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פותח על ידי קלירמאש פתרונות בע"מ -
Gold-conjugated reagents for the labelling of carbohydrate-recognition domains and glycoconjugates on nematode surfaces
Year:
1996
Source of publication :
Journal of Nematology
Authors :
שפיגל, יצחק
;
.
שרון, עדנה
;
.
Volume :
28
Co-Authors:
Sharon, E., Department of Nematology, ARO, Volcani Center, Bet Dagan 50-250, Israel
Spiegel, Y., Department of Nematology, ARO, Volcani Center, Bet Dagan 50-250, Israel
Facilitators :
From page:
124
To page:
127
(
Total pages:
4
)
Abstract:
Various fluorescent conjugated lectins have been used for the detection of glycoconjugates on nematode surfaces under light microscopy. Several problems have been experienced with these reagents including penetration of the cuticle by fluorescent lectins, non-glycoconjugate specificity, strong nematode autofluorescence at the emission wavelength of the fluorescent dye, and prevention of persistent visualization due to rapid quenching of the fluorescent components. Gold-conjugated reagents combined with silver enhancement alleviated these difficulties when working with three phytonematode species (Heterodera avenae, H. latipons, and Meloidogyne javanica) and two entomopathogenic species (Steinernema carpocapsae and S. glaseri) under light-microscopy visualization of binding by fluorescent lectins and neoglycoproteins. Moreover, gold-conjugated reagents resulted in stable bindings that enabled long-term observations.
Note:
Related Files :
concanavalin A
Fluorescent
Glycoconjugate
Heterodera avenae
Heterodera latipons
nematode
Neoglycoprotein
Steinernema carpocapsae
עוד תגיות
תוכן קשור
More details
DOI :
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
25576
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:16
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Scientific Publication
Gold-conjugated reagents for the labelling of carbohydrate-recognition domains and glycoconjugates on nematode surfaces
28
Sharon, E., Department of Nematology, ARO, Volcani Center, Bet Dagan 50-250, Israel
Spiegel, Y., Department of Nematology, ARO, Volcani Center, Bet Dagan 50-250, Israel
Gold-conjugated reagents for the labelling of carbohydrate-recognition domains and glycoconjugates on nematode surfaces
Various fluorescent conjugated lectins have been used for the detection of glycoconjugates on nematode surfaces under light microscopy. Several problems have been experienced with these reagents including penetration of the cuticle by fluorescent lectins, non-glycoconjugate specificity, strong nematode autofluorescence at the emission wavelength of the fluorescent dye, and prevention of persistent visualization due to rapid quenching of the fluorescent components. Gold-conjugated reagents combined with silver enhancement alleviated these difficulties when working with three phytonematode species (Heterodera avenae, H. latipons, and Meloidogyne javanica) and two entomopathogenic species (Steinernema carpocapsae and S. glaseri) under light-microscopy visualization of binding by fluorescent lectins and neoglycoproteins. Moreover, gold-conjugated reagents resulted in stable bindings that enabled long-term observations.
Scientific Publication
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