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פותח על ידי קלירמאש פתרונות בע"מ -
The third wave of myotome colonization by mitotically competent progenitors: Regulating the balance between differentiation and proliferation during muscle development
Year:
2001
Source of publication :
Development
Authors :
צינמון, יובל
;
.
Volume :
128
Co-Authors:
Kahane, N., Department of Anatomy/Cell Biology, Hebrew Univ.-Hadassah Medical School, PO Box 12272, Jerusalem 91120, Israel
Cinnamon, Y., Department of Anatomy/Cell Biology, Hebrew Univ.-Hadassah Medical School, PO Box 12272, Jerusalem 91120, Israel
Bachelet, I., Department of Anatomy/Cell Biology, Hebrew Univ.-Hadassah Medical School, PO Box 12272, Jerusalem 91120, Israel
Kalcheim, C., Department of Anatomy/Cell Biology, Hebrew Univ.-Hadassah Medical School, PO Box 12272, Jerusalem 91120, Israel
Facilitators :
From page:
2187
To page:
2198
(
Total pages:
12
)
Abstract:
The myotome is formed by a first wave of pioneer cells originating from the entire dorsomedial region of epithelial somites and a second wave that derives from all four lips of the dermomyotome but generates myofibers from only the rostral and caudal edges. Because the precedent progenitors exit the cell cycle upon myotome colonization, subsequent waves must account for consecutive growth. In this study, double labeling with CM-DiI and BrdU revealed the appearance of a third wave of progenitors that enter the myotome as mitotically active cells from both rostral and caudal dermomyotome edges. These cells express the fibroblast growth factor (FGF) receptor FREK and treatment with FGF4 promotes their proliferation and redistribution towards the center of the myotome. Yet, they are negative for MyoD, Myf5 and FGF4, which are, however, expressed in myofibers. The proliferating progenitors first appear around the 30-somite stage in cervical-level myotomes and their number continuously increases, making up 85% of total muscle nuclei by embryonic day (E)4. By this stage, generation of second-wave myofibers, which also enter from the extreme lips is still under way. Formation of the latter fibers peaks at 30 somites and progressively decreases with age until E4. Thus, cells in these dermomyotome lips generate simultaneously distinct types of muscle progenitors in changing proportions as a function of age. Consistent with a heterogeneity in the cellular composition of the extreme lips, MyoD is normally expressed in only a subset of these epithelial cells. Treatment with Sonic hedgehog drives most of them to become MyoD positive and then to become myofibers, with a concurrent reduction in the proportion of proliferating muscle precursors.
Note:
Related Files :
Animals
Cell Proliferation
gene expression
Proliferation
Receptor Protein-Tyrosine Kinases
stem cells
עוד תגיות
תוכן קשור
More details
DOI :
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
25570
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:16
Scientific Publication
The third wave of myotome colonization by mitotically competent progenitors: Regulating the balance between differentiation and proliferation during muscle development
128
Kahane, N., Department of Anatomy/Cell Biology, Hebrew Univ.-Hadassah Medical School, PO Box 12272, Jerusalem 91120, Israel
Cinnamon, Y., Department of Anatomy/Cell Biology, Hebrew Univ.-Hadassah Medical School, PO Box 12272, Jerusalem 91120, Israel
Bachelet, I., Department of Anatomy/Cell Biology, Hebrew Univ.-Hadassah Medical School, PO Box 12272, Jerusalem 91120, Israel
Kalcheim, C., Department of Anatomy/Cell Biology, Hebrew Univ.-Hadassah Medical School, PO Box 12272, Jerusalem 91120, Israel
The third wave of myotome colonization by mitotically competent progenitors: Regulating the balance between differentiation and proliferation during muscle development
The myotome is formed by a first wave of pioneer cells originating from the entire dorsomedial region of epithelial somites and a second wave that derives from all four lips of the dermomyotome but generates myofibers from only the rostral and caudal edges. Because the precedent progenitors exit the cell cycle upon myotome colonization, subsequent waves must account for consecutive growth. In this study, double labeling with CM-DiI and BrdU revealed the appearance of a third wave of progenitors that enter the myotome as mitotically active cells from both rostral and caudal dermomyotome edges. These cells express the fibroblast growth factor (FGF) receptor FREK and treatment with FGF4 promotes their proliferation and redistribution towards the center of the myotome. Yet, they are negative for MyoD, Myf5 and FGF4, which are, however, expressed in myofibers. The proliferating progenitors first appear around the 30-somite stage in cervical-level myotomes and their number continuously increases, making up 85% of total muscle nuclei by embryonic day (E)4. By this stage, generation of second-wave myofibers, which also enter from the extreme lips is still under way. Formation of the latter fibers peaks at 30 somites and progressively decreases with age until E4. Thus, cells in these dermomyotome lips generate simultaneously distinct types of muscle progenitors in changing proportions as a function of age. Consistent with a heterogeneity in the cellular composition of the extreme lips, MyoD is normally expressed in only a subset of these epithelial cells. Treatment with Sonic hedgehog drives most of them to become MyoD positive and then to become myofibers, with a concurrent reduction in the proportion of proliferating muscle precursors.
Scientific Publication
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