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פותח על ידי קלירמאש פתרונות בע"מ -
Hsp90 and angiogenesis in bone disorders-lessons from the avian growth plate
Year:
2011
Authors :
גנין, אולגה
;
.
הרצוג, איילת
;
.
חסדאי, אהרון
;
.
פינס, מרק
;
.
שינדר, דמיטרי
;
.
Volume :
301
Co-Authors:
Herzog, A., Institute of Animal Sciences, The Volcani Center, Bet Dagan, Israel
Genin, O., Institute of Animal Sciences, The Volcani Center, Bet Dagan, Israel
Hasdai, A., Institute of Animal Sciences, The Volcani Center, Bet Dagan, Israel
Shinder, D., Institute of Animal Sciences, The Volcani Center, Bet Dagan, Israel
Pines, M., Institute of Animal Sciences, The Volcani Center, Bet Dagan, Israel
Facilitators :
From page:
To page:
(
Total pages:
1
)
Abstract:
Thiram-induced tibial dyschondroplasia (TD) and vitamin-D deficiency rickets are avian bone disorders of different etiologies characterized by abnormal chondrocyte differentiation, enlarged and unvascularized growth plates, and lameness. Heat-shock protein 90 (Hsp90) is a proangiogenic factor in mammalian tissues and in tumors; therefore, Hsp90 inhibitors were developed as antiangiogenic factors. In this study, we evaluated the association between Hsp90, hypoxia, and angiogenesis in the chick growth plate. Administration of the Hsp90 inhibitor to TD- and rickets-afflicted chicks at the time of induction resulted in reduction in growth-plate size and, contrary to its antiangiogenic effect in tumors, a major invasion of blood vessels occurred in the growth plates. This was the result of upregulation of the VEGF receptor Flk-1, the major rate-limiting factor of vascularization in TD and rickets. In addition, the abnormal chondrocyte differentiation, as characterized by collagen type II expression and alkaline phosphatase activity, and the changes in hypoxia-inducible factor-1 α (HIF-1α) in both disorders were restored. All these changes resulted in prevention of lameness. Inhibition of Hsp90 activity reduced growth-plate size, increased vascularization, and mitigated lameness also in TD chickswith established lesions. In summary, this is the first reported demonstration of involvement of Hsp90 in chondrocyte differentiation and growth-plate vascularization. In contrast to the antiangiogenic effect of Hsp90 inhibitors observed in mammals, inhibition of Hsp90 activity in the unvascularized TD- and rickets-afflicted chicks resulted in activation of the angiogenic switch and reinstated normal growthplate morphology. © 2011 the American Physiological Society.
Note:
Related Files :
animal experiment
Animals
animal tissue
gene expression
hypoxia
Male
vasculotropin receptor 2
vitamin blood level
עוד תגיות
תוכן קשור
More details
DOI :
10.1152/ajpregu.00134.2011
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
25587
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:16
You may also be interested in
Scientific Publication
Hsp90 and angiogenesis in bone disorders-lessons from the avian growth plate
301
Herzog, A., Institute of Animal Sciences, The Volcani Center, Bet Dagan, Israel
Genin, O., Institute of Animal Sciences, The Volcani Center, Bet Dagan, Israel
Hasdai, A., Institute of Animal Sciences, The Volcani Center, Bet Dagan, Israel
Shinder, D., Institute of Animal Sciences, The Volcani Center, Bet Dagan, Israel
Pines, M., Institute of Animal Sciences, The Volcani Center, Bet Dagan, Israel
Hsp90 and angiogenesis in bone disorders-lessons from the avian growth plate
Thiram-induced tibial dyschondroplasia (TD) and vitamin-D deficiency rickets are avian bone disorders of different etiologies characterized by abnormal chondrocyte differentiation, enlarged and unvascularized growth plates, and lameness. Heat-shock protein 90 (Hsp90) is a proangiogenic factor in mammalian tissues and in tumors; therefore, Hsp90 inhibitors were developed as antiangiogenic factors. In this study, we evaluated the association between Hsp90, hypoxia, and angiogenesis in the chick growth plate. Administration of the Hsp90 inhibitor to TD- and rickets-afflicted chicks at the time of induction resulted in reduction in growth-plate size and, contrary to its antiangiogenic effect in tumors, a major invasion of blood vessels occurred in the growth plates. This was the result of upregulation of the VEGF receptor Flk-1, the major rate-limiting factor of vascularization in TD and rickets. In addition, the abnormal chondrocyte differentiation, as characterized by collagen type II expression and alkaline phosphatase activity, and the changes in hypoxia-inducible factor-1 α (HIF-1α) in both disorders were restored. All these changes resulted in prevention of lameness. Inhibition of Hsp90 activity reduced growth-plate size, increased vascularization, and mitigated lameness also in TD chickswith established lesions. In summary, this is the first reported demonstration of involvement of Hsp90 in chondrocyte differentiation and growth-plate vascularization. In contrast to the antiangiogenic effect of Hsp90 inhibitors observed in mammals, inhibition of Hsp90 activity in the unvascularized TD- and rickets-afflicted chicks resulted in activation of the angiogenic switch and reinstated normal growthplate morphology. © 2011 the American Physiological Society.
Scientific Publication
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