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פותח על ידי קלירמאש פתרונות בע"מ -
Memory and long-term potentiation (LTP) dissociated: Normal spatial memory despite CA1 LTP elimination with Kv1.4 antisense
Year:
1998
Authors :
מאירי, נעם
;
.
Volume :
95
Co-Authors:
Meiri, N., Laboratory of Adaptive Systems, National Institutes of Health, Bethesda, MD 20892, United States
Sun, M.-K., Laboratory of Adaptive Systems, National Institutes of Health, Bethesda, MD 20892, United States
Segal, Z., Laboratory of Adaptive Systems, National Institutes of Health, Bethesda, MD 20892, United States
Alkon, D.L., Laboratory of Adaptive Systems, National Institutes of Health, Bethesda, MD 20892, United States, Natl. Inst. Neurol. Disord. Stroke, Laboratory of Adaptive Systems, MSC 4124, 36 Convent Drive, Bethesda, MD 20892-4124, United States
Facilitators :
From page:
15037
To page:
15042
(
Total pages:
6
)
Abstract:
Long-term potentiation (LTP) in the hippocampal slice preparation has been proposed as an in vitro model for long-term memory. However, correlation of LTP with memory in living animals has been difficult to demonstrate. Furthermore, in the last few years evidence has accumulated that dissociate the two. Because potassium channels might determine the weight of synapses in networks, we studied the role of Kv1.4, a presynaptic A-type voltage- dependent K+ channel, in both memory and LTP. Reverse transcription-PCR and Western blot analysis with specific antibodies showed that antisense oligodeoxyribonucleotide to Kv1.4 microinjected intraventricularly into rat brains obstructed hippocampal Kv1.4 mRNA, 'knocking down' the protein in the hippocampus. This antisense knockdown had no effect on rat spatial maze learning, memory, or exploratory behavior, but eliminated both early- and late-phase LTP and reduced paired-pulse facilitation (a presynaptic effect) in CA1 pyramidal neurons without affecting dentate gyrus LTP. This presynaptic Kv1.4 knockdown together with previous postsynaptic Kv1.1 knockdown demonstrates that CA1 LTP is neither necessary nor sufficient for rat spatial memory.
Note:
Related Files :
animal experiment
Animals
animal tissue
long term memory
Male
potassium ion
reverse transcription polymerase chain reaction
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תוכן קשור
More details
DOI :
10.1073/pnas.95.25.15037
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
26164
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:20
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Scientific Publication
Memory and long-term potentiation (LTP) dissociated: Normal spatial memory despite CA1 LTP elimination with Kv1.4 antisense
95
Meiri, N., Laboratory of Adaptive Systems, National Institutes of Health, Bethesda, MD 20892, United States
Sun, M.-K., Laboratory of Adaptive Systems, National Institutes of Health, Bethesda, MD 20892, United States
Segal, Z., Laboratory of Adaptive Systems, National Institutes of Health, Bethesda, MD 20892, United States
Alkon, D.L., Laboratory of Adaptive Systems, National Institutes of Health, Bethesda, MD 20892, United States, Natl. Inst. Neurol. Disord. Stroke, Laboratory of Adaptive Systems, MSC 4124, 36 Convent Drive, Bethesda, MD 20892-4124, United States
Memory and long-term potentiation (LTP) dissociated: Normal spatial memory despite CA1 LTP elimination with Kv1.4 antisense
Long-term potentiation (LTP) in the hippocampal slice preparation has been proposed as an in vitro model for long-term memory. However, correlation of LTP with memory in living animals has been difficult to demonstrate. Furthermore, in the last few years evidence has accumulated that dissociate the two. Because potassium channels might determine the weight of synapses in networks, we studied the role of Kv1.4, a presynaptic A-type voltage- dependent K+ channel, in both memory and LTP. Reverse transcription-PCR and Western blot analysis with specific antibodies showed that antisense oligodeoxyribonucleotide to Kv1.4 microinjected intraventricularly into rat brains obstructed hippocampal Kv1.4 mRNA, 'knocking down' the protein in the hippocampus. This antisense knockdown had no effect on rat spatial maze learning, memory, or exploratory behavior, but eliminated both early- and late-phase LTP and reduced paired-pulse facilitation (a presynaptic effect) in CA1 pyramidal neurons without affecting dentate gyrus LTP. This presynaptic Kv1.4 knockdown together with previous postsynaptic Kv1.1 knockdown demonstrates that CA1 LTP is neither necessary nor sufficient for rat spatial memory.
Scientific Publication
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