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פותח על ידי קלירמאש פתרונות בע"מ -
TraceHaplotyper: Using direct sequencing to determine the phase of an indel followed by biallelic SNPs
Year:
2007
Source of publication :
BioTechniques
Authors :
סרוסי, אייל
;
.
Volume :
43
Co-Authors:
Seroussi, Y., Technion-Israel Institute of Technology, Haifa, Israel
Seroussi, E., Volcani Center, Bet-Dagan, Israel, Agricultural Research Organization, Volcani Center, Institute of Animal Science, P.O.B 6, Bet Dagan 5020, Israel
Facilitators :
From page:
452
To page:
456
(
Total pages:
5
)
Abstract:
Sequencing of a diploid PCR product that is heterozygous for an indel mutation and a downstream single nucleotide polymorphism (SNP) allows determination of haplotype phase. Manual determination of phase from trace files of this type is tedious; therefore, we introduce the Perl script TraceHaplotyper, which works from the expected sequence to analyze a sequencing trace file for the sites that are informative for the genotyping and outputs the two underlying haplotypes.
Note:
Related Files :
Base Sequence
gene sequence
genetic analysis
genetic engineering
genetic markers
INDEL Mutation
עוד תגיות
תוכן קשור
More details
DOI :
10.2144/000112552
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
25778
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:17
You may also be interested in
Scientific Publication
TraceHaplotyper: Using direct sequencing to determine the phase of an indel followed by biallelic SNPs
43
Seroussi, Y., Technion-Israel Institute of Technology, Haifa, Israel
Seroussi, E., Volcani Center, Bet-Dagan, Israel, Agricultural Research Organization, Volcani Center, Institute of Animal Science, P.O.B 6, Bet Dagan 5020, Israel
TraceHaplotyper: Using direct sequencing to determine the phase of an indel followed by biallelic SNPs
Sequencing of a diploid PCR product that is heterozygous for an indel mutation and a downstream single nucleotide polymorphism (SNP) allows determination of haplotype phase. Manual determination of phase from trace files of this type is tedious; therefore, we introduce the Perl script TraceHaplotyper, which works from the expected sequence to analyze a sequencing trace file for the sites that are informative for the genotyping and outputs the two underlying haplotypes.
Scientific Publication
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