Promoters of Mycoplasma capricolum ribosomal RNA operons: Identical activities but different regulation in homologous and heterologous cells

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Promoters of Mycoplasma capricolum ribosomal RNA operons: Identical activities but different regulation in homologous and heterologous cells

Year: 

1988

Source of publication :

Nucleic Acids Research

Authors :

גפני, רון

.

Volume :

Co-Authors: 

Gafny, R., Department of Cellular Biochemistry, Hebrew University-Hadassah Medical School, Jerusalem 91010, Israel
Hyman, H.C., Department of Membrane and Ultrastructure Research, Hebrew University-Hadassah Medical School, Jerusalem 91010, Israel
Razin, S., Department of Membrane and Ultrastructure Research, Hebrew University-Hadassah Medical School, Jerusalem 91010, Israel
Glaser, G., Department of Cellular Biochemistry, Hebrew University-Hadassah Medical School, Jerusalem 91010, Israel

From page: 

61

To page: 

76

(

Total pages: 

16

)

Link :

Promoters of Mycoplasma capricolum ribosomal RNA operons: Identical activities but different regulation in homologous and heterologous cells

Abstract: 

The 5' region of the rRNA operon, rrnA, of M. capricolum was cloned. Sequence analysis revealed two tRNA genes, tRNAleu and tRNAlys, upstream to the promoter of the rRNA operon. The in vivo transcription start sites of the rRNA operon and of the tRNA genes were mapped. The same promoters used by M. capricolum RNA polymerase are also recognized by E. coli RNA polymerase both in vivo and in vitro. We find that high levels of ppGpp in E. coll, resulting from amino acid starvation or from spoT mutation, activate rather than repress the transcription of the mycoplasma rrnA operon. © 1988 IRL Press Limited, Oxford, England.