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אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Immunochemical approaches for purification and detection of TNT traces by antibodies entrapped in a sol-gel matrix
Year:
2001
Source of publication :
Analytical Chemistry
Authors :
אלטשטיין, מרים
;
.
ברונשטיין, אליסה
;
.
Volume :
73
Co-Authors:
Altstein, M., Department of Cell Biology and Neurosciences, University of California Riverside, Boyce Hall 5419, Riverside, CA 92521-0146, United States, Institute of Plant Protection, Volcani Center, Bet Dagan 50250, Israel
Bronshtein, A., Institute of Plant Protection, Volcani Center, Bet Dagan 50250, Israel
Glattstein, B., Division of Identification and Forensic Science, Israel National Police Headquarters, Jerusalem 91906, Israel
Zeichner, A., Division of Identification and Forensic Science, Israel National Police Headquarters, Jerusalem 91906, Israel
Tamiri, T., Division of Identification and Forensic Science, Israel National Police Headquarters, Jerusalem 91906, Israel
Almog, J., Division of Identification and Forensic Science, Israel National Police Headquarters, Jerusalem 91906, Israel
Facilitators :
From page:
2461
To page:
2467
(
Total pages:
7
)
Abstract:
A highly sensitive immunochemical method for immunoaffinity purification (IAP) and detection of trace amounts of TNT was developed on the basis of antibodies (Abs) in a ceramic matrix (sol-gel). The study resulted in: (i) a highly sensitive and reproducible TNT ELISA (I50 and I20 values of 0.4 ± 0.09 ppb and 0.12 ± 0.03 ppb, respectively, n = 12), which is highly specific to TNT; and (ii) successful entrapment of the Abs that bound free analyte from solution. Binding was found to be highly reproducible, dose dependent, and only slightly (1.2-1.8-fold) lower than that in solution. The entrapped Abs did not leach from the matrix and were tolerant of absolute ethanol, acetone, and acetonitrile. Bound analytes could be easily eluted from the sol-gel matrix at high recoveries. The sol-gel-based IAP method described above introduces a simple one-step procedure that has a high potential to serve as a suitable and convenient immunochromatographic device for cleanup and concentration of TNT from "real field" samples in a manner that complies with both chemical and immunochemical residue analysis methods.
Note:
Related Files :
acetone
acetonitrile
alcohol
chemical binding
gel
sensitivity and specificity
עוד תגיות
תוכן קשור
More details
DOI :
10.1021/ac001376y
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
25760
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:17
You may also be interested in
Scientific Publication
Immunochemical approaches for purification and detection of TNT traces by antibodies entrapped in a sol-gel matrix
73
Altstein, M., Department of Cell Biology and Neurosciences, University of California Riverside, Boyce Hall 5419, Riverside, CA 92521-0146, United States, Institute of Plant Protection, Volcani Center, Bet Dagan 50250, Israel
Bronshtein, A., Institute of Plant Protection, Volcani Center, Bet Dagan 50250, Israel
Glattstein, B., Division of Identification and Forensic Science, Israel National Police Headquarters, Jerusalem 91906, Israel
Zeichner, A., Division of Identification and Forensic Science, Israel National Police Headquarters, Jerusalem 91906, Israel
Tamiri, T., Division of Identification and Forensic Science, Israel National Police Headquarters, Jerusalem 91906, Israel
Almog, J., Division of Identification and Forensic Science, Israel National Police Headquarters, Jerusalem 91906, Israel
Immunochemical approaches for purification and detection of TNT traces by antibodies entrapped in a sol-gel matrix
A highly sensitive immunochemical method for immunoaffinity purification (IAP) and detection of trace amounts of TNT was developed on the basis of antibodies (Abs) in a ceramic matrix (sol-gel). The study resulted in: (i) a highly sensitive and reproducible TNT ELISA (I50 and I20 values of 0.4 ± 0.09 ppb and 0.12 ± 0.03 ppb, respectively, n = 12), which is highly specific to TNT; and (ii) successful entrapment of the Abs that bound free analyte from solution. Binding was found to be highly reproducible, dose dependent, and only slightly (1.2-1.8-fold) lower than that in solution. The entrapped Abs did not leach from the matrix and were tolerant of absolute ethanol, acetone, and acetonitrile. Bound analytes could be easily eluted from the sol-gel matrix at high recoveries. The sol-gel-based IAP method described above introduces a simple one-step procedure that has a high potential to serve as a suitable and convenient immunochromatographic device for cleanup and concentration of TNT from "real field" samples in a manner that complies with both chemical and immunochemical residue analysis methods.
Scientific Publication
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