נגישות
menu      
חיפוש מתקדם
תחביר
חפש...
הספר "אוצר וולקני"
אודות
תנאי שימוש
ניהול
קהילה:
אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Enhanced oxidative-stress defense in transgenic potato expressing tomato Cu,Zn superoxide dismutases
Year:
1993
Source of publication :
Theoretical and Applied Genetics
Authors :
גלילי, שמואל
;
.
Volume :
85
Co-Authors:
Perl, A., Department of Plant Genetics, Weizmann Institute of Science, Rehovot, 76100, Israel
Perl-Treves, R., Department of Plant Genetics, Weizmann Institute of Science, Rehovot, 76100, Israel
Galili, S., Department of Plant Genetics, Weizmann Institute of Science, Rehovot, 76100, Israel
Aviv, D., Department of Plant Genetics, Weizmann Institute of Science, Rehovot, 76100, Israel
Shalgi, E., Department of Biochemistry, The Weizmann Institute of Science, Rehovot, 76100, Israel
Malkin, S., Department of Biochemistry, The Weizmann Institute of Science, Rehovot, 76100, Israel
Galun, E., Department of Plant Genetics, Weizmann Institute of Science, Rehovot, 76100, Israel
Facilitators :
From page:
568
To page:
576
(
Total pages:
9
)
Abstract:
The two cDNAs coding for the cytosolic (cyt) and the chloroplast-located (chl) Cu,Zn superoxide dismutases (SODs) of tomato (Perl-Treves et al. 1988) were cloned into respective binary vectors and mobilized into Agrobacterium strains. Potato tuber discs were infected with either of the two agrobacterial strains and cultured on selective medium containing kanaymcin. The integration of either of the cyt or the chl SOD transgenes was verified by Southern-blot hybridization. The enzymatic activity of the additional tomato chl Cu,Zn SOD could be distinguished from endogenous SOD activity since the latter isozyme migrated faster on SOD-activity gels. Several transgenic potato lines harboring either the cyt or the chl SOD genes of tomato showed elevated tolerance to the superoxide-generating herbicide paraquat (methyl viologen). After exposure of shoots to paraquat, tolerance was recorded either by scoring symptoms visually or by measurements of photosynthesis using the photoacoustic method. Root cultures from transgenic lines that harbored the additional cyt Cu,Zn SOD gene of tomato were tolerant to methyl viologen up to 10-5 M; a lower tolerance was recorded in roots of transgenic lines that expressed the additional chl Cu,Zn SOD of tomato. © 1993 Springer-Verlag.
Note:
Related Files :
Lycopersicon esculentum
Oxygen toxicity
Paraquat
Photoacoustic spectroscopy
Solanum tuberosum
transformation
עוד תגיות
תוכן קשור
More details
DOI :
10.1007/BF00220915
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
25385
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:14
You may also be interested in
Scientific Publication
Enhanced oxidative-stress defense in transgenic potato expressing tomato Cu,Zn superoxide dismutases
85
Perl, A., Department of Plant Genetics, Weizmann Institute of Science, Rehovot, 76100, Israel
Perl-Treves, R., Department of Plant Genetics, Weizmann Institute of Science, Rehovot, 76100, Israel
Galili, S., Department of Plant Genetics, Weizmann Institute of Science, Rehovot, 76100, Israel
Aviv, D., Department of Plant Genetics, Weizmann Institute of Science, Rehovot, 76100, Israel
Shalgi, E., Department of Biochemistry, The Weizmann Institute of Science, Rehovot, 76100, Israel
Malkin, S., Department of Biochemistry, The Weizmann Institute of Science, Rehovot, 76100, Israel
Galun, E., Department of Plant Genetics, Weizmann Institute of Science, Rehovot, 76100, Israel
Enhanced oxidative-stress defense in transgenic potato expressing tomato Cu,Zn superoxide dismutases
The two cDNAs coding for the cytosolic (cyt) and the chloroplast-located (chl) Cu,Zn superoxide dismutases (SODs) of tomato (Perl-Treves et al. 1988) were cloned into respective binary vectors and mobilized into Agrobacterium strains. Potato tuber discs were infected with either of the two agrobacterial strains and cultured on selective medium containing kanaymcin. The integration of either of the cyt or the chl SOD transgenes was verified by Southern-blot hybridization. The enzymatic activity of the additional tomato chl Cu,Zn SOD could be distinguished from endogenous SOD activity since the latter isozyme migrated faster on SOD-activity gels. Several transgenic potato lines harboring either the cyt or the chl SOD genes of tomato showed elevated tolerance to the superoxide-generating herbicide paraquat (methyl viologen). After exposure of shoots to paraquat, tolerance was recorded either by scoring symptoms visually or by measurements of photosynthesis using the photoacoustic method. Root cultures from transgenic lines that harbored the additional cyt Cu,Zn SOD gene of tomato were tolerant to methyl viologen up to 10-5 M; a lower tolerance was recorded in roots of transgenic lines that expressed the additional chl Cu,Zn SOD of tomato. © 1993 Springer-Verlag.
Scientific Publication
You may also be interested in