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פותח על ידי קלירמאש פתרונות בע"מ -
Improved purification and molecular properties of ornithogalum mosaic virus in Israel
Year:
1998
Source of publication :
Annals of Applied Biology
Authors :
גרה, עבדאללה
;
.
ותד, עבד
;
.
Volume :
133
Co-Authors:
Zeidan, M., Department of Virology, Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Cohen, J., Department of Virology, Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Watad, A., Dept. Ornamental Horticulture, Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Gera, A., Department of Virology, Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Facilitators :
From page:
167
To page:
176
(
Total pages:
10
)
Abstract:
Symptoms associated with infection by Ornithogalum mosaic virus (OMV) were documented among horticulturally important Ornithogalum spp. grown in commercial greenhouses and open fields in Israel. OMV was mechanically transmitted to Chenopodium quinoa and C. amaranticolor. The virus was purified from infected C. quinoa using CsCI step gradient centrifugation. Virus yield of 18-24 mg kg-1 were obtained. Antiserum produced to the purified virus was highly specific in immunoblots and immunosorbent electron microscopy. Reverse transcription polymerase chain reaction (RT-PCR) utilising potyvirus-specific primers and the viral RNA resulted in the amplification of a DNA product of 1275 nucleotides. This PCR product was cloned and sequenced; it encoded an open reading frame of 333 codons and a 3' non-coding region of 275 nucleotides. Analysis of the amino acid sequences of the putative CP of the Israeli isolate of OMV showed 95% similarities to the South African isolate.
Note:
Related Files :
Israel
Oat mosaic virus
Ornithogalum
RNA viruses
sequence analysis
virus RNA
עוד תגיות
תוכן קשור
More details
DOI :
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
25837
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:17
Scientific Publication
Improved purification and molecular properties of ornithogalum mosaic virus in Israel
133
Zeidan, M., Department of Virology, Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Cohen, J., Department of Virology, Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Watad, A., Dept. Ornamental Horticulture, Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Gera, A., Department of Virology, Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Improved purification and molecular properties of ornithogalum mosaic virus in Israel
Symptoms associated with infection by Ornithogalum mosaic virus (OMV) were documented among horticulturally important Ornithogalum spp. grown in commercial greenhouses and open fields in Israel. OMV was mechanically transmitted to Chenopodium quinoa and C. amaranticolor. The virus was purified from infected C. quinoa using CsCI step gradient centrifugation. Virus yield of 18-24 mg kg-1 were obtained. Antiserum produced to the purified virus was highly specific in immunoblots and immunosorbent electron microscopy. Reverse transcription polymerase chain reaction (RT-PCR) utilising potyvirus-specific primers and the viral RNA resulted in the amplification of a DNA product of 1275 nucleotides. This PCR product was cloned and sequenced; it encoded an open reading frame of 333 codons and a 3' non-coding region of 275 nucleotides. Analysis of the amino acid sequences of the putative CP of the Israeli isolate of OMV showed 95% similarities to the South African isolate.
Scientific Publication
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