חיפוש מתקדם
Suliman-Pollatschek, S., ARO-Volcani Center, P. O. Box 6, Bet-Dagan 50250, Israel
Kashkush, K., ARO-Volcani Center, P. O. Box 6, Bet-Dagan 50250, Israel
Shats, H., Faculty of Agriculture, Hebrew University, P. O. Box 12, Rehovot 76100, Israel, MBC, Science Park Rehovot, 5 Oppenheimer St. P.O.B 4018, Nes- Ziona 70400, Israel
Hillel, J., Department of Plant Sciences, Weizmann Institute, Rehovot 76100, Israel
Lavi, U., ARO-Volcani Center, P. O. Box 6, Bet-Dagan 50250, Israel
Amplified Fragment Length Polymorphism (AFLP), Simple Sequence Repeat (SSR) and Single Nucleotide Polymorphism (SNP), were applied to the tomato genome for assessment of polymorphism and for mapping. The polymorphism of AFLP was studied in twenty-one commercial tomato (L. esculentum) varieties. Four AFLP primer combinations produced 298 clear bands; an average of 75 bands per combination. SSR markers were generated from two sources: (1) size-selected genomic libraries screened with (AT)n, (CT)n, (GT)n, (ATT)n and (CTT)n probes. (2) GeneBank database. Primers were designed for 114 loci and used for genotyping 13 tomato varieties and three Lycopersicon species. Eighteen markers were used to evaluate the polymorphism among the commercial cultivars and were found to be a useful tool for cultivar identification. In-silico Comparison of DNA sequences (ESTs and genes) of L. pennellii and L. esculentum, yielded 312 SNPs. Ten L. pennelli genomic fragments were sequenced and the comparison with L. esculentum yielded 22 SNPs: Another 19 SNPs were discovered by sequencing and comparing L. pennellii genomic DNA to L. esculentum DNA fragments containing SSRs. The average SNP frequency was found to be one in a few tens of base pairs. A total of 52 microsatellites, 159 polymorphic AFLP markers and six SNPs were mapped using the Introgression Lines generated by [1]. Map location and markers' distribution are presented.
פותח על ידי קלירמאש פתרונות בע"מ -
הספר "אוצר וולקני"
אודות
תנאי שימוש
Generation and mapping of AFLP, SSRs and SNPs in Lycopersicon esculentum
7
Suliman-Pollatschek, S., ARO-Volcani Center, P. O. Box 6, Bet-Dagan 50250, Israel
Kashkush, K., ARO-Volcani Center, P. O. Box 6, Bet-Dagan 50250, Israel
Shats, H., Faculty of Agriculture, Hebrew University, P. O. Box 12, Rehovot 76100, Israel, MBC, Science Park Rehovot, 5 Oppenheimer St. P.O.B 4018, Nes- Ziona 70400, Israel
Hillel, J., Department of Plant Sciences, Weizmann Institute, Rehovot 76100, Israel
Lavi, U., ARO-Volcani Center, P. O. Box 6, Bet-Dagan 50250, Israel
Generation and mapping of AFLP, SSRs and SNPs in Lycopersicon esculentum
Amplified Fragment Length Polymorphism (AFLP), Simple Sequence Repeat (SSR) and Single Nucleotide Polymorphism (SNP), were applied to the tomato genome for assessment of polymorphism and for mapping. The polymorphism of AFLP was studied in twenty-one commercial tomato (L. esculentum) varieties. Four AFLP primer combinations produced 298 clear bands; an average of 75 bands per combination. SSR markers were generated from two sources: (1) size-selected genomic libraries screened with (AT)n, (CT)n, (GT)n, (ATT)n and (CTT)n probes. (2) GeneBank database. Primers were designed for 114 loci and used for genotyping 13 tomato varieties and three Lycopersicon species. Eighteen markers were used to evaluate the polymorphism among the commercial cultivars and were found to be a useful tool for cultivar identification. In-silico Comparison of DNA sequences (ESTs and genes) of L. pennellii and L. esculentum, yielded 312 SNPs. Ten L. pennelli genomic fragments were sequenced and the comparison with L. esculentum yielded 22 SNPs: Another 19 SNPs were discovered by sequencing and comparing L. pennellii genomic DNA to L. esculentum DNA fragments containing SSRs. The average SNP frequency was found to be one in a few tens of base pairs. A total of 52 microsatellites, 159 polymorphic AFLP markers and six SNPs were mapped using the Introgression Lines generated by [1]. Map location and markers' distribution are presented.
Scientific Publication
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