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A proposed conserved role for an avocado fw2.2-like gene as a negative regulator of fruit cell division
Year:
2010
Source of publication :
Planta
Authors :
דהן, ירדנה
;
.
זדירנוב, ויקטור
;
.
יריחימוביץ', ורד
;
.
רוזנפלד, רויטל
;
.
Volume :
232
Co-Authors:
Dahan, Y., Institute of Plant Sciences, The Volcani Center, Agricultural Research Organization, 50250 Bet-Dagan, Israel
Rosenfeld, R., Institute of Plant Sciences, The Volcani Center, Agricultural Research Organization, 50250 Bet-Dagan, Israel
Zadiranov, V., Institute of Plant Sciences, The Volcani Center, Agricultural Research Organization, 50250 Bet-Dagan, Israel
Irihimovitch, V., Institute of Plant Sciences, The Volcani Center, Agricultural Research Organization, 50250 Bet-Dagan, Israel
Facilitators :
From page:
663
To page:
676
(
Total pages:
14
)
Abstract:
Previous studies using 'Hass' avocado and its small fruit (SF) phenotype as a model showed that SF is limited by cell number, not by cell size. In an attempt to explore the molecular mechanisms regulating avocado fruit cell division, we isolated four distinct avocado cell proliferation-related genes and investigated their expression characteristics, comparing normal fruit (NF) and SF developmental patterns. Three cDNAs termed PaCYCA1, PaCYCB1 and PaPCNA, encoding two mitotic cyclins and a proliferating cell nuclear antigen (PCNA), were first isolated from young NF tissues. The accumulation of their transcripts was predominant in mitotically active organs, including young fruitlets, leaves and roots. Furthermore, a fourth full-length cDNA, designated Pafw2.2-like, encoding a FW2.2 (fruit-weight)-like protein, was isolated from SF tissues. FW2.2 is postulated to function as a negative regulator of cell division in tomato fruit. Remarkably, northern analysis revealed that the accumulation of the mitotic cyclins and of PCNA transcripts gradually decreased in NF tissues during growth, whereas in SF, their levels had already decreased at earlier stages of fruit development, concomitant with an earlier arrest of fruit cell division activity. In contrast, parallel sq-RT-PCR analysis showed that Pafw2.2-like mRNA accumulation was considerably higher in SF tissues than in the same NF tissues essentially at all examined stages of fruit growth. Together, our data suggest essential roles for the two mitotic cyclins genes and the PCNA gene in regulating avocado fruit development. Furthermore, the possibility that Pafw2.2-like acts as does fw2.2 in tomato, is discussed. © 2010 Springer-Verlag.
Note:
Related Files :
Avocado
Base Sequence
biosynthesis
Cell Division
Genetics
Persea americana
reverse transcription polymerase chain reaction
עוד תגיות
תוכן קשור
More details
DOI :
10.1007/s00425-010-1200-3
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
25975
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:19
You may also be interested in
Scientific Publication
A proposed conserved role for an avocado fw2.2-like gene as a negative regulator of fruit cell division
232
Dahan, Y., Institute of Plant Sciences, The Volcani Center, Agricultural Research Organization, 50250 Bet-Dagan, Israel
Rosenfeld, R., Institute of Plant Sciences, The Volcani Center, Agricultural Research Organization, 50250 Bet-Dagan, Israel
Zadiranov, V., Institute of Plant Sciences, The Volcani Center, Agricultural Research Organization, 50250 Bet-Dagan, Israel
Irihimovitch, V., Institute of Plant Sciences, The Volcani Center, Agricultural Research Organization, 50250 Bet-Dagan, Israel
A proposed conserved role for an avocado fw2.2-like gene as a negative regulator of fruit cell division
Previous studies using 'Hass' avocado and its small fruit (SF) phenotype as a model showed that SF is limited by cell number, not by cell size. In an attempt to explore the molecular mechanisms regulating avocado fruit cell division, we isolated four distinct avocado cell proliferation-related genes and investigated their expression characteristics, comparing normal fruit (NF) and SF developmental patterns. Three cDNAs termed PaCYCA1, PaCYCB1 and PaPCNA, encoding two mitotic cyclins and a proliferating cell nuclear antigen (PCNA), were first isolated from young NF tissues. The accumulation of their transcripts was predominant in mitotically active organs, including young fruitlets, leaves and roots. Furthermore, a fourth full-length cDNA, designated Pafw2.2-like, encoding a FW2.2 (fruit-weight)-like protein, was isolated from SF tissues. FW2.2 is postulated to function as a negative regulator of cell division in tomato fruit. Remarkably, northern analysis revealed that the accumulation of the mitotic cyclins and of PCNA transcripts gradually decreased in NF tissues during growth, whereas in SF, their levels had already decreased at earlier stages of fruit development, concomitant with an earlier arrest of fruit cell division activity. In contrast, parallel sq-RT-PCR analysis showed that Pafw2.2-like mRNA accumulation was considerably higher in SF tissues than in the same NF tissues essentially at all examined stages of fruit growth. Together, our data suggest essential roles for the two mitotic cyclins genes and the PCNA gene in regulating avocado fruit development. Furthermore, the possibility that Pafw2.2-like acts as does fw2.2 in tomato, is discussed. © 2010 Springer-Verlag.
Scientific Publication
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