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אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Development of malaria parasites in mosquitoes fed with ookinetes suspended in defined media
Year:
1990
Source of publication :
Experientia
Authors :
סמיש, מיכאל
;
.
Volume :
46
Co-Authors:
Samish, M., Department of Entomology and Economic Zoology, Cook College, Rutgers University, P.O. Box 231, New Brunswick, 08903, New Jersey, United States
Facilitators :
From page:
224
To page:
225
(
Total pages:
2
)
Abstract:
Information concerning the specific nutritional requirements of malarial parasites developing in the mosquito host has been difficult to obtain, owing primarily to the complex nature of the blood meal that accompanies the parasites and the lack of success in culturing the complete invertebrate cycle of Plasmodium in vitro. The present report describes a blood-free system for infecting mosquitoes with ookinetes of Plasmodium berghei and for allowing the latter to develop into infective sporozoites. Ookinetes cultured in vitro were separated from blood proteins, suspended in defined medium, and fed to Anopheles stephensi mosquitoes through a membrane. The mosquitoes were then maintained on the same defined medium plus 5% sucrose. Infectivity of the parasites was demonstrated 17-19 days later by intracardial inoculation of the macerated mosquitoes into hamsters. This system makes it possible to evaluate nutritional factors that affect parasite development in the mosquito host under controlled conditions. © 1990 Birkhäuser Verlag Basel.
Note:
Related Files :
Animal
Cricetinae
in vitro development of Plasmodium
malaria
Plasmodium berghei
transmission of malaria
עוד תגיות
תוכן קשור
More details
DOI :
10.1007/BF02027323
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
25321
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:14
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Scientific Publication
Development of malaria parasites in mosquitoes fed with ookinetes suspended in defined media
46
Samish, M., Department of Entomology and Economic Zoology, Cook College, Rutgers University, P.O. Box 231, New Brunswick, 08903, New Jersey, United States
Development of malaria parasites in mosquitoes fed with ookinetes suspended in defined media
Information concerning the specific nutritional requirements of malarial parasites developing in the mosquito host has been difficult to obtain, owing primarily to the complex nature of the blood meal that accompanies the parasites and the lack of success in culturing the complete invertebrate cycle of Plasmodium in vitro. The present report describes a blood-free system for infecting mosquitoes with ookinetes of Plasmodium berghei and for allowing the latter to develop into infective sporozoites. Ookinetes cultured in vitro were separated from blood proteins, suspended in defined medium, and fed to Anopheles stephensi mosquitoes through a membrane. The mosquitoes were then maintained on the same defined medium plus 5% sucrose. Infectivity of the parasites was demonstrated 17-19 days later by intracardial inoculation of the macerated mosquitoes into hamsters. This system makes it possible to evaluate nutritional factors that affect parasite development in the mosquito host under controlled conditions. © 1990 Birkhäuser Verlag Basel.
Scientific Publication
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