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פותח על ידי קלירמאש פתרונות בע"מ -
Effects of ethylene degreening on the transcriptome of mandarin flesh
Year:
2011
Source of publication :
Postharvest Biology and Technology
Authors :
מיוני-קירשנבאום, לינה
;
.
פורת, רון
;
.
Volume :
60
Co-Authors:
Mayuoni, L., Department of Postharvest Science of Fresh Produce, ARO, the Volcani Center, P.O. Box 6, Bet-Dagan 50250, Israel
Sharabi-Schwager, M., Department of Postharvest Science of Fresh Produce, ARO, the Volcani Center, P.O. Box 6, Bet-Dagan 50250, Israel
Feldmesser, E., Department of Biological Services, Weizmann Institute of Science, P.O. Box 26, Rehovot 76100, Israel
Porat, R., Department of Postharvest Science of Fresh Produce, ARO, the Volcani Center, P.O. Box 6, Bet-Dagan 50250, Israel
Facilitators :
From page:
75
To page:
82
(
Total pages:
8
)
Abstract:
The commercial practice of degreening citrus fruit by exposure to ethylene was developed to accelerate peel color development, but little is yet known about its putative effects on the edible flesh. To improve our understanding of the molecular mechanisms involved in the responses of mandarin flesh to ethylene degreening, we performed genome-wide transcriptional profiling analysis with the Affymetrix Citrus GeneChip ®. Overall, out of 30,171 probe sets representing citrus transcripts on the microarray, we found that expression of 734 probe sets was significantly (q≤0.01) altered by factors of at least 3 by exposure to air or 4μLL -1 ethylene for 48h at 20°C. One-way ANOVA pair-wise comparisons revealed 163 probe sets that were affected by exposure both to air and to ethylene, 498 sets that were affected only by ethylene, and 73 that were affected only by air. Overall, keeping the fruit at 20°C for 48h without ethylene led to an arrest of general cellular and metabolic activity. In contrast, exposure to ethylene stimulated adaptation processes that involved induction of gene expression related to carbohydrate, amino acid, secondary and hormone metabolism, stress and defense, and activation of regulatory processes, including transcription regulation and protein posttranslational modifications. In light of these observations, we propose that ethylene degreening simultaneously stimulates two independent processes in mandarin flesh: on the one hand, storing the fruit at a relatively high temperature of 20°C resulted in suppression of gene expression and overall metabolic arrest whereas, on the other hand, exposure to ethylene activated gene expression and stimulated various adaptation and metabolic processes, which might impact on fruit internal and nutritional quality. © 2010 Elsevier B.V.
Note:
Related Files :
Citrus
Degreening
ethylene
Mandarin
transcriptome
עוד תגיות
תוכן קשור
More details
DOI :
10.1016/j.postharvbio.2010.11.009
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
25104
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:12
Scientific Publication
Effects of ethylene degreening on the transcriptome of mandarin flesh
60
Mayuoni, L., Department of Postharvest Science of Fresh Produce, ARO, the Volcani Center, P.O. Box 6, Bet-Dagan 50250, Israel
Sharabi-Schwager, M., Department of Postharvest Science of Fresh Produce, ARO, the Volcani Center, P.O. Box 6, Bet-Dagan 50250, Israel
Feldmesser, E., Department of Biological Services, Weizmann Institute of Science, P.O. Box 26, Rehovot 76100, Israel
Porat, R., Department of Postharvest Science of Fresh Produce, ARO, the Volcani Center, P.O. Box 6, Bet-Dagan 50250, Israel
Effects of ethylene degreening on the transcriptome of mandarin flesh
The commercial practice of degreening citrus fruit by exposure to ethylene was developed to accelerate peel color development, but little is yet known about its putative effects on the edible flesh. To improve our understanding of the molecular mechanisms involved in the responses of mandarin flesh to ethylene degreening, we performed genome-wide transcriptional profiling analysis with the Affymetrix Citrus GeneChip ®. Overall, out of 30,171 probe sets representing citrus transcripts on the microarray, we found that expression of 734 probe sets was significantly (q≤0.01) altered by factors of at least 3 by exposure to air or 4μLL -1 ethylene for 48h at 20°C. One-way ANOVA pair-wise comparisons revealed 163 probe sets that were affected by exposure both to air and to ethylene, 498 sets that were affected only by ethylene, and 73 that were affected only by air. Overall, keeping the fruit at 20°C for 48h without ethylene led to an arrest of general cellular and metabolic activity. In contrast, exposure to ethylene stimulated adaptation processes that involved induction of gene expression related to carbohydrate, amino acid, secondary and hormone metabolism, stress and defense, and activation of regulatory processes, including transcription regulation and protein posttranslational modifications. In light of these observations, we propose that ethylene degreening simultaneously stimulates two independent processes in mandarin flesh: on the one hand, storing the fruit at a relatively high temperature of 20°C resulted in suppression of gene expression and overall metabolic arrest whereas, on the other hand, exposure to ethylene activated gene expression and stimulated various adaptation and metabolic processes, which might impact on fruit internal and nutritional quality. © 2010 Elsevier B.V.
Scientific Publication
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