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פותח על ידי קלירמאש פתרונות בע"מ -
Transgenes consisting of a dsRNA of an RNAi suppressor plus the 3′ UTR provide resistance to Citrus tristeza virus sequences in Nicotiana benthamiana but not in citrus
Year:
2006
Source of publication :
Virus Genes
Authors :
באטומן, אוזגור
;
.
בר-יוסף, משה
;
.
מוואסי, מוניר
;
.
Volume :
33
Co-Authors:
Batuman, O., S. Tolkowsky Laboratory, Virology Department, Volcani Center, Bet Dagan 50250, Israel
Mawassi, M., S. Tolkowsky Laboratory, Virology Department, Volcani Center, Bet Dagan 50250, Israel
Bar-Joseph, M., S. Tolkowsky Laboratory, Virology Department, Volcani Center, Bet Dagan 50250, Israel
Facilitators :
From page:
319
To page:
327
(
Total pages:
9
)
Abstract:
In an attempt to utilize post-transcriptional gene silencing (PTGS) as a means to impart resistance against Citrus tristeza virus (CTV) into citrus plants, the p23 + 3′UTR sequence (p23U) of the VT strain of CTV was engineered to fold into a double-stranded (ds) RNA structure. The resulting construct (p23UI) was introduced into Nicotiana benthamiana and Alemow (Citrus macrophylla) plants by Agrobacterium-mediated transformation. Transgenic p23UI- N. benthamiana were resistant to infection with a viral vector made of Grapevine virus A (GVA) + p23U (GVA-p23U), as indicated by the absence of the chimeric virus from inoculated plants. Inoculation of transgenic p23UI Alemow plants with CTV resulted in delayed appearance of symptoms in 9 out of the 70 transgenic plants. However, none of the plants showed durable resistance, as indicated by the obtaining of similar Northern hybridization signals from both transgenic and non-transgenic citrus plants. The possible causes for the failure of transgenic citrus plants to confer durable resistance to CTV are discussed. © Springer Science+Business Media, LLC 2006.
Note:
Related Files :
Agrobacterium
Citrus tristeza virus
gene silencing
genetic engineering
genetic transformation
transgenic plants
virus resistance
עוד תגיות
תוכן קשור
More details
DOI :
10.1007/s11262-006-0071-y
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
26226
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:21
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Scientific Publication
Transgenes consisting of a dsRNA of an RNAi suppressor plus the 3′ UTR provide resistance to Citrus tristeza virus sequences in Nicotiana benthamiana but not in citrus
33
Batuman, O., S. Tolkowsky Laboratory, Virology Department, Volcani Center, Bet Dagan 50250, Israel
Mawassi, M., S. Tolkowsky Laboratory, Virology Department, Volcani Center, Bet Dagan 50250, Israel
Bar-Joseph, M., S. Tolkowsky Laboratory, Virology Department, Volcani Center, Bet Dagan 50250, Israel
Transgenes consisting of a dsRNA of an RNAi suppressor plus the 3′ UTR provide resistance to Citrus tristeza virus sequences in Nicotiana benthamiana but not in citrus
In an attempt to utilize post-transcriptional gene silencing (PTGS) as a means to impart resistance against Citrus tristeza virus (CTV) into citrus plants, the p23 + 3′UTR sequence (p23U) of the VT strain of CTV was engineered to fold into a double-stranded (ds) RNA structure. The resulting construct (p23UI) was introduced into Nicotiana benthamiana and Alemow (Citrus macrophylla) plants by Agrobacterium-mediated transformation. Transgenic p23UI- N. benthamiana were resistant to infection with a viral vector made of Grapevine virus A (GVA) + p23U (GVA-p23U), as indicated by the absence of the chimeric virus from inoculated plants. Inoculation of transgenic p23UI Alemow plants with CTV resulted in delayed appearance of symptoms in 9 out of the 70 transgenic plants. However, none of the plants showed durable resistance, as indicated by the obtaining of similar Northern hybridization signals from both transgenic and non-transgenic citrus plants. The possible causes for the failure of transgenic citrus plants to confer durable resistance to CTV are discussed. © Springer Science+Business Media, LLC 2006.
Scientific Publication
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