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פותח על ידי קלירמאש פתרונות בע"מ -
Bovine pituitary, kidney, uterine and mammary gland extracts contain bovine mammary epithelium growth factors that synergise with IGF-I and fetal calf serum: Indication for involvement of GTP-binding proteins
Year:
1991
Source of publication :
Domestic Animal Endocrinology
Authors :
שמאי, אבי
;
.
Volume :
8
Co-Authors:
Waksman, M., Department of Biochemistry, Human Nutrition Faculty of Agriculture, The Hebrew University of Jerusalem, Rehovot, 76100, Israel
Shamay, A., Department of Biochemistry, Human Nutrition Faculty of Agriculture, The Hebrew University of Jerusalem, Rehovot, 76100, Israel
Gertler, A., Department of Biochemistry, Human Nutrition Faculty of Agriculture, The Hebrew University of Jerusalem, Rehovot, 76100, Israel
Facilitators :
From page:
271
To page:
280
(
Total pages:
10
)
Abstract:
Bovine mammary undifferentiated epithelial cells from young female calves, cultured in three-dimensional collagen gels in serum-free medium exhibited ultrastructural organization that resembled the in vivo situation. Extracts of bovine pituitary, kidney, uterus and mammary gland, stimulated cell proliferation in a dose-dependent manner. This mitogenic activity strongly synergised with the existant growth factors (GFs) in FCS and with IGF-I, while the addition of EGF had only minor effect. No synergistic manifestation was found with cholera toxin but pertussis toxin inhibited the growth-promoting activity of all four extracts. Other experiments indicated that this mitogenic activity does not result from prolactin, growth hormone or fibroblast growth factor. The present and former results, in which synergism between IGF-I and cholera toxin was demonstrated, suggest therefore, that the mitogenesis of normal mammary epithelial cells regulated by several tissue derived growth factors, consists of at least two pathways which are distinct from those activated by EGF and IGF-I. One of these pathways indicates involvement of pertussis toxin-sensitive GTP-binding proteins, and the other, activation of cholera toxin-sensitive adenylate cyclase. © 1991.
Note:
Related Files :
Animal
cattle
Cell Division
chemistry
drug effect
epithelium cell
Female
Insulin-Like Growth Factor I
ultrastructure
עוד תגיות
תוכן קשור
More details
DOI :
10.1016/0739-7240(91)90063-P
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
26086
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:20
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Scientific Publication
Bovine pituitary, kidney, uterine and mammary gland extracts contain bovine mammary epithelium growth factors that synergise with IGF-I and fetal calf serum: Indication for involvement of GTP-binding proteins
8
Waksman, M., Department of Biochemistry, Human Nutrition Faculty of Agriculture, The Hebrew University of Jerusalem, Rehovot, 76100, Israel
Shamay, A., Department of Biochemistry, Human Nutrition Faculty of Agriculture, The Hebrew University of Jerusalem, Rehovot, 76100, Israel
Gertler, A., Department of Biochemistry, Human Nutrition Faculty of Agriculture, The Hebrew University of Jerusalem, Rehovot, 76100, Israel
Bovine pituitary, kidney, uterine and mammary gland extracts contain bovine mammary epithelium growth factors that synergise with IGF-I and fetal calf serum: Indication for involvement of GTP-binding proteins
Bovine mammary undifferentiated epithelial cells from young female calves, cultured in three-dimensional collagen gels in serum-free medium exhibited ultrastructural organization that resembled the in vivo situation. Extracts of bovine pituitary, kidney, uterus and mammary gland, stimulated cell proliferation in a dose-dependent manner. This mitogenic activity strongly synergised with the existant growth factors (GFs) in FCS and with IGF-I, while the addition of EGF had only minor effect. No synergistic manifestation was found with cholera toxin but pertussis toxin inhibited the growth-promoting activity of all four extracts. Other experiments indicated that this mitogenic activity does not result from prolactin, growth hormone or fibroblast growth factor. The present and former results, in which synergism between IGF-I and cholera toxin was demonstrated, suggest therefore, that the mitogenesis of normal mammary epithelial cells regulated by several tissue derived growth factors, consists of at least two pathways which are distinct from those activated by EGF and IGF-I. One of these pathways indicates involvement of pertussis toxin-sensitive GTP-binding proteins, and the other, activation of cholera toxin-sensitive adenylate cyclase. © 1991.
Scientific Publication
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