נגישות
menu      
חיפוש מתקדם
תחביר
חפש...
הספר "אוצר וולקני"
אודות
תנאי שימוש
ניהול
קהילה:
אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Localization of mRNAs coding for peroxisomal proteins in the yeast, Saccharomyces cerevisiae -2009
Year:
2009
Authors :
ציפור, גדי
;
.
Volume :
106
Co-Authors:
Zipor, G., Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
Haim-Vilmovsky, L., Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
Gelin-Licht, R., Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
Gadir, N., Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
Brocard, C., Max F. Perutz Laboratories, University of Vienna, A-1030 Vienna, Austria
Gerst, J.E., Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
Facilitators :
From page:
19848
To page:
19853
(
Total pages:
6
)
Abstract:
Targeted mRNA trafficking and local translation may play a significant role in controlling protein localization. Here we examined for the first time the localization of all (≈50) mRNAs encoding peroxisomal proteins (mPPs) involved in peroxisome biogenesis and function. By using the bacteriophage MS2-CP RNA-binding protein (RBP) fused to multiple copies of GFP, we demonstrated that >40 endogenously expressed mPPs tagged with the MS2 aptamer form fluorescent RNA granules in vivo. The use of different RFP-tagged organellar markers revealed 3 basic patterns of mPP granule localization: to peroxisomes, to the endoplasmic reticulum (ER), and nonperoxisomal. Twelve mPPs (i.e., PEX1, PEX5, PEX8, PEX11-15, DCI1, NPY1, PCS60, and POX1) had a high percentage (52%-80%) of mRNA colocalization with peroxisomes. Thirteen mPPs (i.e., AAT2, PEX6, MDH3, PEX28, etc.) showed a low percentage (30%-42%) of colocalization, and 1 mPP (PEX3) preferentially localized to the ER. The mPPs of the nonperoxisomal pattern (i.e., GPD1, PCD1, PEX7) showed ≪30% colocalization. mPP association with the peroxisome or ER was verified using cell fractionation and RT-PCR analysis. A model mPP, PEX14 mRNA, was found to be in close association with peroxisomes throughout the cell cycle, with its localization depending in part on the 3′-UTR, initiation of translation, and the Puf5 RBP. The different patterns of mPP localization observed suggest that multiple mechanisms involved in mRNA localization and translation may play roles in the importation of protein into peroxisomes.
Note:
Related Files :
Biogenesis
in vivo study
protein localization
Saccharomyces cerevisiae Proteins
עוד תגיות
תוכן קשור
More details
DOI :
10.1073/pnas.0910754106
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
26473
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:23
You may also be interested in
Scientific Publication
Localization of mRNAs coding for peroxisomal proteins in the yeast, Saccharomyces cerevisiae -2009
106
Zipor, G., Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
Haim-Vilmovsky, L., Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
Gelin-Licht, R., Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
Gadir, N., Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
Brocard, C., Max F. Perutz Laboratories, University of Vienna, A-1030 Vienna, Austria
Gerst, J.E., Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
Localization of mRNAs coding for peroxisomal proteins in the yeast, Saccharomyces cerevisiae
Targeted mRNA trafficking and local translation may play a significant role in controlling protein localization. Here we examined for the first time the localization of all (≈50) mRNAs encoding peroxisomal proteins (mPPs) involved in peroxisome biogenesis and function. By using the bacteriophage MS2-CP RNA-binding protein (RBP) fused to multiple copies of GFP, we demonstrated that >40 endogenously expressed mPPs tagged with the MS2 aptamer form fluorescent RNA granules in vivo. The use of different RFP-tagged organellar markers revealed 3 basic patterns of mPP granule localization: to peroxisomes, to the endoplasmic reticulum (ER), and nonperoxisomal. Twelve mPPs (i.e., PEX1, PEX5, PEX8, PEX11-15, DCI1, NPY1, PCS60, and POX1) had a high percentage (52%-80%) of mRNA colocalization with peroxisomes. Thirteen mPPs (i.e., AAT2, PEX6, MDH3, PEX28, etc.) showed a low percentage (30%-42%) of colocalization, and 1 mPP (PEX3) preferentially localized to the ER. The mPPs of the nonperoxisomal pattern (i.e., GPD1, PCD1, PEX7) showed ≪30% colocalization. mPP association with the peroxisome or ER was verified using cell fractionation and RT-PCR analysis. A model mPP, PEX14 mRNA, was found to be in close association with peroxisomes throughout the cell cycle, with its localization depending in part on the 3′-UTR, initiation of translation, and the Puf5 RBP. The different patterns of mPP localization observed suggest that multiple mechanisms involved in mRNA localization and translation may play roles in the importation of protein into peroxisomes.
Scientific Publication
You may also be interested in