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אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Development of broad virus resistance in non-transgenic cucumber using CRISPR/Cas9 technology
Year:
2016
Source of publication :
Molecular Plant Pathology
Authors :
ארזי, צחי
;
.
ברומין, מרינה
;
.
גל-און, עמית
;
.
וולף, דליה
;
.
ליבמן, דיאנה
;
.
פרלסמן, מלניה
;
.
צ'נדראסקראן, ג'יאבאראטי
;
.
קלפ, חן
;
.
שרמן, עמיר
;
.
Volume :
17
Co-Authors:

Chandrasekaran, J., Department of Plant Pathology and Weed Research, ARO, Volcani Center, Bet-Dagan, Israel
Brumin, M., Department of Plant Pathology and Weed Research, ARO, Volcani Center, Bet-Dagan, Israel
Wolf, D., Department of Vegetable Research, ARO, Volcani Center, Bet-Dagan, Israel
Leibman, D., Department of Plant Pathology and Weed Research, ARO, Volcani Center, Bet-Dagan, Israel
Klap, C., Department of Plant Pathology and Weed Research, ARO, Volcani Center, Bet-Dagan, Israel
Pearlsman, M., Department of Plant Pathology and Weed Research, ARO, Volcani Center, Bet-Dagan, Israel
Sherman, A., Department of Fruit Tree Sciences, ARO, Volcani Center, Bet-Dagan, Israel
Arazi, T., Department of Ornamental Plants and Agricultural Biotechnology, ARO, Volcani Center, Bet-Dagan, Israel
Gal-On, A., Department of Plant Pathology and Weed Research, ARO, Volcani Center, Bet-Dagan, Israel
 

Facilitators :
From page:
1140
To page:
1153
(
Total pages:
14
)
Abstract:
Genome editing in plants has been boosted tremendously by the development of CRISPR/Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats) technology. This powerful tool allows substantial improvement in plant traits in addition to those provided by classical breeding. Here, we demonstrate the development of virus resistance in cucumber (Cucumis sativus L.) using Cas9/subgenomic RNA (sgRNA) technology to disrupt the function of the recessive eIF4E (eukaryotic translation initiation factor 4E) gene. Cas9/sgRNA constructs were targeted to the N′ and C′ termini of the eIF4E gene. Small deletions and single nucleotide polymorphisms (SNPs) were observed in the eIF4E gene targeted sites of transformed T1 generation cucumber plants, but not in putative off-target sites. Non-transgenic heterozygous eif4e mutant plants were selected for the production of non-transgenic homozygous T3 generation plants. Homozygous T3 progeny following Cas9/sgRNA that had been targeted to both eif4e sites exhibited immunity to Cucumber vein yellowing virus (Ipomovirus) infection and resistance to the potyviruses Zucchini yellow mosaic virus and Papaya ring spot mosaic virus-W. In contrast, heterozygous mutant and non-mutant plants were highly susceptible to these viruses. For the first time, virus resistance has been developed in cucumber, non-transgenically, not visibly affecting plant development and without long-term backcrossing, via a new technology that can be expected to be applicable to a wide range of crop plants. © 2016 BSPP and John Wiley & Sons Ltd
Note:
Related Files :
Cucumis sativus
disease resistance
Genetics
mutation
Plant Disease
virus resistance
עוד תגיות
תוכן קשור
More details
DOI :
10.1111/mpp.12375
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
26065
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:19
You may also be interested in
Scientific Publication
Development of broad virus resistance in non-transgenic cucumber using CRISPR/Cas9 technology
17

Chandrasekaran, J., Department of Plant Pathology and Weed Research, ARO, Volcani Center, Bet-Dagan, Israel
Brumin, M., Department of Plant Pathology and Weed Research, ARO, Volcani Center, Bet-Dagan, Israel
Wolf, D., Department of Vegetable Research, ARO, Volcani Center, Bet-Dagan, Israel
Leibman, D., Department of Plant Pathology and Weed Research, ARO, Volcani Center, Bet-Dagan, Israel
Klap, C., Department of Plant Pathology and Weed Research, ARO, Volcani Center, Bet-Dagan, Israel
Pearlsman, M., Department of Plant Pathology and Weed Research, ARO, Volcani Center, Bet-Dagan, Israel
Sherman, A., Department of Fruit Tree Sciences, ARO, Volcani Center, Bet-Dagan, Israel
Arazi, T., Department of Ornamental Plants and Agricultural Biotechnology, ARO, Volcani Center, Bet-Dagan, Israel
Gal-On, A., Department of Plant Pathology and Weed Research, ARO, Volcani Center, Bet-Dagan, Israel
 

Development of broad virus resistance in non-transgenic cucumber using CRISPR/Cas9 technology
Genome editing in plants has been boosted tremendously by the development of CRISPR/Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats) technology. This powerful tool allows substantial improvement in plant traits in addition to those provided by classical breeding. Here, we demonstrate the development of virus resistance in cucumber (Cucumis sativus L.) using Cas9/subgenomic RNA (sgRNA) technology to disrupt the function of the recessive eIF4E (eukaryotic translation initiation factor 4E) gene. Cas9/sgRNA constructs were targeted to the N′ and C′ termini of the eIF4E gene. Small deletions and single nucleotide polymorphisms (SNPs) were observed in the eIF4E gene targeted sites of transformed T1 generation cucumber plants, but not in putative off-target sites. Non-transgenic heterozygous eif4e mutant plants were selected for the production of non-transgenic homozygous T3 generation plants. Homozygous T3 progeny following Cas9/sgRNA that had been targeted to both eif4e sites exhibited immunity to Cucumber vein yellowing virus (Ipomovirus) infection and resistance to the potyviruses Zucchini yellow mosaic virus and Papaya ring spot mosaic virus-W. In contrast, heterozygous mutant and non-mutant plants were highly susceptible to these viruses. For the first time, virus resistance has been developed in cucumber, non-transgenically, not visibly affecting plant development and without long-term backcrossing, via a new technology that can be expected to be applicable to a wide range of crop plants. © 2016 BSPP and John Wiley & Sons Ltd
Scientific Publication
You may also be interested in