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פותח על ידי קלירמאש פתרונות בע"מ -
Lipoxygenase and other enzymes of arachidonic acid metabolism in the brain of chicks affected by nutritional encephalomalacia
Year:
1993
Authors :
גרינברג-לוי, סימה
;
.
Volume :
25
Co-Authors:
Greenberg-Levy, S.H., Department of Life Sciences, Bar-Ilan University, Ramat-Gan 52900, Israel [Tel.: (03) 5318212
Fax: (03) 771088]
Budowski, P., Faculty of Agriculture, The Hebrew University of Jerusalem, Rehovot, 76100, Israel
Grossman, S., Department of Life Sciences, Bar-Ilan University, Ramat-Gan 52900, Israel [Tel.: (03) 5318212
Facilitators :
From page:
403
To page:
409
(
Total pages:
7
)
Abstract:
1. 1. Prostaglandin endoperoxide synthetase (PES) and lipoxygenase (Lox) activities were compared in the cerebella and cerebra of vitamin E-sufficient young chicks and in chicks in which nutritional encephalomalacia (NE) was induced by a diet deficient in vitamin E. 2. 2. Eicosanoid production patterns were qualitatively similar in the brains of both groups of chicks, but prostaglandin production was 50-60% less in cerebella of ataxic chicks, compared to control cerebella, while the opposite trend was observed in the cerebellar Lox pathway, as measured by radioimmunoassay of 15-HETE. 3. 3. Cerebellar phospholipase A, activity was twice that of the cerebrum but was not affected by NE. 4. 4. Purification of Lox activity from the cerebellar homogenates produced a lower yield and enrichment when the starting material was taken from ataxic chicks, compared to the controls. 5. 5. In addition there were qualitative differences in the purified fractions from both groups, as seen by pH optima and kinetics. 6. 6. The results are consistent with the view that the cerebellum has less antioxidant protection than the cerebrum and that its higher phospholipase A2 activity and greater propensity to oxygenate arachidonic acid via the Lox pathway at the expense of the PES pathway may render this region of the brain particularly vulnerable to oxidative damage in NE. © 1993.
Note:
Related Files :
Animal
animal experiment
animal tissue
Chickens
Male
Prostaglandins
עוד תגיות
תוכן קשור
More details
DOI :
10.1016/0020-711X(93)90631-N
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
25578
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:16
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Scientific Publication
Lipoxygenase and other enzymes of arachidonic acid metabolism in the brain of chicks affected by nutritional encephalomalacia
25
Greenberg-Levy, S.H., Department of Life Sciences, Bar-Ilan University, Ramat-Gan 52900, Israel [Tel.: (03) 5318212
Fax: (03) 771088]
Budowski, P., Faculty of Agriculture, The Hebrew University of Jerusalem, Rehovot, 76100, Israel
Grossman, S., Department of Life Sciences, Bar-Ilan University, Ramat-Gan 52900, Israel [Tel.: (03) 5318212
Lipoxygenase and other enzymes of arachidonic acid metabolism in the brain of chicks affected by nutritional encephalomalacia
1. 1. Prostaglandin endoperoxide synthetase (PES) and lipoxygenase (Lox) activities were compared in the cerebella and cerebra of vitamin E-sufficient young chicks and in chicks in which nutritional encephalomalacia (NE) was induced by a diet deficient in vitamin E. 2. 2. Eicosanoid production patterns were qualitatively similar in the brains of both groups of chicks, but prostaglandin production was 50-60% less in cerebella of ataxic chicks, compared to control cerebella, while the opposite trend was observed in the cerebellar Lox pathway, as measured by radioimmunoassay of 15-HETE. 3. 3. Cerebellar phospholipase A, activity was twice that of the cerebrum but was not affected by NE. 4. 4. Purification of Lox activity from the cerebellar homogenates produced a lower yield and enrichment when the starting material was taken from ataxic chicks, compared to the controls. 5. 5. In addition there were qualitative differences in the purified fractions from both groups, as seen by pH optima and kinetics. 6. 6. The results are consistent with the view that the cerebellum has less antioxidant protection than the cerebrum and that its higher phospholipase A2 activity and greater propensity to oxygenate arachidonic acid via the Lox pathway at the expense of the PES pathway may render this region of the brain particularly vulnerable to oxidative damage in NE. © 1993.
Scientific Publication
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