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פותח על ידי קלירמאש פתרונות בע"מ -
Identification and characterization of hetA, a gene that acts early in the process of morphological differentiation of heterocysts
Year:
1990
Source of publication :
Journal of Bacteriology
Authors :
הולנד, דורון
;
.
Volume :
172
Co-Authors:
Holland, D., MSU-DOE Plant Research Lab., Michigan State University, East Lansing, MI 48824-1312, United States
Wolk, C.P., MSU-DOE Plant Research Lab., Michigan State University, East Lansing, MI 48824-1312, United States
Facilitators :
From page:
3131
To page:
3137
(
Total pages:
7
)
Abstract:
Envelope polysaccharide is a major early diagnostic of differentiating heterocysts. The mutation in mutant EF116 of Anabaena sp. strain PCC 7120 reduces the cohesiveness of this polysaccharide. A 3.5-kilobase fragment of DNA cloned from the wild type of this Anabaena sp. was previously shown to complement this mutation. We present the nucleotide sequence of a 2,555-base-pair portion of this fragment containing an open reading frame (ORF) of 601 amino acids. Complementation analysis using deletion derivatives of the 3.5-kilobase fragment showed that the gene mutated in EF116, which we designate hetA, lies within this ORF. Transcription of hetA was induced as a result of deprivation for nitrate and yielded a monocistronic mRNA that was present at greatest abundance 7 h after nitrogen stepdown. At that time, proheterocysts could not be distinguished by light microscopy; transription of nifHD, structural genes of nitrogenase, peaked much later. Situated 3' to hetA are 4 identical repeats of the sequence 5'-TTCAAAA-3' and 12 repeats (10 identical) of the sequence 5'-CCCCAAT-3'. The 12 repeats, present within and near the 5' end of a second ORF, are almost identical to repeats that have been reported to be present in the region between the petC and petA genes of a related cyanobacterium.
Note:
Related Files :
Base Sequence
Genetic Complementation Test
genetic engineering
Molecular Sequence Data
Repetitive Sequences, Nucleic Acid
עוד תגיות
תוכן קשור
More details
DOI :
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
25897
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:18
Scientific Publication
Identification and characterization of hetA, a gene that acts early in the process of morphological differentiation of heterocysts
172
Holland, D., MSU-DOE Plant Research Lab., Michigan State University, East Lansing, MI 48824-1312, United States
Wolk, C.P., MSU-DOE Plant Research Lab., Michigan State University, East Lansing, MI 48824-1312, United States
Identification and characterization of hetA, a gene that acts early in the process of morphological differentiation of heterocysts
Envelope polysaccharide is a major early diagnostic of differentiating heterocysts. The mutation in mutant EF116 of Anabaena sp. strain PCC 7120 reduces the cohesiveness of this polysaccharide. A 3.5-kilobase fragment of DNA cloned from the wild type of this Anabaena sp. was previously shown to complement this mutation. We present the nucleotide sequence of a 2,555-base-pair portion of this fragment containing an open reading frame (ORF) of 601 amino acids. Complementation analysis using deletion derivatives of the 3.5-kilobase fragment showed that the gene mutated in EF116, which we designate hetA, lies within this ORF. Transcription of hetA was induced as a result of deprivation for nitrate and yielded a monocistronic mRNA that was present at greatest abundance 7 h after nitrogen stepdown. At that time, proheterocysts could not be distinguished by light microscopy; transription of nifHD, structural genes of nitrogenase, peaked much later. Situated 3' to hetA are 4 identical repeats of the sequence 5'-TTCAAAA-3' and 12 repeats (10 identical) of the sequence 5'-CCCCAAT-3'. The 12 repeats, present within and near the 5' end of a second ORF, are almost identical to repeats that have been reported to be present in the region between the petC and petA genes of a related cyanobacterium.
Scientific Publication
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