נגישות
menu      
חיפוש מתקדם
תחביר
חפש...
הספר "אוצר וולקני"
אודות
תנאי שימוש
ניהול
קהילה:
אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
The relation between isozymes of β-1,3-giucanase and resistance of near-isogenic maize inbred lines to exserohilum turcicum
Year:
1992
Authors :
ראובני, ראובן
;
.
שמעוני, משה
;
.
Volume :
14
Co-Authors:
Shimoni, M., Department of Plant Pathology, NeweYa’ar Experiment Station, Haifa, 31999, Israel
Bar-Zur, A., Department of Field Crops, ARO, NeweYa’ar Experiment Station, Haifa, 31999, Israel
Reuveni, R., Department of Plant Pathology, NeweYa’ar Experiment Station, Haifa, 31999, Israel
Facilitators :
From page:
285
To page:
288
(
Total pages:
4
)
Abstract:
β-1,3-gIucanase isozymes in leaves of maize susceptible or resistant to Exserohilum turcicum were visualized using native polyacrylamide gel electrophoresis and staining with 2,3,5-triphenyltetrazolium chloride. In noninoculated leaves, 2 bands (B, C) with low intensity were observed in the susceptible cultivar Jubilee (Rfs 0.74, 0.77). In the susceptible line B37, one intense band (C) appeared (Rf = 0.77). Bands with Rfs of 0.74 and 0.77 were absent from the resistant isoline B37HtN, but two other bands (A, E) were detected (Rfs 0.70, 0.87). After inoculation of the three maize genotypes with E. turcicum, an intensification of all bands was detected in extracts prepared from the symptomless areas of inoculated leaves. In inoculated plants of line B37HtN bands B and C, which were absent from noninoculated leaves, were detected, as well as an additional isozyme (D, Rf 0.83), Infection of leaves with E. turcicum significantly increased total activity of β-1,3-glucanase in leaf extracts from the resistant line B37HtN but not from the susceptible line B37. Differences between resistant and susceptible lines in patterns of isozymes of β- L,3-glucanase before inoculation and in total activity of the enzyme after inoculation may be useful in rapid detection of resistance in maize loE. turcicum. © Taylor & Francis Group, LLC.
Note:
Related Files :
עוד תגיות
תוכן קשור
More details
DOI :
10.1080/07060669209500865
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
25137
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:12
You may also be interested in
Scientific Publication
The relation between isozymes of β-1,3-giucanase and resistance of near-isogenic maize inbred lines to exserohilum turcicum
14
Shimoni, M., Department of Plant Pathology, NeweYa’ar Experiment Station, Haifa, 31999, Israel
Bar-Zur, A., Department of Field Crops, ARO, NeweYa’ar Experiment Station, Haifa, 31999, Israel
Reuveni, R., Department of Plant Pathology, NeweYa’ar Experiment Station, Haifa, 31999, Israel
The relation between isozymes of β-1,3-giucanase and resistance of near-isogenic maize inbred lines to exserohilum turcicum
β-1,3-gIucanase isozymes in leaves of maize susceptible or resistant to Exserohilum turcicum were visualized using native polyacrylamide gel electrophoresis and staining with 2,3,5-triphenyltetrazolium chloride. In noninoculated leaves, 2 bands (B, C) with low intensity were observed in the susceptible cultivar Jubilee (Rfs 0.74, 0.77). In the susceptible line B37, one intense band (C) appeared (Rf = 0.77). Bands with Rfs of 0.74 and 0.77 were absent from the resistant isoline B37HtN, but two other bands (A, E) were detected (Rfs 0.70, 0.87). After inoculation of the three maize genotypes with E. turcicum, an intensification of all bands was detected in extracts prepared from the symptomless areas of inoculated leaves. In inoculated plants of line B37HtN bands B and C, which were absent from noninoculated leaves, were detected, as well as an additional isozyme (D, Rf 0.83), Infection of leaves with E. turcicum significantly increased total activity of β-1,3-glucanase in leaf extracts from the resistant line B37HtN but not from the susceptible line B37. Differences between resistant and susceptible lines in patterns of isozymes of β- L,3-glucanase before inoculation and in total activity of the enzyme after inoculation may be useful in rapid detection of resistance in maize loE. turcicum. © Taylor & Francis Group, LLC.
Scientific Publication
You may also be interested in