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פותח על ידי קלירמאש פתרונות בע"מ -
Involvement of a subgenomic mRNA in the generation of a variable population of defective citrus tristeza virus molecules
Year:
1997
Source of publication :
Journal of Virology
Authors :
בר-יוסף, משה
;
.
גופמן, רוז
;
.
גפני, רון
;
.
יאנג, גואנג
;
.
מוואסי, מוניר
;
.
Volume :
71
Co-Authors:
Yang, G., S. Tolkowsky Laboratory, Dept. of Virology Agrl. Res. Org., Bet Dagan, Israel
Mawassi, M., S. Tolkowsky Laboratory, Dept. of Virology Agrl. Res. Org., Bet Dagan, Israel
Gofman, R., S. Tolkowsky Laboratory, Dept. of Virology Agrl. Res. Org., Bet Dagan, Israel
Gafny, R., S. Tolkowsky Laboratory, Dept. of Virology Agrl. Res. Org., Bet Dagan, Israel
Bar-Joseph, M., S. Tolkowsky Laboratory, Dept. of Virology Agrl. Res. Org., Bet Dagan, Israel
Facilitators :
From page:
9800
To page:
9802
(
Total pages:
3
)
Abstract:
The fusion sites between the termini of naturally occurring defective RNAs (D-RNAs) from three citrus tristeza virus (CTV) isolates were sequenced. Seven of eight clones showed a common 3' terminus of 940 nucleotides (nt) fused to 5' termini with different sizes. An extra cytosine nucleotide was found at the junction site of the majority of the common 3' D-RNAs. Molecular analysis of the plus and minus strands of the 0.9-kbp double-stranded RNA, corresponding to the CTV open reading frame 11 subgenomic RNA (sgRNA), showed that they were identical in length and sequence to the common 3' sequence of the D-RNAs. These results imply that viral sgRNA messengers also function as building components for genomic rearrangement and exchange of complete viral genes.
Note:
Related Files :
Artificial Gene Fusion
double stranded DNA
genetic analysis
gene transfer
sequence analysis
virus gene
עוד תגיות
תוכן קשור
More details
DOI :
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
26018
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:19
Scientific Publication
Involvement of a subgenomic mRNA in the generation of a variable population of defective citrus tristeza virus molecules
71
Yang, G., S. Tolkowsky Laboratory, Dept. of Virology Agrl. Res. Org., Bet Dagan, Israel
Mawassi, M., S. Tolkowsky Laboratory, Dept. of Virology Agrl. Res. Org., Bet Dagan, Israel
Gofman, R., S. Tolkowsky Laboratory, Dept. of Virology Agrl. Res. Org., Bet Dagan, Israel
Gafny, R., S. Tolkowsky Laboratory, Dept. of Virology Agrl. Res. Org., Bet Dagan, Israel
Bar-Joseph, M., S. Tolkowsky Laboratory, Dept. of Virology Agrl. Res. Org., Bet Dagan, Israel
Involvement of a subgenomic mRNA in the generation of a variable population of defective citrus tristeza virus molecules
The fusion sites between the termini of naturally occurring defective RNAs (D-RNAs) from three citrus tristeza virus (CTV) isolates were sequenced. Seven of eight clones showed a common 3' terminus of 940 nucleotides (nt) fused to 5' termini with different sizes. An extra cytosine nucleotide was found at the junction site of the majority of the common 3' D-RNAs. Molecular analysis of the plus and minus strands of the 0.9-kbp double-stranded RNA, corresponding to the CTV open reading frame 11 subgenomic RNA (sgRNA), showed that they were identical in length and sequence to the common 3' sequence of the D-RNAs. These results imply that viral sgRNA messengers also function as building components for genomic rearrangement and exchange of complete viral genes.
Scientific Publication
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