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פותח על ידי קלירמאש פתרונות בע"מ -
Lanosterol synthase-like is involved with differential accumulation of steroidal glycoalkaloids in potato
Year:
2017
Source of publication :
Planta
Authors :
גינזברג, עידית
;
.
ויסברג, מירה
;
.
פוגלמן, עדנה
;
.
תנעמי, זכריה
;
.
Volume :
246
Co-Authors:
Kumar, A., Institute of Plant Sciences, ARO, The Volcani Center, P. O. Box 15159, Rishon LeZiyyon, Israel
Fogelman, E., Institute of Plant Sciences, ARO, The Volcani Center, P. O. Box 15159, Rishon LeZiyyon, Israel
Weissberg, M., Institute of Plant Sciences, ARO, The Volcani Center, P. O. Box 15159, Rishon LeZiyyon, Israel
Tanami, Z., Institute of Plant Sciences, ARO, The Volcani Center, P. O. Box 15159, Rishon LeZiyyon, Israel
Veilleux, R.E., Department of Horticulture, Virginia Tech, Blacksburg, VA, United States
Ginzberg, I., Institute of Plant Sciences, ARO, The Volcani Center, P. O. Box 15159, Rishon LeZiyyon, Israel
Facilitators :
From page:
1189
To page:
1202
(
Total pages:
14
)
Abstract:
Main conclusion: Phytosterol homeostasis may be maintained in leaves through diversion of intermediates into glycoalkaloid biosynthesis, whereas in tuber flesh, excess intermediates are catalyzed by tuber-specificStLAS-like, resulting in low tuber glycoalkaloids. Lanosterol synthase (LAS) and cycloartenol synthase (CAS) are phylogenetically related enzymes. Cycloartenol is the accepted precursor leading to cholesterol and phytosterols, and in potato, to steroidal glycoalkaloid (SGA) biosynthesis. LAS was also shown to synthesize some plant sterols, albeit at trace amounts, questioning its role in sterol homeostasis. Presently, a potato LAS-related gene (StLAS-like) was identified and its activity verified in a yeast complementation assay. A transgenic approach with targeted gene expression and metabolic profiling of sterols and SGAs was used. Analyses of StLAS-like transcript levels and StLAS-like-promoter::GUS reporter assays indicated specific expression in tuber flesh tissue. Overexpression of Arabidopsis AtLAS in leaves where the endogenic StLAS-like is not expressed, resulted with increased SGA level and reduced phytosterol level, while in the tuber flesh SGA level was reduced. StLAS-like expression only in tuber flesh may explain the differential accumulation of SGAs in commercial cultivars—low in tubers, high in leaves. In leaves, to maintain phytosterol homeostasis, an excess of intermediates may be diverted into SGA biosynthesis, whereas in tuber flesh these intermediates are catalyzed by tuber-specific StLAS-like instead, resulting in low levels of SGA. © 2017 Springer-Verlag GmbH Germany
Note:
Related Files :
Chaconine
Phytosterols
solanine
Solanum tuberosum
עוד תגיות
תוכן קשור
More details
DOI :
10.1007/s00425-017-2763-z
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
26378
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:22
Scientific Publication
Lanosterol synthase-like is involved with differential accumulation of steroidal glycoalkaloids in potato
246
Kumar, A., Institute of Plant Sciences, ARO, The Volcani Center, P. O. Box 15159, Rishon LeZiyyon, Israel
Fogelman, E., Institute of Plant Sciences, ARO, The Volcani Center, P. O. Box 15159, Rishon LeZiyyon, Israel
Weissberg, M., Institute of Plant Sciences, ARO, The Volcani Center, P. O. Box 15159, Rishon LeZiyyon, Israel
Tanami, Z., Institute of Plant Sciences, ARO, The Volcani Center, P. O. Box 15159, Rishon LeZiyyon, Israel
Veilleux, R.E., Department of Horticulture, Virginia Tech, Blacksburg, VA, United States
Ginzberg, I., Institute of Plant Sciences, ARO, The Volcani Center, P. O. Box 15159, Rishon LeZiyyon, Israel
Lanosterol synthase-like is involved with differential accumulation of steroidal glycoalkaloids in potato
Main conclusion: Phytosterol homeostasis may be maintained in leaves through diversion of intermediates into glycoalkaloid biosynthesis, whereas in tuber flesh, excess intermediates are catalyzed by tuber-specificStLAS-like, resulting in low tuber glycoalkaloids. Lanosterol synthase (LAS) and cycloartenol synthase (CAS) are phylogenetically related enzymes. Cycloartenol is the accepted precursor leading to cholesterol and phytosterols, and in potato, to steroidal glycoalkaloid (SGA) biosynthesis. LAS was also shown to synthesize some plant sterols, albeit at trace amounts, questioning its role in sterol homeostasis. Presently, a potato LAS-related gene (StLAS-like) was identified and its activity verified in a yeast complementation assay. A transgenic approach with targeted gene expression and metabolic profiling of sterols and SGAs was used. Analyses of StLAS-like transcript levels and StLAS-like-promoter::GUS reporter assays indicated specific expression in tuber flesh tissue. Overexpression of Arabidopsis AtLAS in leaves where the endogenic StLAS-like is not expressed, resulted with increased SGA level and reduced phytosterol level, while in the tuber flesh SGA level was reduced. StLAS-like expression only in tuber flesh may explain the differential accumulation of SGAs in commercial cultivars—low in tubers, high in leaves. In leaves, to maintain phytosterol homeostasis, an excess of intermediates may be diverted into SGA biosynthesis, whereas in tuber flesh these intermediates are catalyzed by tuber-specific StLAS-like instead, resulting in low levels of SGA. © 2017 Springer-Verlag GmbH Germany
Scientific Publication
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