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Reduction of benzenoid synthesis in petunia flowers reveals multiple pathways to benzoic acid and enhancement in auxin transport
Year:
2006
Source of publication :
Plant Cell
Authors :
פרידמן, איל
;
.
Volume :
18
Co-Authors:
Orlova, I., Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47907, United States
Marshall-Colón, A., Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47907, United States
Schnepp, J., Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47907, United States
Wood, B., Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47907, United States
Varbanova, M., Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, United States
Fridman, E., Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, United States
Blakeslee, J.J., Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47907, United States
Peer, W.A., Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47907, United States
Murphy, A.S., Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47907, United States
Rhodes, D., Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47907, United States
Pichersky, E., Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, United States
Dudareva, N., Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47907, United States
Facilitators :
From page:
3458
To page:
3475
(
Total pages:
18
)
Abstract:
In plants, benzoic acid (BA) is believed to be synthesized from Phe through shortening of the propyl side chain by two carbons. It is hypothesized that this chain shortening occurs via either a β-oxidative or non-β-oxidative pathway. Previous in vivo isotope labeling and metabolic flux analysis of the benzenoid network in petunia (Petunia hybrida) flowers revealed that both pathways yield benzenoid compounds and that benzylbenzoate is an intermediate between L-Phe and BA. To test this hypothesis, we generated transgenic petunia plants in which the expression of BPBT, the gene encoding the enzyme that uses benzoyl-CoA and benzyl alcohol to make benzylbenzoate, was reduced or eliminated. Elimination of benzylbenzoate formation decreased the endogenous pool of BA and methylbenzoate emission but increased emission of benzyl alcohol and benzylaldehyde, confirming the contribution of benzylbenzoate to BA formation. Labeling experiments with 2H5-PHe revealed a dilution of isotopic abundance in most measured compounds in the dark, suggesting an alternative pathway from a precursor other than Phe, possibly phenylpyruvate. Suppression of BPBT activity also affected the overall morphology of petunia plants, resulting in larger flowers and leaves, thicker stems, and longer internodes, which was consistent with the increased auxin transport in transgenic plants. This suggests that BPBT is involved in metabolic processes in vegetative tissues as well. © 2006 American Society of Plant Biologists.
Note:
Related Files :
biosynthesis
computer simulation
Genetics
light
molecular genetics
Petunia
phenotype
Plants
עוד תגיות
תוכן קשור
More details
DOI :
10.1105/tpc.106.046227
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
25263
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:13
You may also be interested in
Scientific Publication
Reduction of benzenoid synthesis in petunia flowers reveals multiple pathways to benzoic acid and enhancement in auxin transport
18
Orlova, I., Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47907, United States
Marshall-Colón, A., Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47907, United States
Schnepp, J., Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47907, United States
Wood, B., Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47907, United States
Varbanova, M., Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, United States
Fridman, E., Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, United States
Blakeslee, J.J., Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47907, United States
Peer, W.A., Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47907, United States
Murphy, A.S., Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47907, United States
Rhodes, D., Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47907, United States
Pichersky, E., Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, United States
Dudareva, N., Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47907, United States
Reduction of benzenoid synthesis in petunia flowers reveals multiple pathways to benzoic acid and enhancement in auxin transport
In plants, benzoic acid (BA) is believed to be synthesized from Phe through shortening of the propyl side chain by two carbons. It is hypothesized that this chain shortening occurs via either a β-oxidative or non-β-oxidative pathway. Previous in vivo isotope labeling and metabolic flux analysis of the benzenoid network in petunia (Petunia hybrida) flowers revealed that both pathways yield benzenoid compounds and that benzylbenzoate is an intermediate between L-Phe and BA. To test this hypothesis, we generated transgenic petunia plants in which the expression of BPBT, the gene encoding the enzyme that uses benzoyl-CoA and benzyl alcohol to make benzylbenzoate, was reduced or eliminated. Elimination of benzylbenzoate formation decreased the endogenous pool of BA and methylbenzoate emission but increased emission of benzyl alcohol and benzylaldehyde, confirming the contribution of benzylbenzoate to BA formation. Labeling experiments with 2H5-PHe revealed a dilution of isotopic abundance in most measured compounds in the dark, suggesting an alternative pathway from a precursor other than Phe, possibly phenylpyruvate. Suppression of BPBT activity also affected the overall morphology of petunia plants, resulting in larger flowers and leaves, thicker stems, and longer internodes, which was consistent with the increased auxin transport in transgenic plants. This suggests that BPBT is involved in metabolic processes in vegetative tissues as well. © 2006 American Society of Plant Biologists.
Scientific Publication
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