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פותח על ידי קלירמאש פתרונות בע"מ -
Cellular and molecular changes associated with onion skin formation suggest involvement of programmed cell death
Year:
2017
Source of publication :
Frontiers in Plant Science
Authors :
אשל, דני
;
.
גלסורקר, אורטל
;
.
דורון-פייגנבאום, עדי
;
.
דעוס, אבינועם
;
.
טפר-במנולקר, פאולה
;
.
לרס, אמנון
;
.
Volume :
7
Co-Authors:

Fridman, Y., The Alexander Silberman Institute of Life Science, Edmond Safra Campus (G Ram), The Hebrew University, Jerusalem, Israel
 

Facilitators :
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0
To page:
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Total pages:
1
)
Abstract:
Skin formation of onion (Allium cepa L.) bulb involves scale desiccation accompanied by scale senescence, resulting in cell death and tissue browning. Understanding the mechanism of skin formation is essential to improving onion skin and bulb qualities. Although onion skin plays a crucial role in postharvest onion storage and shelf life, its formation is poorly understood. We investigated the mode of cell death in the outermost scales that are destined to form the onion skin. Surprisingly, fluorescein diacetate staining and scanning electron microscopy indicated that the outer scale desiccates from the inside out. This striking observation suggests that cell death in the outer scales, during skin formation, is an internal and organized process that does not derive only from air desiccation. DNA fragmentation, a known hallmark of programmed cell death (PCD), was revealed in the outer scales and gradually decreased toward the inner scales of the bulb. Transmission electron microscopy further revealed PCD-related structural alterations in the outer scales which were absent from the inner scales. De novo transcriptome assembly for three different scales: 1st (outer), 5th (intermediate) and 8th (inner) fleshy scales identified 2,542 differentially expressed genes among them. GO enrichment for cluster analysis revealed increasing metabolic processes in the outer senescent scale related to defense response, PCD processes, carbohydrate metabolism and flavonoid biosynthesis, whereas increased metabolism and developmental growth processes were identified in the inner scales. High expression levels of PCD-related genes were identified in the outer scale compared to the inner ones, highlighting the involvement of PCD in outer-skin development. These findings suggest that a program to form the dry protective skin exists and functions only in the outer scales of onion. © 2017 Galsurker, Doron-Faigenboim, Teper-Bamnolker, Daus, Fridman, Lers and Eshel.
Note:
Related Files :
DNA fragmentation
Onion-skin
programmed cell death
scale
transcriptome
עוד תגיות
תוכן קשור
More details
DOI :
10.3389/fpls.2016.02031
Article number:
2031
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
26263
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:21
Scientific Publication
Cellular and molecular changes associated with onion skin formation suggest involvement of programmed cell death
7

Fridman, Y., The Alexander Silberman Institute of Life Science, Edmond Safra Campus (G Ram), The Hebrew University, Jerusalem, Israel
 

Cellular and molecular changes associated with onion skin formation suggest involvement of programmed cell death
Skin formation of onion (Allium cepa L.) bulb involves scale desiccation accompanied by scale senescence, resulting in cell death and tissue browning. Understanding the mechanism of skin formation is essential to improving onion skin and bulb qualities. Although onion skin plays a crucial role in postharvest onion storage and shelf life, its formation is poorly understood. We investigated the mode of cell death in the outermost scales that are destined to form the onion skin. Surprisingly, fluorescein diacetate staining and scanning electron microscopy indicated that the outer scale desiccates from the inside out. This striking observation suggests that cell death in the outer scales, during skin formation, is an internal and organized process that does not derive only from air desiccation. DNA fragmentation, a known hallmark of programmed cell death (PCD), was revealed in the outer scales and gradually decreased toward the inner scales of the bulb. Transmission electron microscopy further revealed PCD-related structural alterations in the outer scales which were absent from the inner scales. De novo transcriptome assembly for three different scales: 1st (outer), 5th (intermediate) and 8th (inner) fleshy scales identified 2,542 differentially expressed genes among them. GO enrichment for cluster analysis revealed increasing metabolic processes in the outer senescent scale related to defense response, PCD processes, carbohydrate metabolism and flavonoid biosynthesis, whereas increased metabolism and developmental growth processes were identified in the inner scales. High expression levels of PCD-related genes were identified in the outer scale compared to the inner ones, highlighting the involvement of PCD in outer-skin development. These findings suggest that a program to form the dry protective skin exists and functions only in the outer scales of onion. © 2017 Galsurker, Doron-Faigenboim, Teper-Bamnolker, Daus, Fridman, Lers and Eshel.
Scientific Publication
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