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פותח על ידי קלירמאש פתרונות בע"מ -
Differences in neuronal differentiation between the transient cranial (Frorieps') and normal dorsal root ganglia
Year:
2002
Source of publication :
Developmental Brain Research
Authors :
גורן, שלמה
;
.
Volume :
135
Co-Authors:
Avivi, C., Faculty of Life Sciences, Gonda Research Center, Bar-Ilan University, 52900 Ramat-Gan, Israel
Cui, S., College of Biology, China Agricultural University, Beijing 10094, China
Goren, S., Faculty of Life Sciences, Gonda Research Center, Bar-Ilan University, 52900 Ramat-Gan, Israel
Goldstein, R.S., Faculty of Life Sciences, Gonda Research Center, Bar-Ilan University, 52900 Ramat-Gan, Israel
Facilitators :
From page:
19
To page:
28
(
Total pages:
10
)
Abstract:
The Frorieps' ganglia are dorsal root ganglia (DRG) that form, grow slowly compared to normal DRG, and then degenerate during normal embryonic development of amniotes. Their fate has been shown to be regulated by the Hox family and other genes involved in pattern formation, and by the mesodermal microenvironment of the cranial somites in which they develop. Neurons are known to differentiate within the Frorieps' DRG in the course of their short life span. In this study, we compared several aspects of neural differentiation between the longest-lived Frorieps' ganglia, DRG2, and normal trunk DRG in chick embryos. The expression of transcription factor Islet-1 and the RNA-binding protein Hu differ between normal and Frorieps' DRG at St. 23 (embryonic day 4). Islet-1 is expressed in a higher proportion of cells in DRG2 than DRG5, suggesting that cells in DRG2 differentiate more rapidly into neurons than in normal DRG. This molecular difference appears at the same developmental stage as overt morphological differences between DRG2 and normal DRG. Other LIM-homeobox proteins (Lim-1, -2 and -3 and Islet-2) are not expressed either in normal or Frorieps' DRG at early stages of development. Somite-grafting experiments reveal that the increased proportion of Isl-1+ in DRG2 is due to the special microenvironment of the cranial somites. In contrast to Islet-1, the Hu antigen is expressed in a slightly lower proportion of cells in DRG2 at St. 23. At St. 28, there is a significant population of neurons in DRG2 that are Isl-1+/Hu-. Since Islet-1 is normally expressed before Hu in DRG cells, it is possible that the reduced growth rate of the DRG2 ganglion may partially result from the inability of precociously differentiating Islet-1+ neurons to further mature, as reflected by Hu antigen expression. In contrast to the neuronal markers examined, the satellite cell marker 7B3 is expressed at similar levels in DRG2 and DRG5. © Elsevier Science B.V. All rights reserved.
Note:
Related Files :
Animals
cranial nerve
gene expression
Genes, Homeobox
microenvironment
Neurodegeneration
RNA
somite
spinal ganglion
עוד תגיות
תוכן קשור
More details
DOI :
10.1016/S0165-3806(02)00272-9
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
25231
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:13
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Scientific Publication
Differences in neuronal differentiation between the transient cranial (Frorieps') and normal dorsal root ganglia
135
Avivi, C., Faculty of Life Sciences, Gonda Research Center, Bar-Ilan University, 52900 Ramat-Gan, Israel
Cui, S., College of Biology, China Agricultural University, Beijing 10094, China
Goren, S., Faculty of Life Sciences, Gonda Research Center, Bar-Ilan University, 52900 Ramat-Gan, Israel
Goldstein, R.S., Faculty of Life Sciences, Gonda Research Center, Bar-Ilan University, 52900 Ramat-Gan, Israel
Differences in neuronal differentiation between the transient cranial (Frorieps') and normal dorsal root ganglia
The Frorieps' ganglia are dorsal root ganglia (DRG) that form, grow slowly compared to normal DRG, and then degenerate during normal embryonic development of amniotes. Their fate has been shown to be regulated by the Hox family and other genes involved in pattern formation, and by the mesodermal microenvironment of the cranial somites in which they develop. Neurons are known to differentiate within the Frorieps' DRG in the course of their short life span. In this study, we compared several aspects of neural differentiation between the longest-lived Frorieps' ganglia, DRG2, and normal trunk DRG in chick embryos. The expression of transcription factor Islet-1 and the RNA-binding protein Hu differ between normal and Frorieps' DRG at St. 23 (embryonic day 4). Islet-1 is expressed in a higher proportion of cells in DRG2 than DRG5, suggesting that cells in DRG2 differentiate more rapidly into neurons than in normal DRG. This molecular difference appears at the same developmental stage as overt morphological differences between DRG2 and normal DRG. Other LIM-homeobox proteins (Lim-1, -2 and -3 and Islet-2) are not expressed either in normal or Frorieps' DRG at early stages of development. Somite-grafting experiments reveal that the increased proportion of Isl-1+ in DRG2 is due to the special microenvironment of the cranial somites. In contrast to Islet-1, the Hu antigen is expressed in a slightly lower proportion of cells in DRG2 at St. 23. At St. 28, there is a significant population of neurons in DRG2 that are Isl-1+/Hu-. Since Islet-1 is normally expressed before Hu in DRG cells, it is possible that the reduced growth rate of the DRG2 ganglion may partially result from the inability of precociously differentiating Islet-1+ neurons to further mature, as reflected by Hu antigen expression. In contrast to the neuronal markers examined, the satellite cell marker 7B3 is expressed at similar levels in DRG2 and DRG5. © Elsevier Science B.V. All rights reserved.
Scientific Publication
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