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פותח על ידי קלירמאש פתרונות בע"מ -
A role of AREB in the regulation of PACC-dependent acid-expressed-genes and pathogenicity of Colletotrichum gloeosporioides
Year:
2015
Authors :
אלקן, נעם
;
.
מנט, דנה
;
.
פרוסקי, דב
;
.
Volume :
28
Co-Authors:
Ment, D., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Alkan, N., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Luria, N., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Bi, F.-C., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Reuveni, E., Department of Plant Sciences, Weizmann Institute of Science, Rehovot, Israel
Fluhr, R., Department of Plant Sciences, Weizmann Institute of Science, Rehovot, Israel
Prusky, D., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Facilitators :
From page:
154
To page:
166
(
Total pages:
13
)
Abstract:
Gene expression regulation by pH in filamentous fungi and yeasts is controlled by the PACC/RIM101 transcription factor. In Colletotrichum gloeosporioides, PACC is known to act as positive regulator of alkaline-expressed genes, and this regulation was shown to contribute to fungal pathogenicity. PACC is also a negative regulator of acid-expressed genes, however; the mechanism of downregulation of acidexpressed genes by PACC and their contribution to C. gloeosporioides pathogenicity is not well understood. RNA sequencing data analysis was employed to demonstrate that PACC transcription factor binding sites (TFBS) are significantly overrepresented in the promoter of PACC-upregulated, alkaline-expressed genes. In contrast, they are not overrepresented in the PACC-downregulated, acid-expressed genes. Instead, acid-expressed genes showed overrepresentation of AREB GATA TFBS in C. gloeosporioides and in homologs of five other ascomycetes genomes. The areB promoter contains PACC TFBS; its transcript was upregulated at pH 7 and repressed in ΔpacC. Furthermore, acid-expressed genes were found to be constitutively upregulated in ΔareB during alkalizing conditions. The areB mutants showed significantly reduced ammonia secretion and pathogenicity on tomato fruit. Present results indicate that PACC activates areB expression, thereby conditionally repressing acid-expressed genes and contributing critically to C. gloeosporioides pathogenicity. © 2015 The American Phytopathological Society.
Note:
Related Files :
Colletotrichum
fungi
Genetics
metabolism
Microbiology
molecular genetics
Plant Disease
Plant Diseases
עוד תגיות
תוכן קשור
More details
DOI :
10.1094/MPMI-09-14-0252-R
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
25536
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:15
You may also be interested in
Scientific Publication
A role of AREB in the regulation of PACC-dependent acid-expressed-genes and pathogenicity of Colletotrichum gloeosporioides
28
Ment, D., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Alkan, N., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Luria, N., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Bi, F.-C., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Reuveni, E., Department of Plant Sciences, Weizmann Institute of Science, Rehovot, Israel
Fluhr, R., Department of Plant Sciences, Weizmann Institute of Science, Rehovot, Israel
Prusky, D., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
A role of AREB in the regulation of PACC-dependent acid-expressed-genes and pathogenicity of Colletotrichum gloeosporioides
Gene expression regulation by pH in filamentous fungi and yeasts is controlled by the PACC/RIM101 transcription factor. In Colletotrichum gloeosporioides, PACC is known to act as positive regulator of alkaline-expressed genes, and this regulation was shown to contribute to fungal pathogenicity. PACC is also a negative regulator of acid-expressed genes, however; the mechanism of downregulation of acidexpressed genes by PACC and their contribution to C. gloeosporioides pathogenicity is not well understood. RNA sequencing data analysis was employed to demonstrate that PACC transcription factor binding sites (TFBS) are significantly overrepresented in the promoter of PACC-upregulated, alkaline-expressed genes. In contrast, they are not overrepresented in the PACC-downregulated, acid-expressed genes. Instead, acid-expressed genes showed overrepresentation of AREB GATA TFBS in C. gloeosporioides and in homologs of five other ascomycetes genomes. The areB promoter contains PACC TFBS; its transcript was upregulated at pH 7 and repressed in ΔpacC. Furthermore, acid-expressed genes were found to be constitutively upregulated in ΔareB during alkalizing conditions. The areB mutants showed significantly reduced ammonia secretion and pathogenicity on tomato fruit. Present results indicate that PACC activates areB expression, thereby conditionally repressing acid-expressed genes and contributing critically to C. gloeosporioides pathogenicity. © 2015 The American Phytopathological Society.
Scientific Publication
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