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פותח על ידי קלירמאש פתרונות בע"מ -
Regulation of osteopontin gene expression during egg shell formation in the laying hen by mechanical strain
Year:
1998
Source of publication :
Matrix Biology
Authors :
בן-בסט, שגיא
;
.
בר, אריה
;
.
ירדן, נועה
;
.
לבלין, אירינה
;
.
פינס, מרק
;
.
Volume :
17
Co-Authors:
Lavelin, I., Institute of Animal Science, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Yarden, N., Institute of Animal Science, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Ben-Bassat, S., Institute of Animal Science, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Bar, A., Institute of Animal Science, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Pines, M., Institute of Animal Science, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel, Institute of Animal Science, ARO, Volcani Center, P.O.Box 6, Bet Dagan 50250, Israel
Facilitators :
From page:
615
To page:
623
(
Total pages:
9
)
Abstract:
The aim of this study is to evaluate the regulation of the osteopontin (OPN) gene expression by non-hormonal stimuli, such as calcium flux and mechanical strain during the daily egg cycle in the oviduct of the laying hen. After the egg enters the eggshell gland (ESG), the OPN gene is expressed by the epithelium cells in two waves: first by the basal cells and only then by the apical cells of the epithelium. A reduction in OPN gene expression was observed 1 h prior to laying. The calbindin gene, which marks the onset of calcification, was found to be expressed in the glandular epithelium starting 2 h after OPN gene expression. In addition, the formation of soft shells was accompanied by a reduction in calbindin, but not in OPN, gene expression. The application of a mechanical strain comparable to that induced by an egg led to induction of OPN gene expression at a normally quiescent phase in the cyclical expression of this gene. The induction of the gene was time- and strain-dependent and temporally similar to that induced by the entry of the egg into the ESG. In contrast, the calbindin gene was not affected by mechanical strain. The ESG of the laying hen provides a system to study the effect of a mechanical strain on matrix protein production in vivo, in a relevant physiological setting. The finding suggests that, in contrast to calbindin, OPN gene expression is not regulated by calcium flux but rather by the mechanical strain imposed by the resident egg.
Note:
Related Files :
animal experiment
Animals
animal tissue
Chickens
Egg Shell
epithelium cell
Female
gene expression regulation
עוד תגיות
תוכן קשור
More details
DOI :
10.1016/S0945-053X(98)90112-3
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
25261
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:13
Scientific Publication
Regulation of osteopontin gene expression during egg shell formation in the laying hen by mechanical strain
17
Lavelin, I., Institute of Animal Science, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Yarden, N., Institute of Animal Science, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Ben-Bassat, S., Institute of Animal Science, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Bar, A., Institute of Animal Science, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Pines, M., Institute of Animal Science, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel, Institute of Animal Science, ARO, Volcani Center, P.O.Box 6, Bet Dagan 50250, Israel
Regulation of osteopontin gene expression during egg shell formation in the laying hen by mechanical strain
The aim of this study is to evaluate the regulation of the osteopontin (OPN) gene expression by non-hormonal stimuli, such as calcium flux and mechanical strain during the daily egg cycle in the oviduct of the laying hen. After the egg enters the eggshell gland (ESG), the OPN gene is expressed by the epithelium cells in two waves: first by the basal cells and only then by the apical cells of the epithelium. A reduction in OPN gene expression was observed 1 h prior to laying. The calbindin gene, which marks the onset of calcification, was found to be expressed in the glandular epithelium starting 2 h after OPN gene expression. In addition, the formation of soft shells was accompanied by a reduction in calbindin, but not in OPN, gene expression. The application of a mechanical strain comparable to that induced by an egg led to induction of OPN gene expression at a normally quiescent phase in the cyclical expression of this gene. The induction of the gene was time- and strain-dependent and temporally similar to that induced by the entry of the egg into the ESG. In contrast, the calbindin gene was not affected by mechanical strain. The ESG of the laying hen provides a system to study the effect of a mechanical strain on matrix protein production in vivo, in a relevant physiological setting. The finding suggests that, in contrast to calbindin, OPN gene expression is not regulated by calcium flux but rather by the mechanical strain imposed by the resident egg.
Scientific Publication
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