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פותח על ידי קלירמאש פתרונות בע"מ -
The effects of a duplication in the ovine growth hormone (GH) gene on GH expression in the pituitaries of ram lambs from lean and fat-selected sheep lines
Year:
1997
Source of publication :
Domestic Animal Endocrinology
Authors :
גוטויין, אלישע
;
.
Volume :
14
Co-Authors:
Fleming, J.S., AgResearch, Invermay Agricultural Centre, Private Bag 50034, Mosgiel, New Zealand, Department of Physiology, Centre for Gene Research, Otago Medical School, PO Box 913, Dunedin, New Zealand
Suttie, J.M., AgResearch, Invermay Agricultural Centre, Private Bag 50034, Mosgiel, New Zealand
Montgomery, G.W., AgResearch Molecular Biology Unit, Department of Biochemistry, University of Otago, PO Box 56, Dunedin, New Zealand
Gunn, J., AgResearch, Invermay Agricultural Centre, Private Bag 50034, Mosgiel, New Zealand
Stuart, S.K., AgResearch, Invermay Agricultural Centre, Private Bag 50034, Mosgiel, New Zealand
Littlejohn, R.P., AgResearch, Invermay Agricultural Centre, Private Bag 50034, Mosgiel, New Zealand
Gootwine, E., Institute of Animal Science, A.R.O., Volcani Center, Bet Dagan 50250, Israel
Facilitators :
From page:
17
To page:
24
(
Total pages:
8
)
Abstract:
Growth hormone (GH) gene expression was investigated in pituitaries of 14- to 15 month-old ram lambs from flocks selected for high (fat) or low (lean) back fat depth, which were also homozygous for a single GH gene allele, heterozygous or homozygous for a duplication in the GH gene. The pituitaries of lean sheep of all three GH genotypes were significantly heavier than those of fat sheep, but there were no pituitary weight differences between GH genotypes. No significant lean fat selection line- or GH genotype-specific differences were measured in pituitary GH concentration. However there was a significant increase (P < 0.01) in the total pituitary content of GH in lean compared with fat animals and a significant interaction between GH genotype and lean-fat selection line (P < 0.05) was noted for GH content. No significant differences were measured in the relative concentration of GH mRNA, suggesting that the ratio of GH mRNA per mg total cellular RNA remained constant across lean-fat selection line and GH genotype. We conclude that the pituitary glands of Coopworth sheep selected for low backfat depth (lean) are bigger and have air increased GH content, but appear to contain similar concentrations of GH mRNA and immunoreactive GH as the pituitaries of fat sheep. The presence of the GH gent duplication in sheep has little measurable effect on the expression and storage of GH in the pituitary.
Note:
Related Files :
Animal
animal experiment
Animals
chemistry
Genetics
hypophysis
Male
metabolism
multigene family
obesity
sheep
עוד תגיות
תוכן קשור
More details
DOI :
10.1016/S0739-7240(96)00098-7
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
26381
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:22
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Scientific Publication
The effects of a duplication in the ovine growth hormone (GH) gene on GH expression in the pituitaries of ram lambs from lean and fat-selected sheep lines
14
Fleming, J.S., AgResearch, Invermay Agricultural Centre, Private Bag 50034, Mosgiel, New Zealand, Department of Physiology, Centre for Gene Research, Otago Medical School, PO Box 913, Dunedin, New Zealand
Suttie, J.M., AgResearch, Invermay Agricultural Centre, Private Bag 50034, Mosgiel, New Zealand
Montgomery, G.W., AgResearch Molecular Biology Unit, Department of Biochemistry, University of Otago, PO Box 56, Dunedin, New Zealand
Gunn, J., AgResearch, Invermay Agricultural Centre, Private Bag 50034, Mosgiel, New Zealand
Stuart, S.K., AgResearch, Invermay Agricultural Centre, Private Bag 50034, Mosgiel, New Zealand
Littlejohn, R.P., AgResearch, Invermay Agricultural Centre, Private Bag 50034, Mosgiel, New Zealand
Gootwine, E., Institute of Animal Science, A.R.O., Volcani Center, Bet Dagan 50250, Israel
The effects of a duplication in the ovine growth hormone (GH) gene on GH expression in the pituitaries of ram lambs from lean and fat-selected sheep lines
Growth hormone (GH) gene expression was investigated in pituitaries of 14- to 15 month-old ram lambs from flocks selected for high (fat) or low (lean) back fat depth, which were also homozygous for a single GH gene allele, heterozygous or homozygous for a duplication in the GH gene. The pituitaries of lean sheep of all three GH genotypes were significantly heavier than those of fat sheep, but there were no pituitary weight differences between GH genotypes. No significant lean fat selection line- or GH genotype-specific differences were measured in pituitary GH concentration. However there was a significant increase (P < 0.01) in the total pituitary content of GH in lean compared with fat animals and a significant interaction between GH genotype and lean-fat selection line (P < 0.05) was noted for GH content. No significant differences were measured in the relative concentration of GH mRNA, suggesting that the ratio of GH mRNA per mg total cellular RNA remained constant across lean-fat selection line and GH genotype. We conclude that the pituitary glands of Coopworth sheep selected for low backfat depth (lean) are bigger and have air increased GH content, but appear to contain similar concentrations of GH mRNA and immunoreactive GH as the pituitaries of fat sheep. The presence of the GH gent duplication in sheep has little measurable effect on the expression and storage of GH in the pituitary.
Scientific Publication
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