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פותח על ידי קלירמאש פתרונות בע"מ -
Volatile ester formation in roses. Identification of an acetyl-coenzyme A. Geraniol/citronellol acetyltransferase in developing rose petals
Year:
2003
Authors :
בר, עינת
;
.
וולפין, חנה
;
.
לוינסון, אפרים
;
.
שליט, משה
;
.
תמרי, טל
;
.
Volume :
131
Co-Authors:
Shalit, M., Department of Vegetable Crops, Newe Ya'ar Research Center, Agricultural Research Organization, P.O. Box 1021, Ramat Yishay 30095, Israel, Inst. of Plant Sci./Genet. in Agric., Fac. Agric., Food,/Environ. Qual. S., Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Guterman, I., Inst. of Plant Sci./Genet. in Agric., Fac. Agric., Food,/Environ. Qual. S., Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Volpin, H., Department of Genomics, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Bar, E., Department of Vegetable Crops, Newe Ya'ar Research Center, Agricultural Research Organization, P.O. Box 1021, Ramat Yishay 30095, Israel
Tamari, T., Department of Vegetable Crops, Newe Ya'ar Research Center, Agricultural Research Organization, P.O. Box 1021, Ramat Yishay 30095, Israel
Menda, N., Inst. of Plant Sci./Genet. in Agric., Fac. Agric., Food,/Environ. Qual. S., Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Adam, Z., Inst. of Plant Sci./Genet. in Agric., Fac. Agric., Food,/Environ. Qual. S., Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Zamir, D., Inst. of Plant Sci./Genet. in Agric., Fac. Agric., Food,/Environ. Qual. S., Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Vainstein, A., Inst. of Plant Sci./Genet. in Agric., Fac. Agric., Food,/Environ. Qual. S., Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Weiss, D., Inst. of Plant Sci./Genet. in Agric., Fac. Agric., Food,/Environ. Qual. S., Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Pichersky, E., Dept. Molec., Cell.,/Devmtl. Biol., University of Michigan, 830 North University Street, Ann Arbor, MI 48109-1048, United States
Lewinsohn, E., Department of Vegetable Crops, Newe Ya'ar Research Center, Agricultural Research Organization, P.O. Box 1021, Ramat Yishay 30095, Israel
Facilitators :
From page:
1868
To page:
1876
(
Total pages:
9
)
Abstract:
The aroma of roses (Rosa hybrida) is due to more than 400 volatile compounds including terpenes, esters, and phenolic derivatives. 2-Phenylethyl acetate, cis-3-hexenyl acetate, geranyl acetate, and citronellyl acetate were identified as the main volatile esters emitted by the flowers of the scented rose var. "Fragrant Cloud." Cell-free extracts of petals acetylated several alcohols, utilizing acetyl-coenzyme A, to produce the corresponding acetate esters. Screening for genes similar to known plant alcohol acetyltransferases in a rose expressed sequence tag database yielded a cDNA (RhAAT1) encoding a protein with high similarity to several members of the BAHD family of acyltransferases. This cDNA was functionally expressed in Escherichia coli, and its gene product displayed acetyl-coenzyme A:geraniol acetyltransferase enzymatic activity in vitro. The RhAAT1 protein accepted other alcohols such as citronellol and 1-octanol as substrates, but 2-phenylethyl alcohol and cis-3-hexen-1-ol were poor substrates, suggesting that additional acetyltransferases are present in rose petals. The RhAAT1 protein is a polypeptide of 458 amino acids, with a calculated molecular mass of 51.8 kD, pI of 5.45, and is active as a monomer. The RhAAT1 gene was expressed exclusively in floral tissue with maximum transcript levels occurring at stage 4 of flower development, where scent emission is at its peak.
Note:
Related Files :
Acetyltransferases
Genes
Molecular structure
odors
Oils, Volatile
proteins
Rosa
terpenes
עוד תגיות
תוכן קשור
More details
DOI :
10.1104/pp.102.018572
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
25277
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:13
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Scientific Publication
Volatile ester formation in roses. Identification of an acetyl-coenzyme A. Geraniol/citronellol acetyltransferase in developing rose petals
131
Shalit, M., Department of Vegetable Crops, Newe Ya'ar Research Center, Agricultural Research Organization, P.O. Box 1021, Ramat Yishay 30095, Israel, Inst. of Plant Sci./Genet. in Agric., Fac. Agric., Food,/Environ. Qual. S., Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Guterman, I., Inst. of Plant Sci./Genet. in Agric., Fac. Agric., Food,/Environ. Qual. S., Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Volpin, H., Department of Genomics, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Bar, E., Department of Vegetable Crops, Newe Ya'ar Research Center, Agricultural Research Organization, P.O. Box 1021, Ramat Yishay 30095, Israel
Tamari, T., Department of Vegetable Crops, Newe Ya'ar Research Center, Agricultural Research Organization, P.O. Box 1021, Ramat Yishay 30095, Israel
Menda, N., Inst. of Plant Sci./Genet. in Agric., Fac. Agric., Food,/Environ. Qual. S., Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Adam, Z., Inst. of Plant Sci./Genet. in Agric., Fac. Agric., Food,/Environ. Qual. S., Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Zamir, D., Inst. of Plant Sci./Genet. in Agric., Fac. Agric., Food,/Environ. Qual. S., Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Vainstein, A., Inst. of Plant Sci./Genet. in Agric., Fac. Agric., Food,/Environ. Qual. S., Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Weiss, D., Inst. of Plant Sci./Genet. in Agric., Fac. Agric., Food,/Environ. Qual. S., Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Pichersky, E., Dept. Molec., Cell.,/Devmtl. Biol., University of Michigan, 830 North University Street, Ann Arbor, MI 48109-1048, United States
Lewinsohn, E., Department of Vegetable Crops, Newe Ya'ar Research Center, Agricultural Research Organization, P.O. Box 1021, Ramat Yishay 30095, Israel
Volatile ester formation in roses. Identification of an acetyl-coenzyme A. Geraniol/citronellol acetyltransferase in developing rose petals
The aroma of roses (Rosa hybrida) is due to more than 400 volatile compounds including terpenes, esters, and phenolic derivatives. 2-Phenylethyl acetate, cis-3-hexenyl acetate, geranyl acetate, and citronellyl acetate were identified as the main volatile esters emitted by the flowers of the scented rose var. "Fragrant Cloud." Cell-free extracts of petals acetylated several alcohols, utilizing acetyl-coenzyme A, to produce the corresponding acetate esters. Screening for genes similar to known plant alcohol acetyltransferases in a rose expressed sequence tag database yielded a cDNA (RhAAT1) encoding a protein with high similarity to several members of the BAHD family of acyltransferases. This cDNA was functionally expressed in Escherichia coli, and its gene product displayed acetyl-coenzyme A:geraniol acetyltransferase enzymatic activity in vitro. The RhAAT1 protein accepted other alcohols such as citronellol and 1-octanol as substrates, but 2-phenylethyl alcohol and cis-3-hexen-1-ol were poor substrates, suggesting that additional acetyltransferases are present in rose petals. The RhAAT1 protein is a polypeptide of 458 amino acids, with a calculated molecular mass of 51.8 kD, pI of 5.45, and is active as a monomer. The RhAAT1 gene was expressed exclusively in floral tissue with maximum transcript levels occurring at stage 4 of flower development, where scent emission is at its peak.
Scientific Publication
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