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אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Specific combinations of human serum albumin introns direct high level expression of albumin in transfected COS cells and in the milk of transgenic mice
Year:
1994
Source of publication :
Transgenic Research
Authors :
ברש, איתמר
;
.
שני, משה
;
.
Volume :
3
Co-Authors:
Hurwitz, D.R., Rhône-Poulenc Rorer Central Research, 500 Arcola Road, Collegeville, 19426, PA, United States
Nathan, M., Rhône-Poulenc Rorer Central Research, 500 Arcola Road, Collegeville, 19426, PA, United States
Barash, I., Institute of Animal Science, ARO, The Volcani Center, Bet Dagan, 50250, Israel
Ilan, N., Institute of Animal Science, ARO, The Volcani Center, Bet Dagan, 50250, Israel
Shani, M., Institute of Animal Science, ARO, The Volcani Center, Bet Dagan, 50250, Israel
Facilitators :
From page:
365
To page:
375
(
Total pages:
11
)
Abstract:
A new series of expression vectors, each comprised of the β-lactoglobulin (BLG) promoter driving one of a variety of human serum albumin (HSA) minigenes or the entire gene, were evaluated for their ability to direct expression of HSA in vitro in COS tissue culture cells and into the milk of transgenic mice. Vectors directed a hierarchy of expression levels in vitro, dependent upon the specific complement of HSA introns included. HSA introns acted in a synergistic manner. In addition, minigenes comprised of specific subsets of introns were more efficacious than the entire HSA gene with all of its introns. Transgenic mice expressed as much as 10 mg ml-1 of HSA in their milk. Vectors comprised of specific intron subsets directed levels at 1 mg ml-1 or greater in the milk of 20% of generated transgenics. A statistical correlation between the expression level trend in vitro with the trend of expression in vivo (% which express) at detectable levels (p=0.0015) and at the level of greater than 0.1 mg ml-1 (p=0.0156) was demonstrated. A weak correlation existed (p=0.0526) at in vivo levels of 1 mg ml-1 or greater. These new vectors are expected to direct the production of high levels of HSA in the milk of a large percentage of generated transgenic dairy animals. © 1994 Chapman & Hall.
Note:
Related Files :
Animal
biosynthesis
chemistry
Female
Genetics
lactation
mice
milk
Serum Albumin
עוד תגיות
תוכן קשור
More details
DOI :
10.1007/BF01976768
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
26289
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:21
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Scientific Publication
Specific combinations of human serum albumin introns direct high level expression of albumin in transfected COS cells and in the milk of transgenic mice
3
Hurwitz, D.R., Rhône-Poulenc Rorer Central Research, 500 Arcola Road, Collegeville, 19426, PA, United States
Nathan, M., Rhône-Poulenc Rorer Central Research, 500 Arcola Road, Collegeville, 19426, PA, United States
Barash, I., Institute of Animal Science, ARO, The Volcani Center, Bet Dagan, 50250, Israel
Ilan, N., Institute of Animal Science, ARO, The Volcani Center, Bet Dagan, 50250, Israel
Shani, M., Institute of Animal Science, ARO, The Volcani Center, Bet Dagan, 50250, Israel
Specific combinations of human serum albumin introns direct high level expression of albumin in transfected COS cells and in the milk of transgenic mice
A new series of expression vectors, each comprised of the β-lactoglobulin (BLG) promoter driving one of a variety of human serum albumin (HSA) minigenes or the entire gene, were evaluated for their ability to direct expression of HSA in vitro in COS tissue culture cells and into the milk of transgenic mice. Vectors directed a hierarchy of expression levels in vitro, dependent upon the specific complement of HSA introns included. HSA introns acted in a synergistic manner. In addition, minigenes comprised of specific subsets of introns were more efficacious than the entire HSA gene with all of its introns. Transgenic mice expressed as much as 10 mg ml-1 of HSA in their milk. Vectors comprised of specific intron subsets directed levels at 1 mg ml-1 or greater in the milk of 20% of generated transgenics. A statistical correlation between the expression level trend in vitro with the trend of expression in vivo (% which express) at detectable levels (p=0.0015) and at the level of greater than 0.1 mg ml-1 (p=0.0156) was demonstrated. A weak correlation existed (p=0.0526) at in vivo levels of 1 mg ml-1 or greater. These new vectors are expected to direct the production of high levels of HSA in the milk of a large percentage of generated transgenic dairy animals. © 1994 Chapman & Hall.
Scientific Publication
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