man. R3T 2N2, Canada, Research Institute, Hospital for Sick Children, Toronto, Ont. M5G 1X8, Canada
Fu, L.-H., Dept. of Fruit-Tree Breeding, Volcani Center, Bet Dagan, Israel
Wang, X.-F., Dept. of Fruit-Tree Breeding, Volcani Center, Bet Dagan, Israel
Eyal, Y., Dept. of Fruit-Tree Breeding, Volcani Center, Bet Dagan, Israel
Donald, L., Dept. of Chemistry, University of Manitoba, Winnipeg, Man. R3T 2N2, Canada
Spicer, V., Dept. of Physics, University of Manitoba, Winnipeg, Man. R3T 2N2, Canada
Ens, W., Dept. of Physics, University of Manitoba, Winnipeg, Man. R3T 2N2, Canada
Ben-Hayyim, G., Dept. of Fruit-Tree Breeding, Volcani Center, Bet Dagan, Israel
Standing, K.G., Dept. of Physics, University of Manitoba, Winnipeg, Man. R3T 2N2, Canada

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Abstract:

The first confirmed selenoprotein in the plant kingdom was studied. The mass of the whole protein was measured by MALDI on the Manitoba QqTOf instrument. The crucial codon UGA was found in the nucleic acid sequence, which predicted a protein of 201 amino acid residues. The b18 ion had a normal isotopic distribution, whereas the distribution for b19 was anomalous. It is observed that both the overall mass measurements and the MS/MS data indicated truncation at the N-terminus, most commonly a deletion of the first 27 residues.

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