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פותח על ידי קלירמאש פתרונות בע"מ -
Absorption of oleic and taurocholic acids from the intestine of the chick. Interactions and interference by proteins
Year:
1979
Authors :
הורביץ, שמואל
;
.
Volume :
573
Co-Authors:
Sklan, D., Department of Animal Science, Faculty of Agriculture, The Hebrew University of Jerusalem, P.O.Box 12, RehovotIsrael
Budowski, P., Department of Animal Science, Faculty of Agriculture, The Hebrew University of Jerusalem, P.O.Box 12, RehovotIsrael
Hurwitz, S., Department of Animal Science, Faculty of Agriculture, The Hebrew University of Jerusalem, P.O.Box 12, RehovotIsrael
Facilitators :
From page:
31
To page:
39
(
Total pages:
9
)
Abstract:
The interaction between fatty acids, bile acids and proteins in the intestinal lumen and its effect on the mucosal uptake of fatty acids and bile acids in chicks were studied in situ by means of ligated duodenal loops. The basal medium introduced into the loops contained oleic and taurocholic acids in bicarbonate buffer at pH 6.5, with trace amounts of [14C]oleic acid and [14C]-taurocholic acid, [3H]dextran serving as a nonabsorbable reference substance. Tests conducted with varying initial concentrations of oleic acid and taurocholic acid pointed to an optimal molar ratio of oleic acid to taurocholic acid at which the rates of mucosal uptake of both compounds were highest and numerically equal. Addition of casein, albumin or soy protein to the medium caused considerable inhibition of both oleic acid and taurocholic acid uptake. Taurocholic acid uptake alone, or that of the slightly water-soluble lauric acid alone, was similarly decreased by protein. Predigestion of the proteins with pepsin A (EC 3.4.23.1) or elastase (EC 3.4.21.11) eliminated the inhibitory effect on uptake of oleic acid and taurocholic acid. It was shown that casein is able to bind both oleic acid and taurocholic acid. Thus the presence of undigested protein in the upper small intestine of the chick may interfere with the mucosal uptake of lipids by a double action involving direct binding of fatty acids to the protein and disturbance of micellar solubilization of the lipids because of binding of bile acids. © 1979.
Note:
Related Files :
(Chick intestine)
Animal
animal experiment
Caseins
Chickens
Protein interaction
Protein interference
proteins
Taurocholic Acid
עוד תגיות
תוכן קשור
More details
DOI :
10.1016/0005-2760(79)90170-X
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
26168
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:20
Scientific Publication
Absorption of oleic and taurocholic acids from the intestine of the chick. Interactions and interference by proteins
573
Sklan, D., Department of Animal Science, Faculty of Agriculture, The Hebrew University of Jerusalem, P.O.Box 12, RehovotIsrael
Budowski, P., Department of Animal Science, Faculty of Agriculture, The Hebrew University of Jerusalem, P.O.Box 12, RehovotIsrael
Hurwitz, S., Department of Animal Science, Faculty of Agriculture, The Hebrew University of Jerusalem, P.O.Box 12, RehovotIsrael
Absorption of oleic and taurocholic acids from the intestine of the chick. Interactions and interference by proteins
The interaction between fatty acids, bile acids and proteins in the intestinal lumen and its effect on the mucosal uptake of fatty acids and bile acids in chicks were studied in situ by means of ligated duodenal loops. The basal medium introduced into the loops contained oleic and taurocholic acids in bicarbonate buffer at pH 6.5, with trace amounts of [14C]oleic acid and [14C]-taurocholic acid, [3H]dextran serving as a nonabsorbable reference substance. Tests conducted with varying initial concentrations of oleic acid and taurocholic acid pointed to an optimal molar ratio of oleic acid to taurocholic acid at which the rates of mucosal uptake of both compounds were highest and numerically equal. Addition of casein, albumin or soy protein to the medium caused considerable inhibition of both oleic acid and taurocholic acid uptake. Taurocholic acid uptake alone, or that of the slightly water-soluble lauric acid alone, was similarly decreased by protein. Predigestion of the proteins with pepsin A (EC 3.4.23.1) or elastase (EC 3.4.21.11) eliminated the inhibitory effect on uptake of oleic acid and taurocholic acid. It was shown that casein is able to bind both oleic acid and taurocholic acid. Thus the presence of undigested protein in the upper small intestine of the chick may interfere with the mucosal uptake of lipids by a double action involving direct binding of fatty acids to the protein and disturbance of micellar solubilization of the lipids because of binding of bile acids. © 1979.
Scientific Publication
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