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פותח על ידי קלירמאש פתרונות בע"מ -
The fruit fly PUB: A phagostimulation unit bioassay system to quantitatively measure ingestion of baits by individual flies
Year:
2004
Source of publication :
Journal of Applied Entomology
Authors :
זילברג, ל'
;
.
נמני-לביא, אסתר
;
.
נסטל, דוד
;
.
Volume :
128
Co-Authors:
Nestel, D., Department of Entomology, Institute of Plant Protection, Volcani Center, Beit-Dagan, Israel, Institute of Plant Protection, Agricultural Research Organization, Volcani Center, P.O. Box 6, 50250 Beit Dagan, Israel
Nemny-Lavy, E., Department of Entomology, Institute of Plant Protection, Volcani Center, Beit-Dagan, Israel
Zilberg, L., Department of Entomology, Institute of Plant Protection, Volcani Center, Beit-Dagan, Israel
Weiss, M., Plant Protect. and Inspection Serv., Ministry of Agriculture, Beit-Dagan, Israel
Akiva, R., Institute of Biological Control, Citrus Marketing Board of Israel, Beit-Dagan, Israel
Gazit, Y., Institute of Biological Control, Citrus Marketing Board of Israel, Beit-Dagan, Israel
Facilitators :
From page:
576
To page:
582
(
Total pages:
7
)
Abstract:
A bioassay to investigate quantitative phagostimulation and ingestion physiology of baits on individual fruit flies is presented. The study was undertaken using two fruit fly species: the Mediterranean fruit fly (Ceratitis capitata), a cosmopolitan insect pest, and the Ethiopian fruit fly (Dacus ciliatus), a quarantine insect in Israel. Our model bait suspension included spinosad as the toxic agent, and 1% yeast hydrolysate with 10% sucrose as phagostimulant. A preliminary toxicology study showed that the two fruit flies are highly sensitive to low concentrations of spinosad baited with this phagostimulant. The maximum concentration needed to kill 90% of the female flies was 4.2 and 8.5 p.p.m. for C. capitata and D. ciliatus, respectively. The bioassay was able to detect the ingestion of low volumes (e.g. 1 μl) of tested solutions. The bioassay was also able to detect differences in intake of different concentrations of spinosad solutions and relate ingestion to fruit fly mortality. Additionally, the bioassay was sensitive enough to highlight differences in intake related to the physiological status of the fruit fly and fly species. The bioassay can also be used to follow ingestion kinetics of baits. We expect that this bioassay will contribute in the exploration of more efficient bait systems for fruit flies.
Note:
Related Files :
Bioassay
Capitata
Ceratitis capitata
chemical control
Dacus ciliatus
food intake
Insecta
pest species
עוד תגיות
תוכן קשור
More details
DOI :
10.1111/j.1439-0418.2004.00895
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
26277
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:21
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The fruit fly PUB: A phagostimulation unit bioassay system to quantitatively measure ingestion of baits by individual flies
128
Nestel, D., Department of Entomology, Institute of Plant Protection, Volcani Center, Beit-Dagan, Israel, Institute of Plant Protection, Agricultural Research Organization, Volcani Center, P.O. Box 6, 50250 Beit Dagan, Israel
Nemny-Lavy, E., Department of Entomology, Institute of Plant Protection, Volcani Center, Beit-Dagan, Israel
Zilberg, L., Department of Entomology, Institute of Plant Protection, Volcani Center, Beit-Dagan, Israel
Weiss, M., Plant Protect. and Inspection Serv., Ministry of Agriculture, Beit-Dagan, Israel
Akiva, R., Institute of Biological Control, Citrus Marketing Board of Israel, Beit-Dagan, Israel
Gazit, Y., Institute of Biological Control, Citrus Marketing Board of Israel, Beit-Dagan, Israel
The fruit fly PUB: A phagostimulation unit bioassay system to quantitatively measure ingestion of baits by individual flies
A bioassay to investigate quantitative phagostimulation and ingestion physiology of baits on individual fruit flies is presented. The study was undertaken using two fruit fly species: the Mediterranean fruit fly (Ceratitis capitata), a cosmopolitan insect pest, and the Ethiopian fruit fly (Dacus ciliatus), a quarantine insect in Israel. Our model bait suspension included spinosad as the toxic agent, and 1% yeast hydrolysate with 10% sucrose as phagostimulant. A preliminary toxicology study showed that the two fruit flies are highly sensitive to low concentrations of spinosad baited with this phagostimulant. The maximum concentration needed to kill 90% of the female flies was 4.2 and 8.5 p.p.m. for C. capitata and D. ciliatus, respectively. The bioassay was able to detect the ingestion of low volumes (e.g. 1 μl) of tested solutions. The bioassay was also able to detect differences in intake of different concentrations of spinosad solutions and relate ingestion to fruit fly mortality. Additionally, the bioassay was sensitive enough to highlight differences in intake related to the physiological status of the fruit fly and fly species. The bioassay can also be used to follow ingestion kinetics of baits. We expect that this bioassay will contribute in the exploration of more efficient bait systems for fruit flies.
Scientific Publication
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