Co-Authors:
van der Wolf, J.M., Plant Research International, P.O. Box 69, 6700 AB Wageningen, Netherlands
Nijhuis, E.H., Plant Research International, P.O. Box 69, 6700 AB Wageningen, Netherlands
Kowalewska, M.J., Science and Advice for Scottish Agriculture (SASA), Edinburgh EH12 9FJ, United Kingdom
Saddler, G.S., Science and Advice for Scottish Agriculture (SASA), Edinburgh EH12 9FJ, United Kingdom
Parkinson, N., Food and Environment Research Agency (FERA), Sand Hutton, York YO41 1LZ, United Kingdom
Elphinstone, J.G., Food and Environment Research Agency (FERA), Sand Hutton, York YO41 1LZ, United Kingdom
Pritchard, L., The James Hutton Institute (JHI), Invergowrie, Dundee DD2 5DA, United Kingdom
Toth, I.K., The James Hutton Institute (JHI), Invergowrie, Dundee DD2 5DA, United Kingdom
Lojkowska, E., Kladki 24, 80-822 Gdansk, Poland
Potrykus, M., Kladki 24, 80-822 Gdansk, Poland
Waleron, M., Kladki 24, 80-822 Gdansk, Poland
de Vos, P., BCCM/LMG Bacteria Collection, Ghent University, K.L. Ledeganckstraat 35, B-90000, Ghent, Belgium
Cleenwerck, I., BCCM/LMG Bacteria Collection, Ghent University, K.L. Ledeganckstraat 35, B-90000, Ghent, Belgium
Pirhonen, M., Department of Applied Biology, Plant Pathology, University of Helsinki, 00014 Helsinki, Finland
Garlant, L., Department of Applied Biology, Plant Pathology, University of Helsinki, 00014 Helsinki, Finland
Hélias, V., National Federation of Seed Potato Growers (FN3PT) / INRA UMR 1349 IGEPP, 35653 Le Rheu Cedex, France
Pothier, J.F., Agroscope Changins-Wädenswil, Plant Protection Division, ACW, Schloss 1, 8820 Wädenswil, Switzerland, Environmental Genomics and Systems Biology, Institute of Natural Resource Sciences, Zurich University of Applied Sciences, Campus Grüental, P.O. Box CH-8820, Wädenswil, Switzerland
Pflüger, V., Mabritec AG, Lörracherstrasse 50, Postfach 320, 4125 Riehen, Switzerland
Duffy, B., Environmental Genomics and Systems Biology, Institute of Natural Resource Sciences, Zurich University of Applied Sciences, Campus Grüental, P.O. Box CH-8820, Wädenswil, Switzerland
Tsror, L., Agricultural Research Organization, Department of Plant Pathology and Weed Research, Volcani Center, Bet Dagan 50250, Israel
Manulis, S., Agricultural Research Organization, Department of Plant Pathology and Weed Research, Volcani Center, Bet Dagan 50250, Israel
Abstract:
Pectinolytic bacteria have been recently isolated from diseased potato plants exhibiting blackleg and slow wilt symptoms found in a number of European countries and Israel. These Gram-reaction-negative, motile, rods were identified as belonging to the genus Dickeya, previously the Pectobacterium chrysanthemi complex (Erwinia chrysanthemi), on the basis of production of a PCR product with the pelADE primers, 16S rRNA gene sequence analysis, fatty acid methyl esterase analysis, the production of phosphatases and the ability to produce indole and acids from α-methylglucoside. Differential physiological assays used previously to differentiate between strains of E. chrysanthemi, showed that these isolates belonged to biovar 3. Eight of the isolates, seven from potato and one from hyacinth, were analysed together with 21 reference strains representing all currently recognized taxa within the genus Dickeya. The novel isolates formed a distinct genetic clade in multilocus sequence analysis (MLSA) using concatenated sequences of the intergenic spacer (IGS), as well as dnaX, recA, dnaN, fusA, gapA, purA, rplB,rpoS and gyrA. Characterization by whole-cell MALDI-TOF mass spectrometry, pulsed field gel electrophoresis after digestion of whole-genome DNA with rare-cutting restriction enzymes, average nucleotide identity analysis and DNA-DNA hybridization studies, showed that although related to Dickeya dadantii, these isolates represent a novel species within the genus Dickeya, for which the name Dickeya solani sp. nov. (type strain IPO 2222T = LMG25993T = NCPPB4479T) is proposed. © 2014 IUMS.
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