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פותח על ידי קלירמאש פתרונות בע"מ -
Sucrose modulation of soybean Vsp gene expression is inhibited by auxin
Year:
1994
Authors :
צדקה, אבי
;
.
Volume :
104
Co-Authors:
Dewald, D.B., Dept. of Biochemistry and Biophysics, Texas A and M University, College Station, TX 77843, United States, Div. of Cell. and Molecular Medicine, Howard Hughes Medical Institute, University of California, San Diego, San Diego, CA 92093-0668, United States
Sadka, A., Dept. of Biochemistry and Biophysics, Texas A and M University, College Station, TX 77843, United States
Mullet, J.E., Dept. of Biochemistry and Biophysics, Texas A and M University, College Station, TX 77843, United States
Facilitators :
From page:
439
To page:
444
(
Total pages:
6
)
Abstract:
We have shown that auxin represses soybean (Glycine max L.) vegetative storage protein gene (Vsp) expression in suspension-cultured cells and in leaves and petioles of excised trifoliates. The auxin analog naphthyleneacetic acid (NAA) at 10 μM strongly inhibited methyl jasmonate-induced Vsp expression in soybean suspension-cultured cells. Both indole-3-acetic acid and NAA inhibited methyl jasmonate- and wound-induced expression of the Vsp and LoxA in excised soybean trifoliate leaves and petioles. The less active auxin analog phenylacetic acid had less effect on methyl jasmonate- and wound-induced expression of these genes. Addition of cytokinin to alter the auxin:cytokinin ratio did not reverse auxin inhibition of Vsp expression. Transcription of β-glucuronidase (Gus) modulated by a methyl jasmonate-responsive domain derived from the VspB promoter was minimally influenced by auxin. In contrast, sucrose-induced expression of Gus mediated by a sucrose-responsive domain of the VspB promoter was strongly inhibited by NAA. We conclude that auxin inhibits Vsp mRNA accumulation, in part, by repressing sugar-mediated activation of Vsp expression.
Note:
Related Files :
Glycine max
Loxa
עוד תגיות
תוכן קשור
More details
DOI :
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
26348
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:22
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Scientific Publication
Sucrose modulation of soybean Vsp gene expression is inhibited by auxin
104
Dewald, D.B., Dept. of Biochemistry and Biophysics, Texas A and M University, College Station, TX 77843, United States, Div. of Cell. and Molecular Medicine, Howard Hughes Medical Institute, University of California, San Diego, San Diego, CA 92093-0668, United States
Sadka, A., Dept. of Biochemistry and Biophysics, Texas A and M University, College Station, TX 77843, United States
Mullet, J.E., Dept. of Biochemistry and Biophysics, Texas A and M University, College Station, TX 77843, United States
Sucrose modulation of soybean Vsp gene expression is inhibited by auxin
We have shown that auxin represses soybean (Glycine max L.) vegetative storage protein gene (Vsp) expression in suspension-cultured cells and in leaves and petioles of excised trifoliates. The auxin analog naphthyleneacetic acid (NAA) at 10 μM strongly inhibited methyl jasmonate-induced Vsp expression in soybean suspension-cultured cells. Both indole-3-acetic acid and NAA inhibited methyl jasmonate- and wound-induced expression of the Vsp and LoxA in excised soybean trifoliate leaves and petioles. The less active auxin analog phenylacetic acid had less effect on methyl jasmonate- and wound-induced expression of these genes. Addition of cytokinin to alter the auxin:cytokinin ratio did not reverse auxin inhibition of Vsp expression. Transcription of β-glucuronidase (Gus) modulated by a methyl jasmonate-responsive domain derived from the VspB promoter was minimally influenced by auxin. In contrast, sucrose-induced expression of Gus mediated by a sucrose-responsive domain of the VspB promoter was strongly inhibited by NAA. We conclude that auxin inhibits Vsp mRNA accumulation, in part, by repressing sugar-mediated activation of Vsp expression.
Scientific Publication
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