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פותח על ידי קלירמאש פתרונות בע"מ -
O-methyltransferases involved in the biosynthesis of volatile phenolic derivatives in rose petals
Year:
2002
Authors :
בר, עינת
;
.
לביד, נועה
;
.
לוינסון, אפרים
;
.
שליט, משה
;
.
Volume :
129
Co-Authors:

Lavid, N., Vegetable Crops, Newe Ya'ar Research Center, Agricultural Research Organization, P.O. Box 1021, Ramat, Yishay 30095, Israel
Wang, J.
Shalit, M., Vegetable Crops, Newe Ya'ar Research Center, Agricultural Research Organization, P.O. Box 1021, Ramat, Yishay 30095, Israel
Guterman, I.
Bar, E., Vegetable Crops, Newe Ya'ar Research Center, Agricultural Research Organization, P.O. Box 1021, Ramat, Yishay 30095, Israel
Beuerle, T.
Menda, N.
Shafir, S.
Zamir, D.
Adam, Z.
Vainstein, A.
Weiss, D.
Pichersky, E.
Lewinsohn, E., Vegetable Crops, Newe Ya'ar Research Center, Agricultural Research Organization, P.O. Box 1021, Ramat, Yishay 30095, Israel

Facilitators :
From page:
1899
To page:
1907
(
Total pages:
9
)
Abstract:
Rose (Rosa hybrida) flowers produce and emit a diverse array of volatiles, characteristic to their unique scent. One of the most prominent compounds in the floral volatiles of many rose varieties is the methoxylated phenolic derivative 3,5-dimethoxytoluene (orcinol dimethyl ether). Cell-free extracts derived from developing rose petals displayed O-methyltransferase (OMT) activities toward several phenolic substrates, including 3,5-dihydroxytoluene (orcinol), 3-methoxy,5-hydroxytoluene (orcinol monomethyl ether), 1-methoxy, 2-hydroxy benezene (guaiacol), and eugenol. The activity was most prominent in rose cv Golden Gate, a variety that produces relatively high levels of orcinol dimethyl ether, as compared with rose cv Fragrant Cloud, an otherwise scented variety but which emits almost no orcinol dimethyl ether. Using a functional genomics approach, we have identified and characterized two closely related cDNAs from a rose petal library that each encode a protein capable of methylating the penultimate and immediate precursors (orcinol and orcinol monomethyl ether, respectively) to give the final orcinol dimethyl ether product. The enzymes, designated orcinol OMTs (OOMT1 and OOMT2), are closely related to other plant methyltransferases whose substrates range from isoflavones to phenylpropenes. The peak in the levels of OOMT1 and OOMT2 transcripts in the flowers coincides with peak OMT activity and with the emission of orcinol dimethyl ether.
Note:
Related Files :
biosynthesis
Derivatives
DNA
Genes
Genetics
metabolism
molecular genetics
phenols
Rosa
עוד תגיות
תוכן קשור
More details
DOI :
10.1104/pp.005330
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
26425
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:22
Scientific Publication
O-methyltransferases involved in the biosynthesis of volatile phenolic derivatives in rose petals
129

Lavid, N., Vegetable Crops, Newe Ya'ar Research Center, Agricultural Research Organization, P.O. Box 1021, Ramat, Yishay 30095, Israel
Wang, J.
Shalit, M., Vegetable Crops, Newe Ya'ar Research Center, Agricultural Research Organization, P.O. Box 1021, Ramat, Yishay 30095, Israel
Guterman, I.
Bar, E., Vegetable Crops, Newe Ya'ar Research Center, Agricultural Research Organization, P.O. Box 1021, Ramat, Yishay 30095, Israel
Beuerle, T.
Menda, N.
Shafir, S.
Zamir, D.
Adam, Z.
Vainstein, A.
Weiss, D.
Pichersky, E.
Lewinsohn, E., Vegetable Crops, Newe Ya'ar Research Center, Agricultural Research Organization, P.O. Box 1021, Ramat, Yishay 30095, Israel

O-methyltransferases involved in the biosynthesis of volatile phenolic derivatives in rose petals
Rose (Rosa hybrida) flowers produce and emit a diverse array of volatiles, characteristic to their unique scent. One of the most prominent compounds in the floral volatiles of many rose varieties is the methoxylated phenolic derivative 3,5-dimethoxytoluene (orcinol dimethyl ether). Cell-free extracts derived from developing rose petals displayed O-methyltransferase (OMT) activities toward several phenolic substrates, including 3,5-dihydroxytoluene (orcinol), 3-methoxy,5-hydroxytoluene (orcinol monomethyl ether), 1-methoxy, 2-hydroxy benezene (guaiacol), and eugenol. The activity was most prominent in rose cv Golden Gate, a variety that produces relatively high levels of orcinol dimethyl ether, as compared with rose cv Fragrant Cloud, an otherwise scented variety but which emits almost no orcinol dimethyl ether. Using a functional genomics approach, we have identified and characterized two closely related cDNAs from a rose petal library that each encode a protein capable of methylating the penultimate and immediate precursors (orcinol and orcinol monomethyl ether, respectively) to give the final orcinol dimethyl ether product. The enzymes, designated orcinol OMTs (OOMT1 and OOMT2), are closely related to other plant methyltransferases whose substrates range from isoflavones to phenylpropenes. The peak in the levels of OOMT1 and OOMT2 transcripts in the flowers coincides with peak OMT activity and with the emission of orcinol dimethyl ether.
Scientific Publication
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