חיפוש מתקדם
תחביר
חפש...
הספר "אוצר וולקני"
אודות
תנאי שימוש
ניהול
קהילה:
אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Characterization of insulin-like growth factor-binding proteins in the uterus and conceptus during early conceptus elongation in cattle
Year:
1998
Source of publication :
Biology of Reproduction
Authors :
קלר, מנחם
;
.
Volume :
59
Co-Authors:
Keller, M.L., Anim. Repro. and Biotech. Laboratory, Colorado State University, Fort Collins, CO 80523-1683, United States
Roberts, A.J., USDA-ARS, Roman L. Hruska U.S. Meat Anim. R., Clay Center, NE 68933-0166, United States, USDA-ARS, U.S. Meat Animal Research Center, P.O. Box 166, Clay Center, NE 68933-0166, United States
Seidel Jr., G.E., Anim. Repro. and Biotech. Laboratory, Colorado State University, Fort Collins, CO 80523-1683, United States
Facilitators :
From page:
632
To page:
642
(
Total pages:
11
)
Abstract:
As a first step in determining the role that insulin-like growth factor (IGF)-binding proteins (BPs) may have in regulating initial stages of conceptus elongation in cattle, the type and relative abundance of IGFBPs in serum, uterine tissues, and uterine fluid from pregnant and noninseminated cows on Days 13 and 15 postestrus and in Day 15 conceptuses was evaluated. Uterine and serum samples contained IGFBPs 2, 3, 4, and 5 as determined by immunoprecipitation followed by Western ligand blots of precipitates. Compared with those in uterine and serum samples, IGFBPs in conceptuses and conceptus-conditioned culture media were only faintly detectable. The percentage of the total IGF-I binding activity attributed to IGFBP-3 was greater (p < 0.05) in myometrium, serum, and uterine fluid (> 50%) than in inter. (40%) and intracaruncular (37%) endometrium. Percentage of total binding attributed to IGFBP-2 was greater (p < 0.05) in endometrium and serum (~30%) than in myometrium (16%) and uterine fluid (9%). Binding activity of certain IGFBPs varied due to day of the estrous cycle or due to pregnancy status. Concentrations of IGF-I in serum were greater (p < 0.05) in nonpregnant (52 ± 2 ng/ml) than in pregnant (40 ± 4 ng/ml) cows. Concentration of IGF-I in uterine fluid did not differ due to pregnancy status or stage of cycle (4.4 pg IGF-I/μg uterine protein). Northern blots revealed mRNAs for IGFBPs 1, 2, 3, 4, and 5 in uterine tissues but not in conceptuses. In situ hybridization indicated that IGFBP-1 mRNA was primarily localized in luminal epithelium of endometrium; IGFBP-2 mRNA was in luminal epithelium and dense stromal cells adjacent to endometrial epithelium; and IGFBP-3 mRNA was in vascular endothelial cells and was more prevalent in myometrium than in endometrium. Tissue specificity and changes in abundance of IGFBPs in the uterus during early conceptus elongation indicate the potential importance of IGFBP regulation of uterine IGFs during this time period.
Note:
Related Files :
Animals
animal tissue
cattle
endometrium
Female
immunoprecipitation
Insulin-Like Growth Factor I
myometrium
pregnancy
עוד תגיות
תוכן קשור
More details
DOI :
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
25743
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:17
You may also be interested in
Scientific Publication
Characterization of insulin-like growth factor-binding proteins in the uterus and conceptus during early conceptus elongation in cattle
59
Keller, M.L., Anim. Repro. and Biotech. Laboratory, Colorado State University, Fort Collins, CO 80523-1683, United States
Roberts, A.J., USDA-ARS, Roman L. Hruska U.S. Meat Anim. R., Clay Center, NE 68933-0166, United States, USDA-ARS, U.S. Meat Animal Research Center, P.O. Box 166, Clay Center, NE 68933-0166, United States
Seidel Jr., G.E., Anim. Repro. and Biotech. Laboratory, Colorado State University, Fort Collins, CO 80523-1683, United States
Characterization of insulin-like growth factor-binding proteins in the uterus and conceptus during early conceptus elongation in cattle
As a first step in determining the role that insulin-like growth factor (IGF)-binding proteins (BPs) may have in regulating initial stages of conceptus elongation in cattle, the type and relative abundance of IGFBPs in serum, uterine tissues, and uterine fluid from pregnant and noninseminated cows on Days 13 and 15 postestrus and in Day 15 conceptuses was evaluated. Uterine and serum samples contained IGFBPs 2, 3, 4, and 5 as determined by immunoprecipitation followed by Western ligand blots of precipitates. Compared with those in uterine and serum samples, IGFBPs in conceptuses and conceptus-conditioned culture media were only faintly detectable. The percentage of the total IGF-I binding activity attributed to IGFBP-3 was greater (p < 0.05) in myometrium, serum, and uterine fluid (> 50%) than in inter. (40%) and intracaruncular (37%) endometrium. Percentage of total binding attributed to IGFBP-2 was greater (p < 0.05) in endometrium and serum (~30%) than in myometrium (16%) and uterine fluid (9%). Binding activity of certain IGFBPs varied due to day of the estrous cycle or due to pregnancy status. Concentrations of IGF-I in serum were greater (p < 0.05) in nonpregnant (52 ± 2 ng/ml) than in pregnant (40 ± 4 ng/ml) cows. Concentration of IGF-I in uterine fluid did not differ due to pregnancy status or stage of cycle (4.4 pg IGF-I/μg uterine protein). Northern blots revealed mRNAs for IGFBPs 1, 2, 3, 4, and 5 in uterine tissues but not in conceptuses. In situ hybridization indicated that IGFBP-1 mRNA was primarily localized in luminal epithelium of endometrium; IGFBP-2 mRNA was in luminal epithelium and dense stromal cells adjacent to endometrial epithelium; and IGFBP-3 mRNA was in vascular endothelial cells and was more prevalent in myometrium than in endometrium. Tissue specificity and changes in abundance of IGFBPs in the uterus during early conceptus elongation indicate the potential importance of IGFBP regulation of uterine IGFs during this time period.
Scientific Publication
נגישות
menu      
You may also be interested in